The wastewater protist Rhogostoma minus (Thecofilosea, Rhizaria) is abundant, widespread, and hosts Legionellales

Pohl, Nina; Solbach, Marcel Dominik; Dumack, Kenneth

doi:10.1016/j.watres.2021.117566

Cited by 14 publications

(3 citation statements)

References 73 publications

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“…The Rhogostoma genus increased in abundance, dominating the community by the end of the floccular stage. They have been observed as dominating eukaryotic communities in several WWTPs (Chouari et al, 2017; Hirakata et al, 2019; Matsunaga et al, 2014; Remmas et al, 2017) and found to be represented by a single species, Rhogostoma minus, which recently have received researchers attention not only for its wide spread distribution but for hosting well-known human pathogenic Legionellales (Pohl et al, 2021). The abundance of the Sessilida family ( Alveolata supergroup) in turn reached a minimum at the end of the floccular stage.…”

Section: Discussionmentioning

confidence: 99%

Community successional patterns and inter-kingdom interactions during granular biofilm development

de Celis,

Modin,

Arregui

et al. 2024

Preprint

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Aerobic granular sludge is a compact and efficient biofilm process used for wastewater treatment which has received much attention and is currently being implemented worldwide. The microbial associations and their ecological implications occurring during granule development, especially those involving inter-kingdom interactions, are poorly understood. In this work, we monitored the prokaryote and eukaryote community composition and structure during the granulation of activated sludge for 343 days in a sequencing batch reactor (SBR), and investigated the influence of abiotic and biotic factors on the granule development. Sludge granulation was accomplished with low-wash out dynamics at long settling times, allowing for the microbial communities to adapt to the SBR environmental conditions. The sludge granulation and associated changes in microbial community structure could be divided into three stages: floccular, intermediate, and granular. The eukaryotic and prokaryotic communities showed parallel successional dynamics, with three main sub-communities identified for each kingdom, dominating in each stage of sludge granulation. Although inter-kingdom interactions were shown to affect community succession during the whole experiment, during granule development random factors like the availability of settlement sites or drift acquired increasing importance. The prokaryotic community was more affected by deterministic factors, including reactor conditions, while the eukaryotic community was to a larger extent shaped by biotic interactions (including inter-kingdom interactions) and stochasticity.

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Section: Discussionmentioning

confidence: 99%

Community successional patterns and inter-kingdom interactions during granular biofilm development

de Celis,

Modin,

Arregui

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…We argue that this shaping force is largely caused by protistan predation. All of the most numerous protists in this study are known bacterivores (Böhme et al, 2009;Vaerewijck et al, 2011;Samba-Louaka et al, 2019), including sessile peritrich ciliates like Vorticella, which are considered important for treatment efficiency, effluent clarity, and nitrification, as well as Kinetoplastida and Cryomonadida, both of which are known to feed on wastewater bacteria (Pauli et al, 2001;Arregui et al, 2012;Foissner, 2016;Liu et al, 2016;Pohl et al, 2021;Freudenthal et al, 2022). The most abundant protistan taxon in this study, bacterivorous Vannelida, is often absent from wastewater surveys that rely on primer-based methods, but studies based on culturing or metatranscriptomic methods have repeatedly reported their presence in WWTP bioreactors (Ramirez et al, 2015;Freudenthal et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Microeukaryotic predators shape the wastewater microbiome

2023

Self Cite

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“…Most of these findings documented the involvement of amoebozoans and heteroloboseans representative in such interactions (Balczun and Scheid, 2017;Shi et al, 2021). However, recent work has demonstrated that numerous rhizarian FLA could also bear intracellular bacteria, thereby confirming that tight FLA-bacteria interactions indeed occur in all these taxonomic groups (Pohl et al, 2021;Solbach et al, 2021).…”

Section: Rosculus Vilicus Interacts With Map For At Least a Few Daysmentioning

confidence: 92%

Characterization of Rosculus vilicus sp. nov., a rhizarian amoeba interacting with Mycobacterium avium subsp. paratuberculosis

Jessu,

Delafont,

Moyen

et al. 2023

Front. Microbiol.

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Free-living amoebae are described as potential reservoirs for pathogenic bacteria in the environment. It has been hypothesized that this might be the case for Mycobacterium avium subsp. paratuberculosis, the bacterium responsible for paratuberculosis. In a previous work, we isolated an amoeba from a water sample in the environment of infected cattle and showed that this amoeba was associated with Mycobacterium avium subsp. paratuberculosis. While a partial 18S rRNA gene has allowed us to suggest that this amoeba was Rosculus-like, at that time we were not able to sub-cultivate it. In the present study, we succeeded in cultivating this strain at 20–25°C. This amoeba is among the smallest (5–7 μm) described. The sequencing of the whole genome allowed us to extract the full 18S rRNA gene and propose this strain as a new species of the Rosculus genus, i.e., R. vilicus. Of note, the mitochondrial genome is particularly large (184,954 bp). Finally, we showed that this amoeba was able to phagocyte Mycobacterium avium subsp. paratuberculosis and that the bacterium was still observed within amoebae after at least 3 days. In conclusion, we characterized a new environmental amoeba species at the cellular and genome level that was able to interact with Mycobacterium avium subsp. paratuberculosis. As a result, R. vilicus is a potential candidate as environmental reservoir for Mycobacterium avium subsp. paratuberculosis but further experiments are needed to test this hypothesis.

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The wastewater protist Rhogostoma minus (Thecofilosea, Rhizaria) is abundant, widespread, and hosts Legionellales

Cited by 14 publications

References 73 publications

Community successional patterns and inter-kingdom interactions during granular biofilm development

Community successional patterns and inter-kingdom interactions during granular biofilm development

Microeukaryotic predators shape the wastewater microbiome

Characterization of Rosculus vilicus sp. nov., a rhizarian amoeba interacting with Mycobacterium avium subsp. paratuberculosis

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