The virion RNA species of the Kirsten murine sarcoma-leukemia virus complex released from a clonally related series of mouse cells

Clewley, J. P.; Avery, R. J.

doi:10.1007/bf01314448

Cited by 10 publications

(9 citation statements)

References 19 publications

(3 reference statements)

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“…By analogy with retroviruses, infection is probably accomplished by reverse transcription of VL30 RNA to produce "proviral" VL30 DNA which then integrates into host cell DNA. VL30 RNA thus resembles a defective retrovirus genome although nucleic acid hybridization and oligonucleotide mapping studies have not revealed any significant homology with known murine leukemia virus genomes [2][3][4]6].…”

Section: Introductionmentioning

confidence: 99%

A novel approach to cloning transcriptionally active retrovirus-like genetic elements from mouse cells

Carter¹,

Norton²,

Avery

1983

Nucl Acids Res

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A family of dispersed, moderately repeated mouse genetic elements is expressed as retrovirus-like 30S RNA species (VL30 RNA) which can be transmitted to other cells when packaged as a pseudovirion complex by murine leukemia viruses (MuLV). Using the endogenous reverse transcriptase reaction of VL30 RNA-containing MuLV particles, full-length VL30 DNA was synthesized and cloned in pAT153. Analysis of a number of clones identified long terminal repeat structures (LTRs) characteristic of retrovirus proviruses and transposable genetic elements. Whilst the unique region of all clones was identical, the LTRs displayed some heterogeneity. Comparison of the unique region of cloned VL30 DNA with mouse genomic VL30 sequences showed the retrovirus-derived clones to be encoded by only a few members of the divergent VL30 gene family. These findings thus demonstrate a method for cloning a defined sub-class of retrovirus-like cellular genes which are both transcriptionally active and transmissible by a retrovirus.

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Section: Introductionmentioning

confidence: 99%

A novel approach to cloning transcriptionally active retrovirus-like genetic elements from mouse cells

Carter¹,

Norton²,

Avery

1983

Nucl Acids Res

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“…Analytical gel electrophoresis of viral RNA species. RNA species were resolved by electrophoresis in 1% (wt/vol) agarose gels in 50% (vol/vol) formamide by using a horizontal, submerged gel system as previously described (3).…”

Section: Methodsmentioning

confidence: 99%

Two Unique RNA Species of the Nodavirus Black Beetle Virus

Clewley

Crump

Avery

et al. 1982

J Virol

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Two-dimensional fingerprinting of RNase T1-derived oligonucleotides of the two individual RNA segments of the Nodavirus black beetle virus indicates that each RNA species possesses a distinct nucleotide sequence. Species 1 RNA has a genome complexity of approximately 3,000 nucleotides, and species 2 RNA is composed of approximately 1,500 nucleotides. Submolar amounts of oligonucleotides apparently derived from a third virus-specific RNA were also detected in black beetle virus RNA preparations.

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“…A typical element is 5 to 6 kb in length and contains long terminal repeats (LTRs) of about 500 nucleotides (nt) with an organization resembling that of retroviral LTRs, namely U3-R-U5 (16,27,37,47). The major 30S VL30 RNA transcript is expressed at high levels and in a variety of cell types (10,16,22,34). VL30m elements do not encode virus-like proteins, have little sequence homology to Moloney murine leukemia virus (MoMLV) and have not been implicated in retroviral carcinogenesis (16,27).…”

mentioning

confidence: 99%

Identification of an Internal Ribosome Entry Segment in the 5′ Region of the Mouse VL30 Retrotransposon and Its Use in the Development of Retroviral Vectors

López-Lastra

Ulrici

Gabus

et al. 1999

J Virol

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Mouse virus-like 30S RNAs (VL30m) constitute a family of retrotransposons, present at 100 to 200 copies, dispersed in the mouse genome. They display little sequence homology to Moloney murine leukemia virus (MoMLV), do not encode virus-like proteins, and have not been implicated in retroviral carcinogenesis. However, VL30 RNAs are efficiently packaged into MLV particles that are propagated in cell culture. In this study, we addressed whether the 5′ region of VL30m could replace the 5′ leader of MoMLV functionally in a recombinant vector construct. Our data confirm that the putative packaging sequence of VL30 is located within the 5′ region (nucleotides 362 to 1149 with respect to the cap structure) and that it can replace the packaging sequence of MoMLV. We also show that VL30m contains an internal ribosome entry segment (IRES) in the 5′ region, as do MoMLV, Friend murine leukemia virus, Harvey murine sarcoma virus, and avian reticuloendotheliosis virus type A. Our data show that both the packaging and IRES functions of the 5′ region of VL30m RNA can be efficiently used to develop retrotransposon-based vectors.

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The virion RNA species of the Kirsten murine sarcoma-leukemia virus complex released from a clonally related series of mouse cells

Cited by 10 publications

References 19 publications

A novel approach to cloning transcriptionally active retrovirus-like genetic elements from mouse cells

A novel approach to cloning transcriptionally active retrovirus-like genetic elements from mouse cells

Two Unique RNA Species of the Nodavirus Black Beetle Virus

Identification of an Internal Ribosome Entry Segment in the 5′ Region of the Mouse VL30 Retrotransposon and Its Use in the Development of Retroviral Vectors

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