The wheat cytochrome oxidase subunit II gene has an intron insert and three radical amino acid changes relative to maize

Bonen, Linda; Boer, Poppo H.; Gray, Michael W.

doi:10.1002/j.1460-2075.1984.tb02168.x

Cited by 154 publications

(76 citation statements)

References 28 publications

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“…Clone pMNS 160 hybridized to the 3' maize probe (probe 3), but not to the wheat 3' exon, probe 4 (Table I). This is because probe 3 contains 670 bp of the 3' noncoding region of the maize COXII gene, whereas the probe 3 only extends 170 bp into the 3' noncoding region of wheat.…”

Section: Methodsmentioning

confidence: 99%

“…Clone pMNS217 hybridized to the 3' maize probe (probe 3), but did not hybridize to the 3' wheat exon, probe 4 (Table I). This is because the maize 3' end probe contains an additional 427 bp region of the maize intron which is not present on the wheat 3' exon probe, suggesting pMNS217 contains regions which were homologous to the maize intron.…”

Section: Methodsmentioning

confidence: 99%

“…That is the case in all the inserts mentioned above. Bonen et al (4) and Kao et al (9) suggest that the inserts in the wheat and rice introns may have arisen from a transpositional event. We have identified the same inverted and direct repeats by sequencing the 0.42 bp BamHI fragment of the carrot COXII gene.…”

Section: Methodsmentioning

confidence: 99%

“…This region is interrupted by a 422 bp insert (wheat intron-insert) at 1062 bp from the start codon (4). Rice contains a 1217 bp region that is 98% homologous to the wheat COXII intron and it contains a 52 bp insertion at 1318 bp from the start codon.…”

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confidence: 99%

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Cytochrome Oxidase Subunit II Gene from Carrot Contains an Intron

Turano¹,

DeBonte²,

Wilson³

et al. 1987

Plant Physiol.

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Introhs in the cytochrome oxidase subunit II (COXII) gene of plant mitochondrial DNA (mtDNA) have been observed only in monocots. The COXII genes in dicots investigated to date do not contain introns. This is the rirst report of an intron in the COXII gene of a dicot. The presence ofan intron in the carrot COXII intron was verified by restriction mapping and hybridization using specific maize and wheat COXII probes. Regions of the carrot COXII intron are homologous to the maize COXII intron and homologous to the wheat COXII intron-insert as demonstrated by hybridization. Homology of these regions was confirmed by sequencing portions of the gene. A comparison of the restriction map of the carrot COXII gene with the restriction maps of the COXII genes from pea, Oeaothera, maize, wheat, and rice revealed that the carrot map coincides with the rice restriction map.Cyt oxidase, an important part of the respiratory chain, is located in the inner mitochondrial membrane and directs the transfer of electrons from Cyt c to 02. The enzyme complex is composed of seven subunits. Three subunits (I, II, and III) are encoded by mitochondrial DNA, while the other four subunits (IV, V, VI, and VII) are encoded by nuclear DNA.The nucleotide sequence of the COXII gene from five plants, pea (12), Oenothera (8), maize (7), wheat (4), and rice (9), is highly conserved. Nucleotide homology of the COXII gene within monocots is 99%, within dicots is 89%, and between the two groups is 89%. The monocots, maize (7), wheat (4), rice (9), and rye (4), have introns in the COXII gene, while the dicots, pea (12), Oenothera (8), broad bean (4), and cucumber (4) do not contain introns. The COXII genes of all three monocots contain introns which begin at 391 bp;4 however, the introns vary in size (Fig. 1)

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Cytochrome Oxidase Subunit II Gene from Carrot Contains an Intron

Turano¹,

DeBonte²,

Wilson³

et al. 1987

Plant Physiol.

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“…This clone had already been mapped and contains, near fragment W, the second exon of the wheat mitochondrial cox11 gene [31] and the gene coding for tRNAAse (321. In a 3.4-kb PstI fragment internal to H2, a tRNAv' gene [32] had already been described and is located upstream of two exons of the spliced nad2 gene [33] (Fig.…”

Section: Localization and Sequence Of The Wheat Mitochondrial Nad9 Genementioning

confidence: 99%

Higher plant mitochondria encode an homologue of the nuclear‐encoded 30‐kDa subunit of bovine mitochondrial complex I

Lamattina

González

Gualberto

et al. 1993

European Journal of Biochemistry

144

100

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We describe the structure and expression of a wheat mitochondrial gene, which codes for a subunit of mitochondrial NADH dehydrogenase. The deduced protein sequence has 70% similarity to the 30-kDa subunit of bovine mitochondrial complex I and 65% similarity to the 31-kDa subunit of Neurospora crassa complex I, components of the iron-sulfur-protein fraction, both nuclear-encoded proteins. We named this wheat mitochondrial gene as nad9. The wheat nad9 gene is transcribed in a single mRNA of 0.9 kb that is edited (C-to-U conversions) in 14 positions. Transcript mapping revealed that the first ATG codon is just 20 nucleotides downstream of the mRNA 5' end and that the 3' end is just 23 nucleotides downstream of the nad9 stop codonThe expression of the nad9 gene in plant mitochondria was studied. Polyclonal antibodies prepared against a wheat NAD9 fusion protein specifically recognise the 30-kDa subunit of bovine mitochondrial complex I and a 27.5-kDa protein in the membrane fractions of wheat, maize and common bean mitochondria, whereas the same serum recognizes a 30-kDa protein in the mitochondria of pea, chickpea and lentil.Higher plants contain the larger mitochondrial genomic DNA described up to now, with sizes varying in the range 250-2500 kb (compared with the 16 kb of mammalian mitochondria) [l]. The greater size of plant mitochondrial DNA may be taken as an indication of the presence of additional genes that are not found in the organelles from other species. Indeed, several ribosomal protein genes and the atpA gene coding for the a subunit of the H+-ATPase [2] have been found in plant mitochondrial genomes in contrast with other mitochondrial genomes. Most of the identified plant mitochondrial genes have counterparts in animal and yeast mitochondrial genomes. However, the recent determination of the Marchantia polymorpha mtDNA sequence [3] has shown that plant mitochondrial genomes contain a number of unidentified open reading frames which may correspond to either nuclear-encoded proteins in animal and fungi or to plant-specific polypeptides.Plant mitochondria seem to express and modulate the genetic information in a relatively complex way. Recently, editing [4-71 and trans-splicing [8-111 of plant mitochondrial mRNA have been described. Editing involves all plant mitoCorrespondence to J

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The path to RNA editing in plant mitochondria: The Halifax chapter

Gray

2009

IUBMB Life

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SummaryA single C/T difference between gene and cDNA within the 5 0 -untranslated region of the wheat mtDNA-encoded cox2 (cytochrome oxidase subunit 2) sequence catalyzed the discovery of C-to-U RNA editing in plant mitochondria by a group at Dalhousie University in Halifax, Nova Scotia [Covello and Gray, (1989) Nature 341, 662-666]. Editing of codons specifying essential copper-binding ligands in the Cox2p Cu A site provided additional insights about the amino acid residues comprising this site, as well as illustrating the functional importance of editing in wheat mitochondria. Further investigations of RNA editing in plant mitochondria led to the subsequent discovery of a case of mitochondrion-to-nucleus gene (cox2) transfer in soybean, mediated by reverse transcription of edited cox2 mRNA.

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The wheat cytochrome oxidase subunit II gene has an intron insert and three radical amino acid changes relative to maize

Cited by 154 publications

References 28 publications

Cytochrome Oxidase Subunit II Gene from Carrot Contains an Intron

Cytochrome Oxidase Subunit II Gene from Carrot Contains an Intron

Higher plant mitochondria encode an homologue of the nuclear‐encoded 30‐kDa subunit of bovine mitochondrial complex I

The path to RNA editing in plant mitochondria: The Halifax chapter

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