The Vroman effect: Competitive protein exchange with dynamic multilayer protein aggregates

It is now well accepted that cellular responses to materials in a biological medium reflect greatly the adsorbed biomolecular layer, rather than the material itself. Here, we study by molecular dynamic simulations the competitive protein adsorption on a surface (Vroman effect), i.e. the non-monotonic behavior of the amount of protein adsorbed on a surface in contact with plasma as a function of contact time and plasma concentration. We find a complex behavior, with regimes during which small and large proteins are not necessarily competing between them, but are both competing with others in solution. We show how the effect can be understood, controlled and inverted.

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“…However, the mechanisms of the phenomenon are still debated and no existing model can fully explain it 17,22,23 .…”

Section: Introductionmentioning

confidence: 99%

Understanding and modulating the competitive surface-adsorption of proteins through coarse-grained molecular dynamics simulations

2013

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“…Cell-based cytotoxicity tests of implantable MEAs ensure that the material used in the devices is biocompatible [181]. The dynamic replacement of proteins with the bigger ones on the bio-hardware interface (including neural MEAs) results in undesired layer instabilities that are difficult to control (Vroman effect) [182,183].Therefore, surface modification of the MEAs including polymerization and bioactive molecule-coating significantly improves the biocompatibility of neural implants in the vicinity of tissues and cells.…”

Section: Discussionmentioning

confidence: 99%

High-density 3D pyramid-shaped microelectrode arrays for brain-machine interface applications

Motlagh

2014

2014 IEEE Biomedical Circuits and Systems Conference (BioCAS) Proceedings

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“…Each of these materials is hydrophobic, with some being more hydrophobic than others depending on the side chain composition of the main polymer. The adsorptive process is also dynamic where proteins continually compete, adsorb, and desorb from the surface depending on both time and the changing concentrations of plasma [5]. Another important feature of this adsorptive process is that it often leads to a conformational change in the natural 3-D protein structure (Figure 2).…”

Section: Surface Activationmentioning

confidence: 99%

ECMO Biocompatibility: Surface Coatings, Anticoagulation, and Coagulation Monitoring

Maul¹,

Massicotte²,

Wearden³

2016

Extracorporeal Membrane Oxygenation: Advances in Therapy

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The interaction between the patient and the ECMO (extracorporeal membrane oxygenation) circuit initiates a significant coagulation and inflammatory response due to the large surface area of foreign material contained within the circuit. This response can be blunted with the appropriate mix of biocompatible materials and anticoagulation therapy. The use of anticoagulants, in turn, requires appropriate laboratory testing to determine whether the patient is appropriately anticoagulated. Physicians must balance the risks of bleeding with the risks of thrombosis; the proper interpretation of these tests is often shrouded in mystery. It is the purpose of this chapter to help demystify the coagulation system, anticoagulants, biocompatible surfaces, and coagulation testing so that ECMO practitioners can make informed decisions about their patients and to spur coordinated efforts for future research to improve our understanding of these complex processes.

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The Vroman effect: Competitive protein exchange with dynamic multilayer protein aggregates

Cited by 258 publications

References 48 publications

Understanding and modulating the competitive surface-adsorption of proteins through coarse-grained molecular dynamics simulations

Understanding and modulating the competitive surface-adsorption of proteins through coarse-grained molecular dynamics simulations

High-density 3D pyramid-shaped microelectrode arrays for brain-machine interface applications

ECMO Biocompatibility: Surface Coatings, Anticoagulation, and Coagulation Monitoring

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