The VPg of tobacco etch virus RNA is the 49-kDa proteinase or the N-terminal 24-kDa part of the proteinase

The Potato Leafroll Luteovirus 17K Protein is a Single-stranded Nucleic Acid-binding Protein

“…Suggestions have been made that this protein represents a precursor of the genome-linked VPg (van der Wilk et al, 1989). As multiple functions have been reported for nucleic acid-binding viral proteins (Citovsky et al, 1990;Murphy et al, 1990), the same might be true for prl7. By virtue of its ssRNA-binding capacity in the C-terminal domain and a putative dimerization domain at the N terminus, prl7 might protect PLRV RNA from degradation by cellular RNases and/or sequester genomic RNA to serve as a template in the replication complex.…”

VPg-mediated Aggregation of Potyviral RNA

“…Potyviral RNA contains a covalently linked protein (VPg) at the 5' terminus (Siaw et al, 1985;Riechmann et al, 1989;Murphy et al, 1990) and is polyadenylated at the 3' terminus (Hari et al, 1979). During electrophoretic analysis in agarose gels of the RNA of the potyvirus tobacco vein mottling virus (TVMV), some RNA migrated more slowly than the genomic RNA.…”

“…Burley 21 and purified by procedures described by Calvert &Ghabrial (1983) andMurphy et al (1990). Other potyviruses, tobacco etch virus (TEV) and potato virus Y (PVY), were purified from infected N. tabacum using Method 1 of Moghal & Francki (1976).…”

Role of the helper component in vector-specific transmission of potyviruses.

“…A highly aphidtransmissible strain of TEV (Pirone, 1981) was purified by the method of Murphy et al (1990). The aphid-transmissible isolate of turnip mosaic virus (TuMV) was that reported by Sako (1980) ; the virus was propagated in mustard and purified by a combination of the methods of Murphy et al (1990) and Sako (1980).…”

“…The aphid-transmissible isolate of turnip mosaic virus (TuMV) was that reported by Sako (1980) ; the virus was propagated in mustard and purified by a combination of the methods of Murphy et al (1990) and Sako (1980).…”