The wheat mitochondrial gene for subunit I of the NADH dehydrogenase complex: A trans-splicing model for this gene-in-pieces

Chapdelaine, Yvan; Bonen, Linda

doi:10.1016/0092-8674(91)90464-a

Cited by 209 publications

(106 citation statements)

References 42 publications

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“…Interesting result was obtained for another polycistronic unit in wheat nad6-nad1 exon 4 (Haouazine et al, 1993). Wheat nad1 gene consists of five exons, which are scattered throughout wheat mitochondrial genome, and exons 4 and 5 are separated by trans-spliced intron (Chapdelaine and Bonen, 1991). Our result indicated that nad6 showed constant steady state transcript abundance but the level of nad1 transcript decreased during the third day.…”

Section: Studymentioning

confidence: 48%

Mitochondrial biogenesis as revealed by mitochondrial transcript profiles during germination and early seedling growth in wheat

et al. 2007

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Germination of imbibed embryos is the initial stage of plant development that is accompanied by the burst of mitochondrial respiration. To understand the process of mitochondrial biogenesis during this critical stage in wheat development, we monitored changes in mitochondrial transcript profiles during the first 3 days by adopting a newly devised macroarray system. The whole experiment was conducted in the dark to avoid influences of photosynthesis. Dry quiescent embryos started respiration rapidly after imbibition and the rate of oxygen uptake increased to peak at the first day followed by a continuous decrease until the third day under this condition. Both the cytochrome and alternative electron transport pathways appeared to contribute to this initial burst. Shoot and root growth was also remarkable during this period. Mitochondrial transcriptome was studied by macroarray analysis using 28 mitochondrial protein-coding genes, 4 nuclear encoded mitochondria-targeted genes and 2 nuclear genes as control. All transcripts were present in dry embryos at different initial levels, and a large variability was observed in their abundance among individual genes throughout the tested period. Gene expression was categorized into four clusters according to the profiles of individual transcript accumulation. A majority of the genes encoding subunits of the respiratory complexes belonged to two major clusters, the time course of transcript accumulation of one cluster agreeing with that of respiratory development and the other remaining at high constant levels. The macroarray system devised in this study should be useful in monitoring mitochondrial biogenesis under various growth conditions and at different developmental stages in cereals.

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Section: Studymentioning

confidence: 48%

Mitochondrial biogenesis as revealed by mitochondrial transcript profiles during germination and early seedling growth in wheat

et al. 2007

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“…Amino acid sequences encoded in the homologous regions of the mitochondrial nadl genes of Oenothera . wheat (Chapdelaine and Bonen, 1991). petunia (Conklin et al, 1991), Neurospora (Burger, 1985).…”

Section: Editing Results In Restoration Of Codons For Conserved Aminomentioning

confidence: 99%

Internal editing of the maize chloroplast ndhA transcript restores codons for conserved amino acids.

et al. 1992

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“…rm26 (Stern et al, 1982), rmZ8 and rrn5 (Chao et al, 1984), nadl (Chapdelaine and Bonen, 1991), atpA (Braun and Levings, 1985), atp6 (Dewey et al, 1985), atp9 (Young et al, 1986), coxI (Bonen et al, 1987), coxII (Fox and Leaver, 1981), and cob (Boer et al, 1985). To identify a species-specific RFLP in ctDNA, oligonucleotide-labeled probes were prepared from gel-purified inserts of a recombinant clone carrying a 27-kb Sal1 fragment from tomato ctDNA (Bonnema et al, 1991).…”

Section: N a Extraction And Southern Analysismentioning

confidence: 99%

Molecular and Ultrastructural Analysis of a Nonchromosomal Variegated Mutant (Tomato Mitochondrial Mutants That Cause Abnormal Leaf Development)

et al. 1995

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Mutants were recovered i n a population of cybrids formed following protoplast fusion between tomato (Lycopersicon esculentum Mill.) cv UC82 and Lycopersicon pennellii Corr. The cybrids were identified as individuals with recombinant cytoplasmic genomes but only tomato nuclear genomes. The mutants were identified based on two features, a variegated sectoring of light and dark green regions on their leaves, stems, and fruit, and reduced growth in the field. The mutants produced 50% of the shoot fresh weight and 20% of the fruit fresh weight of the parenta1 type, UC82. The variegated sectoring was maternally inherited. The chloroplast genome in the mutants was indistinguishable from the chloroplast genome in UC82, when distribution of restriction endonuclease sites was used as an assay. The mitochondrial genome i n the mutants, however, was recombinant, containing genes from UC82 and L. pennellii. Light microscopic analysis of the leaves of the mutants demonstrated an absence of the palisade layer i n the variegated sectors. Electron microscopic analysis of these same regions demonstrated an absence of normal inner membranes in the mitochondria of these cells.

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The wheat mitochondrial gene for subunit I of the NADH dehydrogenase complex: A trans-splicing model for this gene-in-pieces

Cited by 209 publications

References 42 publications

Mitochondrial biogenesis as revealed by mitochondrial transcript profiles during germination and early seedling growth in wheat

Mitochondrial biogenesis as revealed by mitochondrial transcript profiles during germination and early seedling growth in wheat

Internal editing of the maize chloroplast ndhA transcript restores codons for conserved amino acids.

Molecular and Ultrastructural Analysis of a Nonchromosomal Variegated Mutant (Tomato Mitochondrial Mutants That Cause Abnormal Leaf Development)

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