The W303 genetic background affects theisw2Δ mutant phenotype inSaccharomyces cerevisiae

Trachtulcová, Petra; Frýdlová, Ivana; Janatová, Ivana; Dorosh, Andriy; Hašek, Jir̆ı́

doi:10.1007/bf02931508

Cited by 8 publications

(4 citation statements)

References 22 publications

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“…Indeed, W303 is a widely used strain background in the yeast community, but it has amino acid alterations in 799 proteins, including factors of ageing and stress resistance, when compared to S288C, from which BY4741 was derived [ 181 ]. Significant differences between responses in these 2 genetic backgrounds have already been described [ 182 ]. It should be noted that in W303, most of Not4 was observed in polysomes [ 180 ], whereas only some Not4 was observed in polysomes in the BY4741 background [ 138 ].…”

Section: Not4 a New Player In Cotranslational Quality Controlmentioning

confidence: 99%

The Not4 RING E3 Ligase: A Relevant Player in Cotranslational Quality Control

Collart

2013

ISRN Molecular Biology

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The Not4 RING E3 ligase is a subunit of the evolutionarily conserved Ccr4-Not complex. Originally identified in yeast by mutations that increase transcription, it was subsequently defined as an ubiquitin ligase. Substrates for this ligase were characterized in yeast and in metazoans. Interestingly, some substrates for this ligase are targeted for polyubiquitination and degradation, while others instead are stable monoubiquitinated proteins. The former are mostly involved in transcription, while the latter are a ribosomal protein and a ribosome-associated chaperone. Consistently, Not4 and all other subunits of the Ccr4-Not complex are present in translating ribosomes. An important function for Not4 in cotranslational quality control has emerged. In the absence of Not4, the total level of polysomes is reduced. In addition, translationally arrested polypeptides, aggregated proteins, and polyubiquitinated proteins accumulate. Its role in quality control is likely to be related on one hand to its importance for the functional assembly of the proteasome and on the other hand to its association with the RNA degradation machines. Not4 is in an ideal position to signal to degradation mRNAs whose translation has been aborted, and this defines Not4 as a key player in the quality control of newly synthesized proteins.

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Section: Not4 a New Player In Cotranslational Quality Controlmentioning

confidence: 99%

The Not4 RING E3 Ligase: A Relevant Player in Cotranslational Quality Control

Collart

2013

ISRN Molecular Biology

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“…The absence of either Isw2 or Itc1 in MAT␣ cells results in an inappropriate a-factor production and an autocrine activation of the pheromone response pathway. As a result, the pheromone-inducible genes are upregulated and cells display altered morphology resembling budding "shmoos" (20,28,30,31). However, the level of derepression of MATa-specific genes in isw2⌬ MAT␣ cells as a direct effect of the Isw2 absence is very low.…”

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confidence: 99%

Deregulation of DSE1 Gene Expression Results in Aberrant Budding within the Birth Scar and Cell Wall Integrity Pathway Activation in Saccharomyces cerevisiae

et al. 2009

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Strains of Saccharomyces cerevisiae lacking Isw2, the catalytic subunit of the Isw2 chromatin remodeling complex, show the mating type-independent activation of the cell wall integrity (CWI) signaling pathway. Since the CWI pathway activation usually reflects cell wall defects, we searched for the cell wall-related genes changed in expression. The genes DSE1, CTS1, and CHS1 were upregulated as a result of the absence of Isw2, according to previously published gene expression profiles (I. Frydlova, M. Basler, P. Vasicova, I. Malcova, and J. Hasek, Curr. Genet. 52:87-95, 2007). Western blot analyses of double deletion mutants, however, did not indicate the contribution of the chitin metabolism-related genes CTS1 and CHS1 to the CWI pathway activation. Nevertheless, the deletion of the DSE1 gene encoding a daughter cell-specific protein with unknown function suppressed CWI pathway activation in isw2⌬ cells. In addition, the deletion of DSE1 also abolished the budding-within-the-birth-scar phenotype of isw2⌬ cells. The plasmid-driven overexpression proved that the deregulation of Dse1 synthesis was also responsible for CWI pathway activation and manifestation of the budding-within-the-birth-scar phenotype in wild-type cells. The overproduced Dse1-green fluorescent protein localized to both sides of the septum and persisted in unbudded cells. Although the exact cellular role of this daughter cell-specific protein has to be elucidated, our data point to the involvement of Dse1 in bud site selection in haploid cells.

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“…, 2020 ). Finally, cell wall instability is an elegant explanation for the observation that rrp6Δ phenotype, that is, its temperature sensitivity, is most pronounced in W303 and its derived genetic backgrounds, as wild type of this strain was shown to have an already more destabilized cell wall compared with wild types of other backgrounds ( Trachtulcová et al. , 2003 ; Schroeder and Ikui, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Yeast RNA exosome activity is necessary for maintaining cell wall stability through proper protein glycosylation

Novačić

Beauvais

Oskomić

et al. 2021

MBoC

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Nuclear RNA exosome is the main 3'→5' RNA degradation and processing complex in eukaryotic cells and its dysregulation therefore impacts gene expression and viability. In this work we show that RNA exosome activity is necessary for maintaining cell wall stability in yeast Saccharomyces cerevisiae. While the essential RNA exosome catalytic subunit Dis3 provides exoribonuclease catalytic activity, the second catalytic subunit Rrp6 has a non-catalytic role in this process. RNA exosome cofactors Rrp47 and Air1/2 are also involved. RNA exosome mutants undergo osmoremedial cell lysis at high temperature or at physiological temperature upon treatment with cell wall stressors. Finally, we show that a defect in protein glycosylation is a major reason for cell wall instability of RNA exosome mutants. Genes encoding enzymes that act in the early steps of the protein glycosylation pathway are downregulated at high temperature in cells lacking Rrp6 protein or Dis3 exoribonuclease activity and overexpression of the essential enzyme Psa1, that catalyzes synthesis of the mannosylation precursor, suppresses temperature sensitivity and aberrant morphology of these cells. Furthermore, this defect is connected to a temperature-dependent increase in accumulation of non-coding RNAs transcribed from loci encoding relevant glycosylation-related genes.

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The W303 genetic background affects theisw2Δ mutant phenotype inSaccharomyces cerevisiae

Cited by 8 publications

References 22 publications

The Not4 RING E3 Ligase: A Relevant Player in Cotranslational Quality Control

The Not4 RING E3 Ligase: A Relevant Player in Cotranslational Quality Control

Deregulation of DSE1 Gene Expression Results in Aberrant Budding within the Birth Scar and Cell Wall Integrity Pathway Activation in Saccharomyces cerevisiae

Yeast RNA exosome activity is necessary for maintaining cell wall stability through proper protein glycosylation

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