The Western Dietary Pattern Combined with Vancomycin-Mediated Changes to the Gut Microbiome Exacerbates Colitis Severity and Colon Tumorigenesis

Aardema, Niklas; Rodriguez, Daphne; Wettere, Arnaud J. Van; Benninghoff, Abby D.; Hintze, Korry

doi:10.3390/nu13030881

Cited by 9 publications

(7 citation statements)

References 71 publications

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“…As observed repeatedly in this murine model of CAC [ 9 , 22 , 32 ], mice fed the TWD (irrespective of FMT source) had shorter colon length, more severe colitis symptoms, higher inflammation and mucosa injury scores, higher tumor multiplicity, larger average tumor volume, and higher tumor burden compared to their AIN-fed counterparts. Interestingly, in this experiment, we also observed a marked increase in colon tumor incidence in the TWD-fed recipient mice.…”

Section: Discussionsupporting

confidence: 58%

Basal Diet Fed to Recipient Mice Was the Driving Factor for Colitis and Colon Tumorigenesis, despite Fecal Microbiota Transfer from Mice with Severe or Mild Disease

et al. 2023

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Consumption of the total Western diet (TWD) in mice has been shown to increase gut inflammation, promote colon tumorigenesis, and alter fecal microbiome composition when compared to mice fed a healthy diet, i.e., AIN93G (AIN). However, it is unclear whether the gut microbiome contributes directly to colitis-associated CRC in this model. The objective of this study was to determine whether dynamic fecal microbiota transfer (FMT) from donor mice fed either the AIN basal diet or the TWD would alter colitis symptoms or colitis-associated CRC in recipient mice, which were fed either the AIN diet or the TWD, using a 2 × 2 factorial experiment design. Time-matched FMT from the donor mice fed the TWD did not significantly enhance symptoms of colitis, colon epithelial inflammation, mucosal injury, or colon tumor burden in the recipient mice fed the AIN diet. Conversely, FMT from the AIN-fed donors did not impart a protective effect on the recipient mice fed the TWD. Likewise, the composition of fecal microbiomes of the recipient mice was also affected to a much greater extent by the diet they consumed than by the source of FMT. In summary, FMT from the donor mice fed either basal diet with differing colitis or tumor outcomes did not shift colitis symptoms or colon tumorigenesis in the recipient mice, regardless of the basal diet they consumed. These observations suggest that the gut microbiome may not contribute directly to the development of disease in this animal model.

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Section: Discussionsupporting

confidence: 58%

Basal Diet Fed to Recipient Mice Was the Driving Factor for Colitis and Colon Tumorigenesis, despite Fecal Microbiota Transfer from Mice with Severe or Mild Disease

et al. 2023

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“…Consumption of a rodent diet that emulates the typical American pattern with respect to macro-and micronutrient profiles has been repeatedly shown to enhance symptoms of colitis, inflammatory cell infiltration, mucosal injury and tumor development in murine models of colitis-associated colorectal cancer [12,35]. Herein, we report the findings from the first investigation to explore the purported benefit of dietary supplementation with BRB for gut health and modulation of the gut microbiome in a mouse model of Westerndiet enhanced colitis and colitis-associated colorectal cancer.…”

Section: Discussionmentioning

confidence: 99%

“…Mice were housed in a specific pathogen-free vivarium in the Laboratory Animal Research Center (LARC) at Utah State University, an AAALAC-approved facility. To be consistent with previous work on the role of the TWD in CAC [12,13,35,36], male C57BL/6J mice were obtained from Jackson Laboratories (Bar Harbor, ME) at five weeks of age. Mice were group-housed in sterile microisolator cages with Bed-o'Cobs ® 1 /4 bedding (Andersons, Cincinnati, OH, USA) supplied with HEPA-filtered microisolator cages in an IVC Air Handling Solutions ventilated housing system (Tecniplast, Buguggiate, Italy).…”

Section: Animals and Experimental Dietsmentioning

confidence: 99%

Dietary Supplementation with Black Raspberries Altered the Gut Microbiome Composition in a Mouse Model of Colitis-Associated Colorectal Cancer, although with Differing Effects for a Healthy versus a Western Basal Diet

et al. 2022

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Black raspberries (BRB) are rich in anthocyanins with purported anti-inflammatory properties. However, it is not known whether dietary supplementation would ameliorate Western-diet enhanced gut inflammation and colon tumorigenesis. We employed a mouse model of colitis-associated colorectal cancer (CAC) to determine the effects of dietary supplementation with 5 to 10% (w/w) whole, freeze-dried BRB in male C57BL/6J mice fed either a standard healthy diet (AIN93G) or the total Western diet (TWD). In a pilot study, BRB suppressed colitis and colon tumorigenesis while also shifting the composition of the fecal microbiome in favor of taxa with purported health benefits, including Bifidobacterium pseudolongum. In a follow-up experiment using a 2 ´ 2 factorial design with AIN and TWD basal diets with and without 10% (w/w) BRB, supplementation with BRB reduced tumor multiplicity and increased colon length, irrespective of the basal diet, but it did not apparently affect colitis symptoms, colon inflammation or mucosal injury based on histopathological findings. However, BRB intake increased alpha diversity, altered beta diversity and changed the relative abundance of Erysipelotrichaceae, Bifidobacteriaceae, Streptococcaceae, Rikenellaceae, Ruminococcaceae and Akkermansiaceae, among others, of the fecal microbiome. Notably, changes in microbiome profiles were inconsistent with respect to the basal diet consumed. Overall, these studies provide equivocal evidence for in vivo anti-inflammatory effects of BRB on colitis and colon tumorigenesis; yet, BRB supplementation led to dynamic changes in the fecal microbiome composition over the course of disease development.

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“…Antibiotics have proven their ability to inhibit the development of colitis [ 45 , 46 ]. Experimentally, oral vancomycin is used to evaluate changes during chronic disease models due to the lack of systemic absorption and its localized effects on the gut [ 47 ]. In this study, after seven days of treatment, our results indicated that oral vancomycin treatment showed higher efficiency than FMT and LBP freeze-dried powder.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of microbial and vancomycin treatments in ulcerative colitis in murine models

Hasan

Yang

2023

PLoS ONE

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Background Despite the number of available therapies for ulcerative colitis (UC), severe side effects and high cost has limited their clinical application. Thus, finding new alternative strategies with minimal side effects is inevitable. Therefore, this study aimed to compare the effectiveness of different therapeutic approaches in DSS-induced colitis. Methods Firstly, we designed oral bio-therapeutic products, Live Bacterial Products (LBP), which include a mixture of fecal bacteria strains isolated from healthy mice and prepared by microencapsulation and freeze-dried techniques. Then we investigated the efficiency of 7 days of freeze-dried FMT, LBP, and vancomycin treatments in DSS-induced colitis. Secondly, we compared the effect of 15 days of microbial therapies (freeze-dried powder of FMT and LBP microcapsules) and seven days of oral vancomycin on the severity of colitis in mice. Furthermore, the levels of IL-1β and TNF-α were measured in serum by ELISA, and the fecal microbiota diversity was analyzed by high-throughput sequencing for all mice groups. Results After seven days of treatments, our results indicated that oral vancomycin reduced the severity of DSS-induced colitis in mice, where weight gain and a decrease in IL-1 β and TNF-α levels were observed in the vancomycin group compared with other treatment groups. While after two weeks of treatment, the LBP microcapsules were able to reduce the severity of colitis. And at the end of the treatment period, weight gain and a decrease in the DAI scores and the levels of IL-1β and TNF-α were noted in the LBP treatment group compared to other treatment groups. By high-throughput sequencing of the 16S rRNA gene, our results showed that while the microcapsules LBP treatment increased the fecal microbial diversity, after vancomycin therapy, most of the fecal microbiota genera and operational taxonomic units (OTUs) were depleted. Conclusion Our results concluded that treatment duration and preparation methods affect the microbial therapies’ efficiency in UC. Furthermore, this study highlighted the negative consequences of oral vancomycin administration on gut health that should be known before using this medication.

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The Western Dietary Pattern Combined with Vancomycin-Mediated Changes to the Gut Microbiome Exacerbates Colitis Severity and Colon Tumorigenesis

Cited by 9 publications

References 71 publications

Basal Diet Fed to Recipient Mice Was the Driving Factor for Colitis and Colon Tumorigenesis, despite Fecal Microbiota Transfer from Mice with Severe or Mild Disease

Basal Diet Fed to Recipient Mice Was the Driving Factor for Colitis and Colon Tumorigenesis, despite Fecal Microbiota Transfer from Mice with Severe or Mild Disease

Dietary Supplementation with Black Raspberries Altered the Gut Microbiome Composition in a Mouse Model of Colitis-Associated Colorectal Cancer, although with Differing Effects for a Healthy versus a Western Basal Diet

Evaluation of microbial and vancomycin treatments in ulcerative colitis in murine models

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