“…Department of Nutrition, Ontario Agricultural College, Guelph, Canada (Received 7 August 1962) The optimum pH for alkaline phosphatase depends on such factors as the source of enzyme and the type and concentration of substrate, buffers and activators (for references see Motzok & Branion, 1961). Also, the optimum pH of fowlintestinal phosphatase changes to more alkaline values with increasing age of the birds (Motzok & Branion, 1961). Although the pH optima for the various concentrations of glycerol 2-phosphate were quite constant for the intestinal enzyme of several breeds of chicks at 1 day of age, some differences have been observed among groups of older fowls of approximately the same age but given different experimental and commercial diets.…”