The wheat transcription factor, TabHLH39, improves tolerance to multiple abiotic stressors in transgenic plants

Zhai, Yiqian; Zhang, Lichao; Xia, Chuan; Fu, Silu; Zhao, Guangyao; Jia, Jizeng; Kong, Xiuying

doi:10.1016/j.bbrc.2016.04.071

Cited by 66 publications

(35 citation statements)

References 30 publications

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“…Many plant genes are induced by biological and abiotic stresses, such as insects, fungi, drought, and high-salinity. Not only may these genes function in stress responses, but in stress tolerance as well [ 43 , 44 ]. Gene-expression patterns are often associated with gene function [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…qRT-PCR was performed using SYBR® Green Realtime PCR Master Mix (Toyobo, Japan) in an iQ5 (Bio-Rad) thermocycler. The amplification conditions were as follows: denaturation at 95 °C for 10 min, followed by 40 cycles of 95 °C for 15 s and 55 °C for 15 s. Relative quantitation of gene expression was performed using CsEF1α (GenBank Accession Number: XM_004138916) as control [ 43 ]. Four replicates were used for each treatment.…”

Section: Methodsmentioning

confidence: 99%

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Expression and functional analysis of the Propamocarb-related gene CsDIR16 in cucumbers

et al. 2018

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BackgroundCucumber downy mildew is among the most important diseases that can disrupt cucumber production. Propamocarb, also known as propyl-[3-(dimethylamino)propyl]carbamate (PM), is a systemic carbamate fungicide pesticide that is widely applied in agricultural production because of its high efficiency of pathogens control, especially cucumber downy mildew. However, residual PM can remain in cucumbers after the disease has been controlled. To explore the molecular mechanisms of PM retention, cucumber cultivars ‘D9320’ (with the highest residual PM content) and ‘D0351’ (lowest residual PM content) were studied. High-throughput tag-sequencing (Tag-Seq) results showed that the CsDIR16 gene was related to PM residue, which was verified using transgenic technology.ResultsWe investigated the activity of a dirigent cucumber protein encoded by the CsDIR16 in gene response to stress induced by PM treatment. Gene-expression levels of CsDIR16 were up-regulated in the fruits, leaves, and stems of ‘D0351’ plants in response to PM treatment. However, in cultivar ‘D9320’, CsDIR16 levels were down-regulated in the leaves and stems after PM treatment, with no statistically significant differences observed in the fruits. Induction by jasmonic acid, abscisic acid, polyethylene glycol 4000, NaCl, and Corynespora cassiicola Wei (Cor) resulted in CsDIR16 up-regulation in ‘D0351’ and ‘D9320’. Expression after salicylic acid treatment was up-regulated in ‘D0351’, but was down-regulated in ‘D9320’. CsDIR16 overexpression lowered PM residues, and these were more rapidly reduced in CsDIR16(+) transgenic ‘D9320’ plants than in wild-type ‘D9320’ and CsDIR16(−) transgenic plants.ConclusionsAnalyses of the CsDIR16-expression patterns in the cucumber cultivars with the highest and lowest levels of PM residue, and transgenic validation indicated that CsDIR16 plays a positive role in reducing PM residues. The findings of this study help understand the regulatory mechanisms occurring in response to PM stress in cucumbers and in establishing the genetic basis for developing low-pesticide residue cucumber cultivars.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1236-2) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Expression and functional analysis of the Propamocarb-related gene CsDIR16 in cucumbers

et al. 2018

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“…In wheat, gene expression analysis showed that TabHLH39 is induced in response to salt, polyethylene glycol, and cold, and exhibits differential expression patterns in roots, stems, and leaves. Transgenic Arabidopsis with overexpressed TabHLH39 displayed significant resistance to multiple abiotic stress conditions during the seedling stage via altered physiological indices [ 47 ]. Very recently, FtbHLH2 , cloned from Tartary buckwheat was reported to elevate cold tolerance, as observed with higher photosynthetic efficiency and lower reactive oxygen species (ROS) in transgenic plants under cold stress [ 48 ].…”

Section: Transcription Factors Involved In Abiotic Stress Responsementioning

confidence: 99%

Revisiting the Role of Plant Transcription Factors in the Battle against Abiotic Stress

Khan

Wang

et al. 2018

IJMS

193

118

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Owing to diverse abiotic stresses and global climate deterioration, the agricultural production worldwide is suffering serious losses. Breeding stress-resilient crops with higher quality and yield against multiple environmental stresses via application of transgenic technologies is currently the most promising approach. Deciphering molecular principles and mining stress-associate genes that govern plant responses against abiotic stresses is one of the prerequisites to develop stress-resistant crop varieties. As molecular switches in controlling stress-responsive genes expression, transcription factors (TFs) play crucial roles in regulating various abiotic stress responses. Hence, functional analysis of TFs and their interaction partners during abiotic stresses is crucial to perceive their role in diverse signaling cascades that many researchers have continued to undertake. Here, we review current developments in understanding TFs, with particular emphasis on their functions in orchestrating plant abiotic stress responses. Further, we discuss novel molecular mechanisms of their action under abiotic stress conditions. This will provide valuable information for understanding regulatory mechanisms to engineer stress-tolerant crops.

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“…Recently, numerous bHLH TFs in plants have been suggested to respond to diverse abiotic stresses and improve plant stress tolerance, including to cold, salt and drought (Sun et al, 2018). Under salt stress, bHLH39 increased the expression levels of stress-response genes in wheat , and thus improved the salt tolerance of bHLH39-overexpressing wheat plants (Zhai et al, 2016). A total of 18 bHLH genes in poplar have been found to respond to salt stress (Zhao et al, 2018a).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #2 of "Genome-wide analyses of the bHLH gene family reveals structural and functional characteristics in the aquatic plant Nelumbo nucifera (v0.1)"

2019

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Lotus (Nelumbo nucifera Gaertn.) is an economically important aquatic plant with multiple applications, but water salinity and cold stress seriously affect lotus yield and distribution. The basic helix-loop-helix (bHLH) transcription factors play a vital role in plant growth and development, metabolic regulation processes and responses to environmental changes. However, systematic analyses of the bHLH transcription factor family in lotus has not yet been reported. Here, we report the identification and description of bHLH genes in lotus (NnbHLHs) with a focus on functional prediction, particularly for those involved in stress resistance. In all, 115 NnbHLHs were identified in the lotus genome and classified into 19 subfamilies. The chromosomal distribution, physicochemical properties, bHLH domain, conserved motif compositions and evolution of these 115 NnbHLHs were further analyzed. To better understand the functions of the lotus bHLH family, gene ontology, cis-element, and phylogenetic analyses were conducted. NnbHLHs were predicted to be involved in plant development, metabolic regulation and responses to stress, in accordance with previous findings. Overall, 15 NnbHLHs were further investigated with functional prediction via quantitative real-time PCR (qRT-PCR) analyses. Meanwhile, expression profiles of NnbHLHs in four tissues indicated that many NnbHLHs showed tissue preference in their expression. This study is supposed to provide a good foundation for further research into the functions and evolution of NnbHLHs, and identifies candidate genes for stress resistance in lotus.

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The wheat transcription factor, TabHLH39, improves tolerance to multiple abiotic stressors in transgenic plants

Cited by 66 publications

References 30 publications

Expression and functional analysis of the Propamocarb-related gene CsDIR16 in cucumbers

Expression and functional analysis of the Propamocarb-related gene CsDIR16 in cucumbers

Revisiting the Role of Plant Transcription Factors in the Battle against Abiotic Stress

Peer Review #2 of "Genome-wide analyses of the bHLH gene family reveals structural and functional characteristics in the aquatic plant Nelumbo nucifera (v0.1)"

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