The whole transcriptional profiling of cellular metabolism during adipogenesis from hMSCs

Abstract

Metabolism homeostasis plays an important role in progenitor-cell differentiation to adipocytes, but less is known about the whole transcriptional profiling of cellular metabolism during adipogenesis. We got the first insight into the whole transcriptional profiling of cellular metabolism during adipogenesis from human mesenchymal stem cells (hMSCs) by the RNA-Seq technique. There were 1,998, 2,629, 3,112, and 3,054 differentially expressed genes (DEGs) at Days 7, 14, 21, and 28, respectively, during adipogenesis. The most enriched phosphatidylinositol 3′ kinase-serine/threonine kinase (PI3K-Akt) signaling pathway stimulated and directly regulated cellular metabolism by priming glucose aerobic glycolysis, arginine and proline metabolism, glutathione metabolism, and arachidonic acid metabolism during adipogenesis, targeting the potential key genes, such as fatty acid synthase (FABP4), phosphoenolpyruvate carboxykinase 1 (PKC1), stearoyl-CoA desaturase (SCD), and solute carrier family 2 member 1 of Gluts (SLC2A1). And it confirmed PCK1 as the key player for cellular metabolism by small interfering RNA. A comprehensive understanding of cellular metabolism and its regulatory axis of the signaling pathway during adipogenesis would reveal new study and therapy targets for fat metabolism disorders. K E Y W O R D S adipogenesis, amino acid metabolism, fatty acid metabolism, glucose metabolism, lipid droplets, PI3K-Akt signaling pathway SUPPORTING INFORMATION Additional supporting information may be found online in the Supporting Information section.How to cite this article: Yi X, Liu J, Wu P, Gong Y, Xu X, Li W.The whole transcriptional profiling of cellular metabolism during adipogenesis from hMSCs.

Adipogenesis, Osteogenesis, and Chondrogenesis of Human Mesenchymal Stem/Stromal Cells: A Comparative Transcriptome Approach

“…Whole transcriptional profiling of cellular metabolism during adipogenesis from MSC explored signaling pathways and metabolism of glucose, amino acid and fatty acid. It was shown that metabolism related pathways and the PI3K−Akt signaling pathway were the most enriched pathways using GO analysis ( Yi et al, 2020b ). The PI3K−Akt signaling pathway stimulated and directly regulated cellular metabolism by targeting the potential key genes, such as FASN, PCK1, SCD, and SLC2A1 and priming glucose aerobic glycolysis, arginine and proline metabolism, glutathione metabolism, and arachidonic acid metabolism during adipogenesis ( Yi et al, 2020b ).…”

“…It was shown that metabolism related pathways and the PI3K−Akt signaling pathway were the most enriched pathways using GO analysis ( Yi et al, 2020b ). The PI3K−Akt signaling pathway stimulated and directly regulated cellular metabolism by targeting the potential key genes, such as FASN, PCK1, SCD, and SLC2A1 and priming glucose aerobic glycolysis, arginine and proline metabolism, glutathione metabolism, and arachidonic acid metabolism during adipogenesis ( Yi et al, 2020b ). Also, analyzing polysomal RNA obtained after 3 days of adipogenic induction, it was indicated a change in the energetic profile in induced compared to non-induced cells ( Drehmer et al, 2016 ).…”

Adipose‐derived stem cells regulate metabolic homeostasis and delay aging by promoting mitophagy

“…Aging is characterized by the gradual loss of physiological integrity in tissues that results in an increased risk of functional impairment and death. This degenerative process is a major risk factor for human diseases such as cancer, diabetes, and cardiovascular and neurodegenerative diseases 1,2 . Around 150 000 people die globally every day, of which about two‐thirds die from age‐related causes 3 .…”

Functional Consequences of Low Activity of Transport System A for Neutral Amino Acids in Human Bone Marrow Mesenchymal Stem Cells

“…Proline (Pro) is a non-essential amino acid of particular relevance in mesenchymal cells, since it is needed for collagen synthesis and may play regulatory functions [36][37][38][39]. Due to its structural features, proline is a good substrate of SNAT transporters, which tolerate N-alkyl substitutions, but not of other sodium-dependent transporters, with the remarkable exception of the sodium-and chloride-dependent "IMINO" transporter SIT1 (SLC6A20, [24]) that, however, has a more restricted tissue-specific and development-dependent expression than SNAT transporters [40].…”