The white collar complex is essential for sexual reproduction but dispensable for conidiation and invasive growth inFusarium verticillioides

Abstract

Fvwc1 and Fvwc2, orthologues of the wc-1 and wc-2 genes encoding for proteins of the white collar complex (WCC) in Neurospora crassa were cloned from Fusarium verticillioides and lack-of-function wc mutants were obtained by targeted gene disruption. Photo-conidiation was found to be absent in F. verticillioides, on the contrary, the wild type strain produced less conidia under continuous illumination than in the dark. Inactivation of any of the wc genes led to total female sterility, without affecting male fertility or asexual conidiation. no loss in colonization capability/invasive growth of the wc mutants was observed, when assessed on tomato fruits. Both Fvwc1 and Fvwc2 showed constitutive expression in the wild type cultures incubated in the dark and exposure to light caused only negligible increases in their transcription. Both Fvwc1 and Fvwc2 were down-regulated in a ∆Fvmat1-2-1 gene disruption mutant, lacking a functional mating type (mat1-2-1) gene, suggesting that the MAt1-2-1 product has a positive regulatory effect on the white collar genes.

Extraction and characterisation of proteins from a Malaysian Isolate of Magnaporthe grisea

“…Aconitase has been reported in various pathogens where it is involved in the production of oxalates for the Krebs cycle (Rio et al, 2008). The white-collar protein 2 (WCC) is an important component in producing light inputs for various types of blue light, including light chronobiology in the circadian rhythm, adaptation and survival of fungi in the environment, oxidative stress control, hypha development, and secondary metabolite formation (Rodriguez-Romero et al, 2010;Ruger-Herreros et al, 2011;Bodor et al, 2013). Finally, we have Succinyl CoA that participates in the site of the itaconate degradation pathway, which is important for the pathogenicity, and survival of pathogens in the host (Sasikaran et al, 2014).…”