The Vibrio cholerae O1 chromosomal integron The GenBank accession numbers for the sequences determined in this work are AF025662, AF055586, X64097, AF179593 and AF179596.

Clark, Christopher A.; Purins, Leanne; Kaewrakon, Pranom; Focareta, Tony; Manning, Paul A.

doi:10.1099/00221287-146-10-2605

Cited by 68 publications

(57 citation statements)

References 26 publications

(33 reference statements)

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“…The MGC size class profiles for O1 classical, O1 El Tor and O139 were relatively conserved (except for O139 Arg3 and O1 El Tor C31), which is consistent with earlier studies (Clark et al, 2000) and confirms that pandemic V. cholerae isolates have evolved from a common progenitor. A dendrogram based on the presence/absence of MGC size classes was able to resolve phylogenetic relationships between pandemic V. cholerae strains that previous singleor multi-locus phylogenetic studies have been unable to do.…”

Section: Discussionsupporting

confidence: 79%

“…As noted above, the VCR-PCR method (Castañeda et al, 2005) does not have the high resolution produced here. A method that utilizes RFLP and a 59-be probe (Clark et al, 2000) is able to identify general conserved fragments between strains; however, specific MGCs differing between strains cannot be identified. When a microarray based on N16961 is used (Dziejman et al, 2005), only comparisons with N16961 can be made, and only MGCs present in N16961 and absent in the tested strain can be identified.…”

Section: Discussionmentioning

confidence: 99%

“…Other studies (Clark et al, 2000) have shown that the integron MGC arrays of C31 and Arg3 are different from those of the other pandemic strains. A deletion in O1 El Tor C31 compared to other O1 V. cholerae in a region now identified as the MGC array has been reported in earlier studies (Barker & Clark, 1994;van Dongen & DeGraaf, 1986).…”

Section: Validation Of the Gene Cassette Size Class Profiling Techniquementioning

confidence: 99%

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Use of chromosomal integron arrays as a phylogenetic typing system for Vibrio cholerae pandemic strains

et al. 2007

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Approximately 200 serogroups of Vibrio cholerae exist, with only two, O1 and O139, responsible for epidemic and pandemic cholera. Strains from these serogroups have evolved from a common progenitor, with lateral gene transfer largely driving their emergence. These strains are so closely related that separation using single-or multi-locus phylogeny has proven difficult. V. cholerae strains contain a genetic system called the integron that is located in the chromosome and that can integrate and excise DNA elements called mobile gene cassettes (MGCs) by site-specific recombination. Large arrays of MGCs are found in V. cholerae strains. For instance, the O1 El Tor strain N16961 contains 179 MGCs. Since integron arrays are dynamic through recombination and excision of MGCs, it was hypothesized that the MGC composition in a given V. cholerae pandemic strain would be useful as a phylogenetic typing system. To address this, a PCR-based method was used to rapidly characterize the MGC composition of V. cholerae arrays. The results showed that the MGC composition of pandemic V. cholerae cassette arrays is relatively conserved, providing further evidence that these strains have evolved from a common progenitor. Comparison of MGC composition between the V. cholerae pandemic strains was also able to resolve the evolution of O139 from a subgroup of O1 El Tor. This level of differentiation of closely related V. cholerae isolates was more sensitive than conventional single-gene phylogeny or multi-locus sequence analysis. Using this method, novel MGCs from an O1 classical strain and an Argentinian O139 isolate were also identified, and a major deletion in the MGC array in all pandemic O139 strains and a subset of O1 El Tor strains was identified. Analysis of sequenced V. cholerae integron arrays showed that their evolution can proceed by rearrangements and deletions/insertions of large portions of MGCs in addition to the insertion or excision of single MGCs.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Validation Of the Gene Cassette Size Class Profiling Techniquementioning

confidence: 99%

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Use of chromosomal integron arrays as a phylogenetic typing system for Vibrio cholerae pandemic strains

et al. 2007

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“…Again, none of the known cassettes in these Pseudomonas species are found in more than one array. In the genus Vibrio, integrons can contain hundreds of unique gene cassettes (17,42). Hence, it seems likely that the arrays in Xanthomonas pathovars will also consist of multiple cassettes, and that many of these cassettes will be found in only one pathovar.…”

Section: Discussionmentioning

confidence: 99%

Integrons inXanthomonas: A source of species genome diversity

Gillings

Holley

Stokes

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

130

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Integrons are best known for assembling antibiotic resistance genes in clinical bacteria. They capture genes by using integrasemediated site-specific recombination of mobile gene cassettes. Integrons also occur in the chromosomes of many bacteria, notably ␤-and ␥-Proteobacteria. In a survey of Xanthomonas, integrons were found in all 32 strains representing 12 pathovars of two species. Their chromosomal location was downstream from the acid dehydratase gene, ilvD, suggesting that an integron was present at this site in the ancestral xanthomonad. There was considerable sequence and structural diversity among the extant integrons. The majority of integrase genes were predicted to be inactivated by frameshifts, stop codons, or large deletions, suggesting that the associated gene cassettes can no longer be mobilized. In support, groups of strains with the same deletions or stop codons͞frameshifts in their integrase gene usually contained identical arrays of gene cassettes. In general, strains within individual pathovars had identical cassettes, and these exhibited no similarity to cassettes detected in other pathovars. The variety and characteristics of contemporary gene cassettes suggests that the ancestral integron had access to a diverse pool of these mobile elements, and that their genes originated outside the Xanthomonas genome. Subsequent inactivation of the integrase gene in particular lineages has largely fixed the gene cassette arrays in particular pathovars during their differentiation and specialization into ecological niches. The acquisition of diverse gene cassettes by different lineages within Xanthomonas has contributed to the species-genome diversity of the genus. The role of gene cassettes in survival on plant surfaces is currently unknown. genome evolution ͉ lateral gene transfer ͉ mobile DNA ͉ pathovar

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“…Class 4 integron has been identified and characterized among the Vibrionaceae, Shewanella, Xanthomonas, Pseudomonas, as well as other proteobacteria (Clark et al, 2000;Heidelberg, 2000;Nield et al, 2001;. The remaining classes of integrons may also contain antibiotic resistance gene cassettes, but their worldwide prevalence remains low Nield et al, 2001).…”

Section: Class 4 Integron: Super Integronmentioning

confidence: 99%

Role of integrons in antimicrobial resistance: A review

Gao¹,

Li²,

Liu³

et al. 2013

Afr. J. Microbiol. Res.

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Antibiotic resistance in bacteria still remains one of the leading concerns in global public health. Horizontal transfer of resistance genes is considered to be a major cause to facilitate the rapid spread of antibiotic resistance in microbes. In recent years, the role of integrons as mobile genetic elements playing a central role in horizontal transfer of antibiotic resistance have been well studied and documented. Nevertheless, up to date, most of the available investigations and studies on integron had focused on class 1 integrons of Gram-negative microorganisms. In the last decade, class 4 integrons (also known as super integrons) has been considered a major concern in antimicrobial resistance, as well as, a significant factor in bacterial genome evolution; however, most reports had been limited to Vibrio. Thus, this review is aimed at summarizing the occurrence and prevalence of integrons in bacterial isolates, with the focus on class 1 integron within Gram-positive bacteria, class 2 and 3 integrons.

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The Vibrio cholerae O1 chromosomal integron The GenBank accession numbers for the sequences determined in this work are AF025662, AF055586, X64097, AF179593 and AF179596.

Cited by 68 publications

References 26 publications

Use of chromosomal integron arrays as a phylogenetic typing system for Vibrio cholerae pandemic strains

Use of chromosomal integron arrays as a phylogenetic typing system for Vibrio cholerae pandemic strains

Integrons inXanthomonas: A source of species genome diversity

Role of integrons in antimicrobial resistance: A review

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