The wheat PKABA1-interacting factor TaABF1 mediates both abscisic acid-suppressed and abscisic acid-induced gene expression in bombarded aleurone cells

Johnson, Russell R.; Shin, Margaret; Shen, J

doi:10.1007/s11103-008-9354-0

Cited by 37 publications

(46 citation statements)

References 43 publications

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“…The basic information on these 35 TabZIP genes is shown in Table 1. In addition to these 35 novel TabZIP genes, Subgroup A of the wheat bZIP family included TaABF1, TaABI5, WABI5 ( WABI5-1, WABI5-2 , and WABI5-3 ), TaABL1 , and TabZIP60 ( TabZIP60-A, TabZIP60-B , and TabZIP60-D ) (Johnson et al, 2002, 2008; Kobayashi et al, 2008; Rikiishi et al, 2010; Harris et al, 2013; Xu et al, 2014; Zhang et al, 2015). Therein, TaABF1, WABI5-3 , and TabZIP60-A were identical to TabZIP23, TabZIP162 , and TabZIP51 in our nomenclature, respectively.…”

Section: Resultsmentioning

confidence: 99%

Bioinformatic Analyses of Subgroup-A Members of the Wheat bZIP Transcription Factor Family and Functional Identification of TabZIP174 Involved in Drought Stress Response

Li¹,

Feng²,

Zhang

et al. 2016

Front. Plant Sci.

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Extensive studies in Arabidopsis and rice have demonstrated that Subgroup-A members of the bZIP transcription factor family play important roles in plant responses to multiple abiotic stresses. Although common wheat (Triticum aestivum) is one of the most widely cultivated and consumed food crops in the world, there are limited investigations into Subgroup A of the bZIP family in wheat. In this study, we performed bioinformatic analyses of the 41 Subgroup-A members of the wheat bZIP family. Phylogenetic and conserved motif analyses showed that most of the Subgroup-A bZIP proteins involved in abiotic stress responses of wheat, Arabidopsis, and rice clustered in Clade A1 of the phylogenetic tree, and shared a majority of conserved motifs, suggesting the potential importance of Clade-A1 members in abiotic stress responses. Gene structure analysis showed that TabZIP genes with close phylogenetic relationships tended to possess similar exon–intron compositions, and the positions of introns in the hinge regions of the bZIP domains were highly conserved, whereas introns in the leucine zipper regions were at variable positions. Additionally, eleven groups of homologs and two groups of tandem paralogs were also identified in Subgroup A of the wheat bZIP family. Expression profiling analysis indicated that most Subgroup-A TabZIP genes were responsive to abscisic acid and various abiotic stress treatments. TabZIP27, TabZIP74, TabZIP138, and TabZIP174 proteins were localized in the nucleus of wheat protoplasts, whereas TabZIP9-GFP fusion protein was simultaneously present in the nucleus, cytoplasm, and cell membrane. Transgenic Arabidopsis overexpressing TabZIP174 displayed increased seed germination rates and primary root lengths under drought treatments. Overexpression of TabZIP174 in transgenic Arabidopsis conferred enhanced drought tolerance, and transgenic plants exhibited lower water loss rates, higher survival rates, higher proline, soluble sugar, and leaf chlorophyll contents, as well as more stable osmotic potential under drought conditions. Additionally, overexpression of TabZIP174 increased the expression of stress-responsive genes (RD29A, RD29B, RAB18, DREB2A, COR15A, and COR47). The improved drought resistance might be attributed to the increased osmotic adjustment capacity. Our results indicate that TabZIP174 may participate in regulating plant response to drought stress and holds great potential for genetic improvement of abiotic stress tolerance in crops.

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Section: Resultsmentioning

confidence: 99%

Bioinformatic Analyses of Subgroup-A Members of the Wheat bZIP Transcription Factor Family and Functional Identification of TabZIP174 Involved in Drought Stress Response

Li¹,

Feng²,

Zhang

et al. 2016

Front. Plant Sci.

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“…This direct eVect on hormone levels can make it diYcult to distinguish whether a signaling pathway inXuences only the biosynthesis of the other or whether the signaling pathway itself interacts with the signaling pathway of the other. Support that the two signaling pathways interact with each other comes from evidence that GA and ABA have antagonistic eVects on the promoters of speciWc genes (Gomez-Cadenas et al 2001;Xie et al 2006;Oh et al 2007;Zentella et al 2007;Johnson et al 2008;Sawada et al 2008). Furthermore, global transcript proWling in both Arabidopsis and cereals conWrms that many GA-regulated genes contain both GA-and ABA-responsive promoter elements (Ogawa et al 2003;Cao et al 2006;Xie et al 2006;Sreenivasulu et al 2008).…”

Section: Interaction Of Ga and Other Hormone Signaling Pathwaysmentioning

confidence: 99%

Gibberellin signaling

Hartweck¹

2008

Planta

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This review covers recent advances in gibberellin (GA) signaling. GA signaling is now understood to hinge on DELLA proteins. DELLAs negatively regulate GA response by activating the promoters of several genes including Xerico, which upregulates the abscisic acid pathway which is antagonistic to GA. DELLAs also promote transcription of the GA receptor, GIBBERELLIN INSENSITIVE DWARF 1 (GID1) and indirectly regulate GA biosynthesis genes enhancing GA responsiveness and feedback control. A structural analysis of GID1 provides a model for understanding GA signaling. GA binds within a pocket of GID1, changes GID1 conformation and increases the affinity of GID1 for DELLA proteins. GA/GID1/DELLA has increased affinity for an F-Box protein and DELLAs are subsequently degraded via the proteasome. Therefore, GA induces growth through degradation of the DELLAs. The binding of DELLA proteins to three of the PHYTOCHROME INTERACTING FACTOR (PIF) proteins integrates light and GA signaling pathways. This binding prevents PIFs 3, 4, and 5 from functioning as positive transcriptional regulators of growth in the dark. Since PIFs are degraded in light, these PIFs can only function in the combined absence of light and presence of GA. New analyses suggest that GA signaling evolved at the same time or just after the plant vascular system and before plants acquired the capacity for seed reproduction. An analysis of sequences cloned from Physcomitrella suggests that GID1 and DELLAs were the first to evolve but did not initially interact. The more recently diverging spike moss Selaginella has all the genes required for GA biosynthesis and signaling, but the role of GA response in Selaginella physiology remains a mystery.

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“…It has a predicted molecular weight of 38 kDa and is up-regulated at the transcript level by osmotic, dehydration, and cold treatments in root, shoot and scutellar tissue (Holappa and Walker-Simmons, 1995). PKABA1 appears to be involved in antagonizing gibberellic acid-induced gene expression, down-regulating, for example, α-amylase genes in the barley ( Hordeum vulgare ) aleurone layer, and in the up-regulation of genes in response to ABA (Gomez-Cadenas et al , 1999; Johnson et al , 2008). TaABF, a wheat seed AREBP, has been shown to interact with PKABA1, and PKABA1 will phosphorylate three peptides with amino acid sequences corresponding to putative phosphorylation sites in TaABF (Johnson et al , 2002).…”

Section: Introductionmentioning

confidence: 99%

Evidence that abscisic acid promotes degradation of SNF1-related protein kinase (SnRK) 1 in wheat and activation of a putative calcium-dependent SnRK2

Coello

Hirano

Hey

et al. 2011

Journal of Experimental Botany

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Sucrose nonfermenting-1 (SNF1)-related protein kinases (SnRKs) form a major family of signalling proteins in plants and have been associated with metabolic regulation and stress responses. They comprise three subfamilies: SnRK1, SnRK2, and SnRK3. SnRK1 plays a major role in the regulation of carbon metabolism and energy status, while SnRKs 2 and 3 have been implicated in stress and abscisic acid (ABA)-mediated signalling pathways. The burgeoning and divergence of this family of protein kinases in plants may have occurred to enable cross-talk between metabolic and stress signalling, and ABA-response-element-binding proteins (AREBPs), a family of transcription factors, have been shown to be substrates for members of all three subfamilies. In this study, levels of SnRK1 protein were shown to decline dramatically in wheat roots in response to ABA treatment, although the amount of phosphorylated (active) SnRK1 remained constant. Multiple SnRK2-type protein kinases were detectable in the root extracts and showed differential responses to ABA treatment. They included a 42 kDa protein that appeared to reduce in response to 3 h of ABA treatment but to recover after longer treatment. There was a clear increase in phosphorylation of this SnRK2 in response to the ABA treatment. Fractions containing this 42 kDa SnRK2 were shown to phosphorylate synthetic peptides with amino acid sequences based on those of conserved phosphorylation sites in AREBPs. The activity increased 8-fold with the addition of calcium chloride, indicating that it is calcium-dependent. The activity assigned to the 42 kDa SnRK2 also phosphorylated a heterologously expressed wheat AREBP.

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The wheat PKABA1-interacting factor TaABF1 mediates both abscisic acid-suppressed and abscisic acid-induced gene expression in bombarded aleurone cells

Cited by 37 publications

References 43 publications

Bioinformatic Analyses of Subgroup-A Members of the Wheat bZIP Transcription Factor Family and Functional Identification of TabZIP174 Involved in Drought Stress Response

Bioinformatic Analyses of Subgroup-A Members of the Wheat bZIP Transcription Factor Family and Functional Identification of TabZIP174 Involved in Drought Stress Response

Gibberellin signaling

Evidence that abscisic acid promotes degradation of SNF1-related protein kinase (SnRK) 1 in wheat and activation of a putative calcium-dependent SnRK2

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