The Whole-Genome Sequence of Plasmid-Bearing Staphylococcus argenteus Strain B3-25B from Retail Beef Liver Encodes the Type VII Secretion System and Several Virulence Factors

Neyaz, Leena; Karki, Anand B.; Fakhr, Mohamed K.

doi:10.1128/mra.00962-19

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…Recent studies have displayed the increasing emergence of S. argenteus from many countries, which has been isolated from both humans and animals [ 33 , 34 , 35 ]. Isolation of S. argenteus emphasizes its clinical significance and the importance of correct identification in clinical laboratories [ 8 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity of Molecular Characteristics among Staphylococcus argenteus Clinical Isolates (ST2250, ST2793, ST1223, and ST2198) in Northern Taiwan

et al. 2020

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Staphylococcus argenteus is an emerging pathogen that is recognized as non-pigmented Staphylococcus aureus. However, the molecular characteristics of S. argenteus and its virulence factors have not been well studied. The present study analyzed 96 isolates of S. argenteus recovered from blood. Identification of S. argenteus was based on results of MALDI-TOF MS and lacking crtM gene. All 96 isolates were methicillin-susceptible. Multilocus sequence typing (MLST) revealed four sequence types: ST2250 (n = 72), ST2793 (n = 12), ST1223 (n = 10), and ST2198 (n = 2). All 72 ST2250 isolates harbored CRISPR loci with polymorphism of direct repeats and spacers, but no other STs carried CRISPR loci. To date, ST2793 isolates have rarely been reported in other countries. Collagen-binding adhesin gene (cna) and staphylococcal enterotoxin type C (sec) were detected in 12 (100%) and 8 (67%) ST2793 isolates, respectively. ST1223 has been reported as food poisoning pathogens, and enterotoxin gene clusters (egc) were detected in all 10 isolates, while seb gene was detected in three isolates. Two ST2198 isolates carried bone sialoprotein-binding protein gene (bbp), belonging to agr type IV. Our focus on the heterogeneity of molecular characterization in four ST types of S. argenteus revealed that S. argenteus had been isolated as early as 2000. Each ST type of S. argenteus harbors particular genetic markers that may contribute to their virulence.

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Section: Discussionmentioning

confidence: 99%

Heterogeneity of Molecular Characteristics among Staphylococcus argenteus Clinical Isolates (ST2250, ST2793, ST1223, and ST2198) in Northern Taiwan

et al. 2020

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“…The 10 S. aureus strains used in this study were previously isolated from retail beef (n = 3), chicken (n = 4), turkey (n = 2), and pork (n = 1); all strains contained plasmid DNA based on alkaline lysis and PFGE (Table 1; Abdalrahman and Fakhr, 2015;Abdalrahman et al, 2015a,b). S. aureus strains were selected to represent various retail meat sources (beef, beef liver, chicken, chicken liver, chicken gizzard, pork, and turkey), different plasmid sizes previously determined by PFGE, and various plasmid rep types (Neyaz, 2019;Neyaz et al, 2020). DNA isolation and sequencing were carried out as described previously with minor modifications Fakhr, 2016a,b,c, 2017a,b;Neyaz et al, 2019).…”

Section: Bacterial Cultures and Dna Isolationmentioning

confidence: 99%

“…S. aureus strains were selected to represent various retail meat sources (beef, beef liver, chicken, chicken liver, chicken gizzard, pork, and turkey), different plasmid sizes previously determined by PFGE, and various plasmid rep types (Neyaz, 2019;Neyaz et al, 2020). DNA isolation and sequencing were carried out as described previously with minor modifications Fakhr, 2016a,b,c, 2017a,b;Neyaz et al, 2019). Briefly, S. aureus cells were grown in tryptic soy agar (TSA) at 37 • C for 16-24 h and then used for genomic and plasmid DNA isolation.…”

Section: Bacterial Cultures and Dna Isolationmentioning

confidence: 99%

Comparative Genomics of Plasmid-Bearing Staphylococcus aureus Strains Isolated From Various Retail Meats

2020

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Food poisoning due to the consumption of Staphylococcus aureus contaminated food is a major health problem worldwide. In this study, we sequenced the genomes of ten plasmid-bearing S. aureus strains isolated from retail beef, chicken, turkey, and pork. The chromosomes of the strains varied in size from 2,654,842 to 2,807,514 bp, and a total of 25 plasmids were identified ranging from 1.4 to 118 kb. Comparative genomic analysis revealed similarities between strains isolated from the same retail meat source, indicating an origin-specific genomic composition. Genes known to modulate attachment, invasion, and toxin production were identified in the 10 genomes. Strains from retail chicken resembled human clinical isolates with respect to virulence factors and genomic islands, and retail turkey and pork isolates shared similarity with S. aureus from livestock. Most chromosomes contained antimicrobial resistance, heavy metal resistance, and stress response genes, and several plasmids contained genes involved in antimicrobial resistance and virulence. In conclusion, the genomes of S. aureus strains isolated from retail meats showed an origin-specific composition and contained virulence and antimicrobial resistance genes similar to those present in human clinical isolates.

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Mining of novel target genes through Pan-genome analysis for Conventional PCR and Real-Time PCR detection of Staphylococcus argenteus in food

Huang,

Dai,

Liu

et al. 2024

Food Control

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The Whole-Genome Sequence of Plasmid-Bearing Staphylococcus argenteus Strain B3-25B from Retail Beef Liver Encodes the Type VII Secretion System and Several Virulence Factors

Abstract: The whole-genome sequence of Staphylococcus argenteus strain B3-25B, isolated from retail beef liver, comprises a circular chromosome (2,676,222 bp) and a single plasmid (21,570 bp). The chromosome harbors genes encoding the type VII secretion system and several virulence factors.

Cited by 3 publications

References 16 publications

Heterogeneity of Molecular Characteristics among Staphylococcus argenteus Clinical Isolates (ST2250, ST2793, ST1223, and ST2198) in Northern Taiwan

Heterogeneity of Molecular Characteristics among Staphylococcus argenteus Clinical Isolates (ST2250, ST2793, ST1223, and ST2198) in Northern Taiwan

Comparative Genomics of Plasmid-Bearing Staphylococcus aureus Strains Isolated From Various Retail Meats

Mining of novel target genes through Pan-genome analysis for Conventional PCR and Real-Time PCR detection of Staphylococcus argenteus in food

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