The Virulence of Original and Derived Strains of Semliki Forest Virus for Mice, Guinea-pigs and Rabbits

Bradish, C. J.; Allner, K.; Maber, H. B.

doi:10.1099/0022-1317-12-2-141

Cited by 156 publications

(97 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to the broad host range of the Semliki Forest virus, the obtained viral stock is capable of infecting a wide variety of different cell lines, leading to high levels of recombinant protein expression. Semliki Forest virus is well suited for expression studies, since although wild-type Semliki Forest virus is lethal to mice [30] it has a low pathogenicity in man [311. Moreover, the vector cDNAs used are derived from an attenuated strain [32,331.…”

mentioning

confidence: 99%

High‐level Expression of the Human Neurokinin‐1 Receptor in Mammalian Cell Lines using the Semliki Forest Virus Expression System

Lundström¹,

Mills²,

Buell³

et al. 1994

European Journal of Biochemistry

View full text Add to dashboard Cite

Human neurokinin-1 receptor cDNA was introduced into the pSFVl Semliki Forest virus (SFV) vector and the in vitro transcribed RNA was electroporated into BHK cells with pSFV-Helper RNA. This procedure resulted in the packaging of a high-titer SFV-NK-1 virus stock containing approximately 5 X lo9 infective units/ml. Infection of baby hamster kidney, COS-7, Chinese hamster ovary and human osteosarcoma cells yielded high levels of human neurokinin-1 receptor expression as assessed by [3H]~ub~tance P binding. The maximal receptor expression level obtained was 4X106 receptors/cell and studies of the post-infection time indicated that a high level of receptor expression was observed 10-24 h post-infection. The human neurokinin-1 receptor expressed in infected baby hamster kidney, COS-7 and Chinese hamster ovary cells was able to stimulate Ca2+ mobilization indicating functional coupling to guanine-nucleotide-binding proteins. The application of the Semliki Forest virus expression system will permit the rapid and efficient production of large quantities of receptor protein for both pharmacological and structural studies. [8, 91. Instrumental in our current understanding of the structure and function of this receptor has been its molecular cloning from a number of different species [2, 10, 111 and many reports have appeared on the membrane topology, ligand binding and interaction with G proteins [12-151 of this protein. However, further progress is being hampered by the lack of large quantitities of purified receptor for structural studies. Moreover, in order to elucidate in more detail the structure/function relationships, receptor mutants will need to be investigated. Earlier strategies for testing seven-transmembrane receptor function have included injection of in vitm synthesized mRNA into Xenopus laevis oocytes [16, 171, expression in bacteria [18, 191 and yeast [20, 211, and Correspondence to K. Lundstrom, Department of Molecular BiFax: +41 22 7946965. Abbreviations. BHK, baby hamster kidney; CHO, Chinese hamster ovary; DMEM, Dulbecco's modified Eagle's medium; Fura-2,

show abstract

mentioning

confidence: 99%

High‐level Expression of the Human Neurokinin‐1 Receptor in Mammalian Cell Lines using the Semliki Forest Virus Expression System

Lundström¹,

Mills²,

Buell³

et al. 1994

European Journal of Biochemistry

View full text Add to dashboard Cite

show abstract

“…Absence of protection at Day 7 to SFV after sc injection with the lower dose of 5 mg DDA/kg was demonstrated previously (9). Earlier it was demonstrated that ic immunization of mice with inactivated SFV mixed with DDA (5 mg/kg) resulted in DH 7 days later without demonstrable antibodies (3).…”

Section: Discussionmentioning

confidence: 62%

Enhancement of delayed-type hypersensitivity and induction of interferon by the lipophilic agents DDA and CP-20,961

Kraaijeveld

Snippe

Harmsen

et al. 1982

Cellular Immunology

View full text Add to dashboard Cite

The lipophilic amines dimethyl dioctadecyl ammonium bromide (DDA) and N,iVdioctadecyl-N:N'-bis(2-hydroxyethyl)propanediamine 961) are compared on their capacities to induce interferon, nonspecific protection to viral infection, and enhancement of delayed-type hypersensitivity (DH). DDA, a well-known adjuvant for the induction of DH is a moderate interferon inducer like CP-20,96 1. On the other hand, CP-20.96 1, a known interferon inducer and resembling in structure DDA, is shown to enhance DH to inactivated Semliki Forest virus (WV). Nonspecific protection to challenge with a lethal dose of either SFV or encephalomyocarditis (EMC) virus was induced on injection of both compounds.

show abstract

“…The strains of Semliki Forest virus (SFV) have been described by Bradish et al (1971Bradish et al ( , 1975a. The avirulent A774 strain has been used after three passages in suspensions of primary chick embryo cells (A774.C3).…”

Section: Methodsmentioning

confidence: 99%

“…These were by plaque counting in agar suspensions of primary chick embryo cells (Bradish et al, 1971;Fitzgeorge & Bradish, 1980) prepared at the time of inoculation of the same sample dilutions into mice. The standard diluent was Parker's medium 199 with 10% foetal calf serum, pH 7.4.…”

Section: Methodsmentioning

confidence: 99%

The Effects of Interferon and Double-stranded RNA upon the Virus-Host Interaction: Studies with Togavirus Strains in Mice

Bradish¹,

Titmuss

1981

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

The Virulence of Original and Derived Strains of Semliki Forest Virus for Mice, Guinea-pigs and Rabbits

Cited by 156 publications

References 10 publications

High‐level Expression of the Human Neurokinin‐1 Receptor in Mammalian Cell Lines using the Semliki Forest Virus Expression System

High‐level Expression of the Human Neurokinin‐1 Receptor in Mammalian Cell Lines using the Semliki Forest Virus Expression System

Enhancement of delayed-type hypersensitivity and induction of interferon by the lipophilic agents DDA and CP-20,961

The Effects of Interferon and Double-stranded RNA upon the Virus-Host Interaction: Studies with Togavirus Strains in Mice

Contact Info

Product

Resources

About