Many plants rely on insect pollination, yet numerous
agricultural
plant-breeding programs focus on traits that appeal to growers and
consumers instead of pollinators, leading to declining pollinator
attraction and crop yields. Using hybrid carrot seed production as
a model, we investigated low-yielding carrot varieties by analyzing
sugars and minerals in nectar and floral volatile composition. While
the analysis of nectar sugars and minerals did not reveal any key
differences between the carrot varieties, differences between the
112 detected volatiles in 23 samples were observed. Numerous differentiating
sesquiterpenes were identified in floral solvent extracts, and subsequent
behavioral assays showed that β-ocimene from higher-yielding
carrot varieties stimulated nectar feeding (attractant), while α-
and β-selinene from lower-yielding lines decreased feeding (deterrents).
Sesquiterpenes have previously been implicated in plant defense, suggesting
a trade-off between pollination and protection. Our results highlight
the importance of volatiles as regulators of pollinator attraction
in agricultural settings.