The waterlogging/salinity interaction in higher plants revisited – focusing on the hypoxia-induced disturbance to K+ homeostasis

Barrett-Lennard, E.G.; Shabala, Sergey

doi:10.1071/fp12235

Cited by 89 publications

(64 citation statements)

References 68 publications

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“…This is the first investigation of cell-specific ion distributions across roots in relation to radial O 2 concentrations. So far, ion transport processes in O 2 -deficient roots have been considered in short-term experiments on maize or barley seedlings (Drew & L€ auchli, 1985;Gibbs et al, 1998;Pang et al, 2006;Zeng et al, 2014) or speculated upon in reviews (Colmer & Greenway, 2011;Barrett-Lennard & Shabala, 2013). Barley was chosen as it is a relatively salt-tolerant cereal (James et al, 2006) but it is sensitive to waterlogging (Garthwaite et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Oxygen deficiency and salinity affect cell‐specific ion concentrations in adventitious roots of barley (Hordeum vulgare)

et al. 2015

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Summary Oxygen deficiency associated with soil waterlogging adversely impacts root respiration and nutrient acquisition. We investigated the effects of O2 deficiency and salinity (100 mM NaCl) on radial O2 concentrations and cell‐specific ion distributions in adventitious roots of barley (Hordeum vulgare). Microelectrode profiling measured O2 concentrations across roots in aerated, aerated saline, stagnant or stagnant saline media. X‐ray microanalysis at two positions behind the apex determined the cell‐specific elemental concentrations of potassium (K), sodium (Na) and chloride (Cl) across roots. Severe O2 deficiency occurred in the stele and apical regions of roots in stagnant solutions. O2 deficiency in the stele reduced the concentrations of K, Na and Cl in the pericycle and xylem parenchyma cells at the subapical region. Near the root apex, Na declined across the cortex in roots from the aerated saline solution but was relatively high in all cell types in roots from the stagnant saline solution. Oxygen deficiency has a substantial impact on cellular ion concentrations in roots. Both pericycle and xylem parenchyma cells are involved in energy‐dependent K loading into the xylem and in controlling radial Na and Cl transport. At root tips, accumulation of Na in the outer cell layers likely contributed to reduction of Na in inner cells of the tips.

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Section: Introductionmentioning

confidence: 99%

Oxygen deficiency and salinity affect cell‐specific ion concentrations in adventitious roots of barley (Hordeum vulgare)

et al. 2015

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“…). Saltwater flooding increases the Na + and Cl − concentrations in shoots, leaves and roots and causes severe growth and developmental defects (Barrett‐Lennard & Shabala ). Nevertheless, it is unclear how plant stress adaptation mechanisms are compounded in such complex conditions.…”

Section: Introductionmentioning

confidence: 99%

Inverse modulation of the energy sensor Snf1‐related protein kinase 1 on hypoxia adaptation and salt stress tolerance in Arabidopsis thaliana

Cho

Kim

et al. 2014

Plant Cell & Environment

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Terrestrial plants are exposed to complex stresses of high salt-induced abscisic acid (ABA) and submergence-induced hypoxia when seawater floods fields. Many studies have investigated plant responses to individual stress conditions, but not so much for coupled or sequentially imposed stresses. We examined molecular regulatory mechanisms of gene expression underlying the cellular responses involved in crosstalk between salt and hypoxia stresses. Salt/ABA- and AtMYC2-dependent induction of a synthetic ABA-responsive element and the native RD22 promoters were utilized in our cell-based functional assays. Such promoter-based reporter induction was largely inhibited by hypoxia and hypoxia-inducible AKIN10 activity. Biochemical analyses showed that AKIN10 negatively modulates AtMYC2 protein accumulation via proteasome activity upon AKIN10 kinase activity-dependent protein modification. Further genetic analysis using transgenic plants expressing AKIN10 provided evidence that AKIN10 activity undermined AtMYC2-dependent salt tolerance. Our findings unravel a novel molecular interaction between the key signalling constituents leading crosstalk between salt and hypoxia stresses in Arabidopsis thaliana under the detrimental condition of submergence in saltwater.

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“…Although the central role of intracellular K + homeostasis in plant‐adaptive responses to flooding has been highlighted (Mugnai et al . ; Barrett‐Lennard & Shabala ; Shabala et al . ), reported experimental results remain controversial.…”

Section: Introductionmentioning

confidence: 99%

Linking oxygen availability with membrane potential maintenance and K⁺ retention of barley roots: implications for waterlogging stress tolerance

Zeng

Konnerup

Shabala

et al. 2014

Plant Cell & Environment

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Oxygen deprivation is a key determinant of root growth and functioning under waterlogging. In this work, changes in net K(+) flux and membrane potential (MP) of root cells were measured from elongation and mature zones of two barley varieties under hypoxia and anoxia conditions in the medium, and as influenced by ability to transport O2 from the shoot. We show that O2 deprivation results in an immediate K(+) loss from roots, in a tissue- and time-specific manner, affecting root K(+) homeostasis. Both anoxia and hypoxia induced transient membrane depolarization; the extent of this depolarization varied depending on severity of O2 stress and was less pronounced in a waterlogging-tolerant variety. Intact roots of barley were capable of maintaining H(+) -pumping activity under hypoxic conditions while disrupting O2 transport from shoot to root resulted in more pronounced membrane depolarization under O2 -limited conditions and in anoxia a rapid loss of the cell viability. It is concluded that the ability of root cells to maintain MP and cytosolic K(+) homeostasis is central to plant performance under waterlogging, and efficient O2 transport from the shoot may enable operation of the plasma membrane H(+) -ATPase in roots even under conditions of severe O2 limitation in the soil solution.

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The waterlogging/salinity interaction in higher plants revisited – focusing on the hypoxia-induced disturbance to K+ homeostasis

Cited by 89 publications

References 68 publications

Oxygen deficiency and salinity affect cell‐specific ion concentrations in adventitious roots of barley (Hordeum vulgare)

Oxygen deficiency and salinity affect cell‐specific ion concentrations in adventitious roots of barley (Hordeum vulgare)

Inverse modulation of the energy sensor Snf1‐related protein kinase 1 on hypoxia adaptation and salt stress tolerance in Arabidopsis thaliana

Linking oxygen availability with membrane potential maintenance and K⁺ retention of barley roots: implications for waterlogging stress tolerance

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The waterlogging/salinity interaction in higher plants revisited – focusing on the hypoxia-induced disturbance to K+ homeostasis

Cited by 89 publications

References 68 publications

Oxygen deficiency and salinity affect cell‐specific ion concentrations in adventitious roots of barley (Hordeum vulgare)

Oxygen deficiency and salinity affect cell‐specific ion concentrations in adventitious roots of barley (Hordeum vulgare)

Inverse modulation of the energy sensor Snf1‐related protein kinase 1 on hypoxia adaptation and salt stress tolerance in Arabidopsis thaliana

Linking oxygen availability with membrane potential maintenance and K+ retention of barley roots: implications for waterlogging stress tolerance

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Linking oxygen availability with membrane potential maintenance and K⁺ retention of barley roots: implications for waterlogging stress tolerance