The vitamin-B12-producing bacteria in the water and sediment of a carp culture pond

Sugita, Haruo; Kuruma, Akiko; Hirato, C.; Ohkoshi, T.; Okada, Roberto Hirochi; Deguchi, Yoshiaki

doi:10.1016/0044-8486(94)90305-0

Cited by 10 publications

(8 citation statements)

References 11 publications

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“…Anaerobe in fish intestines then predominated with 10 5 -10 7 CFU g )1 (Sugita et al 1988). As this bacterium seems to be alive within 1-2 days after defecation (Sugita et al 1985), this bacterium often occurred in water and sediments of a carp culture pond (Sugita et al 1994). This fact strongly suggests that Bacteroides type A is a microaerotolerant anaerobe.…”

Section: Discussionmentioning

confidence: 95%

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Novel ecological niche of Cetobacterium somerae, an anaerobic bacterium in the intestinal tracts of freshwater fish

Tsuchiya

Sakata

Sugita

2007

Lett Appl Microbiol

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273

272

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Aims: This study was conducted to clarify the taxonomic status of Bacteroides type A strains with high vitamin B12‐producing ability that is widely distributed in the intestinal tracts of freshwater fish. Methods and Results: Seventeen strains of Bacteroides type A isolated from five fish species were all rod‐shaped and gram‐negative. The strains were positive for esculin hydrolysis, nitrate reduction, resistance to bile, acid phosphatase, and negative for the production of catalase and urease and the susceptibility to vancomycin. The G+C content of DNA from the 17 strains was 29·1–31·9 mol%, and 16S rDNA sequence analysis revealed a close phylogenetic relationship between Bacteroides type A strains and Cetobacterium somerae sharing 99·7–100% sequence similarity. In addition, strains were capable of producing vitamin B12 at a rate of 1·82–13·98 ng ml−1 in 48 h. Conclusion: Phenotypic and phylogenetic characteristics indicated that all isolates previously classified as Bacteroides type A strains belong to C. someare. Significance and Impact of the Study: This study provided the important finding of novel niche of vancomycin‐resistant bacteria such as C. somerae in the intestinal tract of freshwater fish.

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Section: Discussionmentioning

confidence: 95%

“…As this bacterium seems to be alive within 1–2 days after defecation (Sugita et al. 1985), this bacterium often occurred in water and sediments of a carp culture pond (Sugita et al. 1994).…”

Section: Discussionmentioning

confidence: 99%

Novel ecological niche of Cetobacterium somerae, an anaerobic bacterium in the intestinal tracts of freshwater fish

Tsuchiya

Sakata

Sugita

2007

Lett Appl Microbiol

Self Cite

273

272

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“…Cetobacterium (Fusobacteria) is known to produce vitamin B 12 (Sugita et al, 1994), as does Plesiomonas (Sugita et al, 1991). Planktonic Flavobacterium (phylum Bacteroidetes) isolated from eutrophic Lake Jeziorak (Poland) also produced several types of B vitamins (Donderski, 1991).…”

Section: Discussionmentioning

confidence: 99%

The epiphytic microbiota of the globally widespread macroalga Cladophora glomerata (Chlorophyta, Cladophorales)

Zulkifly

Hanshew

Young

et al. 2012

American J of Botany

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• Premise of the study: The filamentous chlorophyte Cladophora produces abundant nearshore populations in marine and freshwaters worldwide, often dominating periphyton communities and producing nuisance growths under eutrophic conditions. High surface area and environmental persistence foster such high functional and taxonomic diversity of epiphytic microfauna and microalgae that Cladophora has been labeled an ecological engineer. We tested the hypotheses that (1) Cladophora supports a structurally and functionally diverse epiphytic prokaryotic microbiota that influences materials cycling and (2) mutualistic host–microbe interactions occur. Because previous molecular sequencing‐based analyses of the microbiota of C. glomerata found as western Lake Michigan beach drift had identified pathogenic associates such as Escherichia coli, we also asked if actively growing lentic C. glomerata harbors known pathogens. • Methods: We used 16S rRNA gene amplicon pyrosequencing to examine the microbiota of C. glomerata of Lake Mendota, Dane, Wisconsin, United States, during the growing season of 2011, at the genus‐ or species‐level to infer functional phenotypes. We used correlative scanning electron and fluorescence microscopy to describe major prokaryotic morphotypes. • Key results: We found microscopic evidence for diverse bacterial morphotypes, and molecular evidence for ca. 100 distinct sequence types classifiable to genus at the 80% confidence level or species at the 96–97% level within nine bacterial phyla, but not E. coli or related human pathogens. • Conclusions: We inferred that bacterial epiphytes of lentic C. glomerata have diverse functions in materials cycling, with traits that indicate the occurrence of mutualistic interactions with the algal host.

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“…Isolation of Vitamin B12 Producing Bacteria was carried out by spread the sample water on selective PYBG agar media (trypticase peptone 1%, phytone peptone 0.5%, lab-lemco powder 0.24%, bacto yeast extract 0.2%, glucose 0.2%, agar 1%, pH 7.5) (Sugita et al 1994). The vitamin B12 concentration of the culture medium was assayed using the Salmonella typhimurium.…”

Section: Isolation and Screening Of Vitamin B12-producing Bacteriamentioning

confidence: 99%

Exploration of vitamin B12-producing bacteria from Indonesia Eutrophic Lake: A new strategy to improve microalgae biomass production

et al. 2021

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Abstract. Prabaningtyas S, Ardyati T, Suharjono, Retnaningdyah C. 2021. Exploration of vitamin B12-producing bacteria from Indonesia Eutrophic Lake: A new strategy to improve microalgae biomass production. Biodiversitas 22: 4538-4544. Biofuel producing autotrophs and heterotrophs organisms are mostly found in freshwaters such as lakes. Preliminary research showed that Ranu Grati has a high diversity of microalgae and bacteria. Microalgae are known as one source of biofuels due to the high content of lipid. The growth of microalgae is strongly influenced by vitamin B12, which is synthesized by bacteria. The role of vitamin B12 in the metabolism of algae is mainly as a co-enzyme for vitamin B12-dependent methionine synthase. The purpose of this research was to observe the bacterial diversity based on NGS analysis, to explore vitamin B12 produced by bacteria from Ranu Grati lake, and also to identify the highest potency of vitamin B12-producing bacteria based on 16S rRNA gene sequencing. The study was carried out by taking a water sample at a depth of 50 cm at five stations. Isolation of total bacterial DNA was carried out using FastDNA Spin Kit for Soil. The metagenomic method of Next Generation Sequencing (NGS) was used as an initial study of bacterial diversity. Vitamin B12-producing bacteria was isolated using PYBG agar (phytone peptone, trypticase peptone, lab-lemco powder, bacto yeast extract, glucose, agar). Isolated bacteria were screened for production of vitamin B12. The potential isolates were identified base on 16S rRNA gene sequence similarity. The results of the metagenomic study showed that the genus of potential bacteria producing vitamin B12 included Bacillus, Pseudomonas, Propionibacterium, Enterobacter, Escherichia, Acinetobacter, and Flavobacterium. The results of screening with PYBG media obtained 30 isolates of vitamin B12 producing bacteria. Ten efficient vitamin B12-producing isolates were identified as Lysinibacillus fusiformis (isolate G2V1), Bacillus cereus group (isolate G2V25, G2V24, G2V13, G2V9, G2V8), Alcaligenes faecalis (isolate G2V22), Delftia acidovorans (isolate G2V14A) and the genus Delftia (isolate G2V19). The isolate G2V1 (Lysinibacillus fusiformis) was the highest producer of vitamin B12 which was able to produce 33,783 ug/mL of vitamin B12 at 4 days incubation time.

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The vitamin-B12-producing bacteria in the water and sediment of a carp culture pond

Cited by 10 publications

References 11 publications

Novel ecological niche of Cetobacterium somerae, an anaerobic bacterium in the intestinal tracts of freshwater fish

Novel ecological niche of Cetobacterium somerae, an anaerobic bacterium in the intestinal tracts of freshwater fish

The epiphytic microbiota of the globally widespread macroalga Cladophora glomerata (Chlorophyta, Cladophorales)

Exploration of vitamin B12-producing bacteria from Indonesia Eutrophic Lake: A new strategy to improve microalgae biomass production

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