The virome of an endangered stingless bee suffering from annual mortality in southern Brazil

Caesar, Lílian; Cibulski, Samuel Paulo; Canal, Cláudio Wageck; Blochtein, Betina; Sattler, Aroni; Haag, Karen Luisa

doi:10.1099/jgv.0.001273

Cited by 28 publications

(19 citation statements)

References 101 publications

(34 reference statements)

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“…The assembled contigs were examined in search for similarities to known sequences with BLASTx software. Sequences with E-values of 10 −3 or less were classified as likely originating from a eukaryotic virus, bacteria, phages, eukaryote or unknown, based on the taxonomic origin of the sequence with the best E-value as described by Caesar et al (2019) .…”

Section: Methodsmentioning

confidence: 99%

A plate of viruses: Viral metagenomics of supermarket chicken, pork and beef from Brazil

et al. 2021

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A viral metagenomics study was conducted in beef, pork, and chicken sold in supermarkets from Southern Brazil. From chicken, six distinct gyroviruses (GyV) were detected, including GyV3 and GyV6, which for the first time were detected in samples from avian species, plus a novel smacovirus species and two highly divergent circular Rep-encoding ssDNA (CRESS-DNA) viruses. From pork, genomes of numerous anelloviruses, porcine parvovirus 5 (PPV5) and 6 (PPV6), two new genomoviruses and two new CRESS-DNA viruses were found. Finally, two new CRESS-DNA genomes were recovered from beef. Although none of these viruses have history of transmission to humans, the findings reported here reveal that such agents are inevitably consumed in diets that include these types of meat.

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Section: Methodsmentioning

confidence: 99%

A plate of viruses: Viral metagenomics of supermarket chicken, pork and beef from Brazil

et al. 2021

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“…Kebiasaannya virus dibawa oleh lebah kelulut semasa proses pendebungaan yang dilakukan ke atas tanaman yang ditanam secara terbuka dan di kawasan rumah hijau (Gisder & Genersch, 2017). Malahan, patogen juga boleh tersebar melalui translokasi sarang (Caesar et al, 2019). Virus ini akan tersebar kepada sarang lebah kelulut, seterusnya menjejaskan kesihatan serta kesejahteraan koloni (Chen & Siede, 2007).…”

Section: Kajian Literaturunclassified

“…Virus ini akan tersebar kepada sarang lebah kelulut, seterusnya menjejaskan kesihatan serta kesejahteraan koloni (Chen & Siede, 2007). Menurut Caesar et al (2019), simptom serangan virus antaranya adalah kematian pupa dan lebah kelulut dewasa, serta pergerakkan kaki yang terbatas seperti merangkak, kaki bergetar dan kelumpuhan. Anggota fizikal turut terjejas antaranya kecacatan pada sayap, bentuk fizikal yang lebih pendek dan mengembung, warna badan yang lebih pudar dan sayap yang menggeletar Keadaan ini menyebabkan perkembangan koloni lebah kelulut menjadi semakin lemah, kadar kematian tinggi, seterusnya menjejaskan proses pendebungaan, terutamanya dalam perkembangan hasil tanaman bagi sektor pertanian.…”

Section: Kajian Literaturunclassified

“…Serangan virus dan kulat ini mampu menggugat pengeluaran hasil madu dan boleh menyebabkan koloni musnah. Gangguan biologikal ini dibuktikan oleh kajian Caesar et al (2019), Rosliza (2010), serta Chen dan Siede (2007) yang mendapati serangan virus dan kulat berupaya menggugat kesihatan dan kesejahteraan populasi koloni, lantas pengeluaran hasil turut terjejas.…”

Section: Gangguan Biologikalunclassified

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Isu dan cabaran usahawan dalam industri penternakan lebah kelulut

et al. 2020

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Wujudnya kesedaran masyarakat terhadap pemakanan sihat menjadikan madu lebah kelulut antara produk yang mendapat permintaan tinggi. Namun begitu, penawarannya adalah rendah di pasaran, berikutan wujudnya pelbagai isu dan cabaran yang menggugat pengeluaran hasil. Tambahan pula, bilangan usahawan yang menceburi bidang ini masih di tahap minimum. Oleh itu, kajian ini dilakukan untuk menilai isu dan cabaran yang dihadapi oleh usahawan penternakan lebah kelulut di Kuala Lipis, Pahang. Reka bentuk gabungan kaedah penyelidikan kuantitatif dan kualitatif dilaksanakan ke atas 32 usahawan ternakan lebah kelulut. Hasil kajian mendapati gangguan fizikal melibatkan serangan serangga dan penyakit, monyet, burung pemakan serangga serta kecurian log kelulut oleh manusia merupakan isu serta cabaran utama yang dihadapi oleh usahawan. Gangguan aktiviti ekonomi manusia seperti penebangan hutan, penggunaan racun di kawasan pertanian dan kebakaran hutan, serta faktor musiman dan tahap pengetahuan usahawan yang rendah dalam proses pengeluaran turut menjadi isu dan cabaran dalam aktiviti penternakan lebah kelulut. Lantaran itu, penglibatan kukuh usahawan dalam industri penternakan lebah kelulut perlu mendapat sokongan padu daripada agensi pertanian dan agensi sokongan lain dalam memberi khidmat nasihat dan pengembangan. Selain itu, penyelidikan dan pembangunan terhadap potensi lebah kelulut perlu dipergiat bagi mengurangkan risiko dan cabaran yang dihadapi oleh usahawan. Pendedahan kepada penggunaan teknologi 4.0 dalam penternakan lebah kelulut perlu diperluas kepada usahawan bagi melahirkan usahawan yang berdaya saing selari dengan perkembangan Revolusi Pertanian 4.0.

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“…Nowadays, the application of metagenomics accelerates the studies of viral identification, persistence, spread, and interaction with both their insect hosts and other microbes. For example, using metagenomic analysis, seven novel positive-strand RNA viruses, belonging to the families Dicistroviridae, Parvoviridae, and Circoviridae, are identified in an important stingless bee Melipona quadrifasciata which have the largest copy numbers in unhealthy bees and may be pathogenic (7). Eight novel RNA viruses, including four positive-strand RNA viruses, two negative-strand RNA viruses, and two double-stranded RNA, are discovered in the high-throughput sequencing data of an important invasive agricultural insect pest, the oriental fruit fly Bactrocera dorsalis (8).…”

mentioning

confidence: 99%

Diverse RNA Viruses Discovered in Three Parasitoid Wasps of the Rice Weevil Sitophilus oryzae

Wang

Yuan

Xiao

et al. 2021

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In this study, many virus-like fragments were obtained from transcriptomes of three wasp species, including Anisopteromalus calandrae (8), Lariophagus distinguendus (3), and Theocolax elegans (18), which can parasitize and control rice weevil Sitophilus oryzae, a serious insect pest of farm-stored grains. By further bioinformatic analysis and sequencing, we identified six novel RNA viruses with complete genomes and named them WWPSRV-1, WWPSRV-2, AcPSRV-1, AcNSRV-1, AcNSRV-2, and LdNSRV-1. PCR-based detection revealed that WWPSRV-1 and WWPSRV-2 had the possibility of interspecies virus transmission, especially WWPSRV-2, which was also present in the rice weevil adults. Phylogenetically, three out of these six viruses appeared to be members of order Picornavirales: WWPSRV-1 belonged to unassigned virus families of this order, whereas WWPSRV-2 and AcPSRV-1 belonged to families Iflaviridae and Dicistroviridae, respectively. The conserved picornavirus-typical domains helicase, protease, and RNA-dependent RNA polymerase could be found in the nonstructural protein encoded by the three viruses, whose genomes consisted of the different numbers of open reading frames (ORFs). The other three RNA viruses could be classified to order Mononegavirales: AcNSRV-1 and AcNSRV-2 belonged to family Lispiviridae, whereas LdNSRV-1 belonged to a big family Rhabdoviridae. The genomes of the three viruses contained at least five ORFs, encoding deduced proteins in the following order: 3′-N-P-M-G-L-5′. All the ORFs were separated by conserved intergenic sequences which likely regulated the transcription termination and initiation. Our findings enhance the understanding of RNA viruses in weevil wasps and set the foundation for the future study of the association among weevils, weevil wasps, and RNA viruses. IMPORTANCE The enormous diversity of RNA viruses in insects is continuously validated. Parasitoid wasps, as biocontrol insects which are widely used against insect pests in agroecosystems, may also carry many “good” RNA viruses. Some RNA viruses in parasitoid wasps have been reported to affect the host wasps or the wasps’ host. Here, six novel RNA viruses with complete genomes were identified in three parasitoid wasps of the rice weevil. One of these viruses was also detected in the rice weevil adults. Phylogenetically, WWPSRV-1 was the first unambiguous detection of Nora-like virus in insect parasitoids. WWPSRV-2 and AcPSRV-1 belong to families Iflaviridae and Dicistroviridae, some viruses of which can result in lethal infections in silkworms and honeybees. The other three RNA viruses belong to order Mononegavirales, which comprises many well-known insect-associated viruses.

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The virome of an endangered stingless bee suffering from annual mortality in southern Brazil

Cited by 28 publications

References 101 publications

A plate of viruses: Viral metagenomics of supermarket chicken, pork and beef from Brazil

A plate of viruses: Viral metagenomics of supermarket chicken, pork and beef from Brazil

Isu dan cabaran usahawan dalam industri penternakan lebah kelulut

Diverse RNA Viruses Discovered in Three Parasitoid Wasps of the Rice Weevil Sitophilus oryzae

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