The way for standardization on FDP/D dimer

勝幸, 福武

doi:10.2491/jjsth.27.653

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…The natural human D-dimer is a dimer, the dimeric structure of which is held by two covalent, intermolecular isopeptide bonds between the γ chains. 2–4 Therefore, the obtained antigen was identified using nonreducing SDS-PAGE, reducing SDS-PAGE, Western blotting, and indirect ELISA. D-dimer antigen purchased from Abcam served as the control, and antibody DD 1D10 was used as the primary antibody.…”

Section: Resultsmentioning

confidence: 99%

“…The dimeric structure of D-dimer is held by two covalent, intermolecular isopeptide bonds between the γ chains. 2–4 The concentration of plasma D-dimer changes drastically during fibrin production; therefore, it is an ideal blood-native biomarker of coagulation and fibrinolysis and may be used as a measure of normality or abnormality of the coagulation system. 5 In general, <0.5 μg/mL of D-dimer is detectable in healthy individuals.…”

Section: Introductionmentioning

confidence: 99%

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Generation of a Monoclonal Antibody against D-Dimer Using HTS-Based LiCA

et al. 2020

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D-dimer is an essential diagnostic index of thrombotic diseases. Since the existing anti-D-dimer antibodies vary in quality and specificity, a search for alternative anti-D-dimer antibodies is required. The present study aimed to screen a novel monoclonal antibody (mAb) against D-dimer using a light-initiated chemiluminescence assay (LiCA). In this work, mice were immunized with antigen prepared from human plasma by enzyme hydrolysis. After screening, a novel mAb, DD 2G11, was obtained. The results of sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis indicated that DD 2G11 could be used as a standard marker for D-dimer. The isotype of DD 2G11 was IgG1, the Ka value was 0.646 nM-1, and the Kd value was 50 nM, indicating that the binding affinity to D-dimer was very high. Furthermore, no cross-reactivity between DD 2G11 and other fibrinogen degradation products (FgDPs) was found. Finally, the correlation between DD 2G11 and the reference antibody (commercial antibody) was investigated by analyzing 56 clinical samples using a latex-enhanced turbidimetric immunoassay (LTIA). The R2 value of the linear regression was 0.94538, indicating that DD 2G11 met clinical requirements. In conclusion, the present study provides a more expeditious protocol to screen mAbs and provides a clinically usable mAb against D-dimer.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Generation of a Monoclonal Antibody against D-Dimer Using HTS-Based LiCA

et al. 2020

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“…The D-dimer values, which peak at 3 h after injury and then decline [ 8 ], may be underestimated or overestimated depending on the timing. Finally, a disparity in the calculation of the D-dimer value may exist between facilities depending on different test reagents used [ 18 ]. The Tokyo Clinical Laboratory Engineers Association [ 19 ] reported that the disparity in experiments using the same sample and the top three reagents, which account for 90% of the usage in Tokyo, was within ± 15% of the average value.…”

Section: Discussionmentioning

confidence: 99%

Serum D-dimer level as a predictor of neurological functional prognosis in cases of head injuries caused by road traffic accidents

et al. 2022

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Background The number of traffic fatalities is declining in Japan; however, a large proportion of head injuries are still attributable to traffic accidents. Severe head trauma may cause progressive and devastating coagulopathy owing to exacerbated coagulation and fibrinolysis, which results in massive bleeding and poor patient outcomes. D-dimer is a fibrinolytic marker, which remarkably increases in severe coagulopathy due to the exacerbated fibrinolytic system. Because the degree of coagulopathy is associated with patient outcomes, the D-dimer level is a useful prognostic predictor in patients with head trauma. However, the usefulness of D-dimer in cases of head trauma caused by road traffic accidents remains inadequately explored. In this study, we investigated the relationship between D-dimer levels and outcomes in head injuries caused by traffic accidents. Methods We extracted data on traffic injuries from Japan Neuro-Trauma Data Bank Project 2015, which is a prospective multicenter registry of head injuries. The analysis included 335 individuals with no missing data. The outcome variable was the score of the Glasgow Outcome Scale (GOS), a neurological outcome index. The participants were categorized into the favorable outcome (GOS score ≥ 4) and poor outcome (GOS score ≤ 3) groups. The serum D-dimer levels at the time of admission were divided into four categories at the quartiles, and the reference category was less than the first quartile (< 17.4 µg/mL). We performed a logistic regression analysis with GOS as the dependent variable and D-dimer as a predictor and performed a multivariate analysis that was adjusted for 10 physiological parameters. Results In the univariate analysis, all groups with serum D-dimer values ≥ 17.4 μg/dL showed significantly poorer outcomes than those of the reference group. In the multivariate analysis, after adjusting for other factors, D-dimer levels ≥ 89.3 μg/dL were an independent predictor of poor outcome. Conclusion After adjusting for physiological parameters, high serum D-dimer levels can be an independent factor for predicting neurological prognosis in head trauma caused by road traffic accidents.

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The Association Between D-dimer Levels and Long-Term Neurological Outcomes of Patients with Traumatic Brain Injury: An Analysis of a Nationwide Observational Neurotrauma Database in Japan

et al. 2021

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The full-text file will be made open to the public on 30 August 2022 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.; This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 Title: Associations between initial serum pH value and outcomes of pediatric out-of-hospital cardiac arrest ABSTRACT Introduction: Pediatric out-of-hospital cardiac arrest (OHCA) is one of the most critical conditions seen in the emergency department (ED). Although initial serum pH value is reported to be associated with outcome in adult OHCA patients, the association is unclear in pediatric OHCA patients. Thus, we aimed to identify the association between initial pH value and outcome among pediatric OHCA patients.Methods: This study was a retrospective analysis of a multicenter prospective cohort registry (Japanese Association for Acute Medicine out-of-hospital cardiac arrest registry) from 87 hospitals in Japan. We included pediatric OHCA patients younger than 16 years of age who were registered in this registry between June 2014 and December 2017. Of the 34,754 patients in the database, 458 patients were ultimately included in the analysis. We equally divided the patients into four groups, based on their initial pH value, and conducted a multivariate logistic regression analysis to calculate the adjusted odds ratios of the initial pH value on hospital arrival with their 95% confidence intervals for the primary outcome. Results:The median (interquartile range) age was 1 (0-6) year, and 77.9% (357/458) of the first monitored rhythm was asystole. The primary outcome was 1-month survival. The overall 1-month survival was 13.3% (61/458), and a 1-month favorable neurologic outcome was seen in 5.2% (24/458) of cases. The adjusted odds ratios and 95% confidence intervals for the pH 6. 81-6.64, pH 6.63-6.47, pH <6.47, and pH unknown groups compared with the pH ≥6.82 group for 1-month survival were 0.

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The way for standardization on FDP/D dimer

Cited by 3 publications

References 5 publications

Generation of a Monoclonal Antibody against D-Dimer Using HTS-Based LiCA

Generation of a Monoclonal Antibody against D-Dimer Using HTS-Based LiCA

Serum D-dimer level as a predictor of neurological functional prognosis in cases of head injuries caused by road traffic accidents

The Association Between D-dimer Levels and Long-Term Neurological Outcomes of Patients with Traumatic Brain Injury: An Analysis of a Nationwide Observational Neurotrauma Database in Japan

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