The viability of late morulae and blastocysts produced by nuclear transplantation in cattle

Willadsen, S. M.; Janzen, R.E.; McAlister, R.J.; Shea, Brian F.; Hamilton, Greg; McDermand, D.P.

doi:10.1016/0093-691x(91)90155-7

Cited by 155 publications

(69 citation statements)

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“…This finding is supported by studies in cattle (Behboodi et al, 1995;Farin and Farin, 1995 (Willadsen et al, 1991;Wilson et al, 1995 (Behboodi et al, 1995). Thompson et al (1995) (Wilmut and Sales, 1981) or to the oviducts of progesterone treated ewes (Kleemann et al, 1994) causes enhanced fetal growth.…”

Section: Discussionsupporting

confidence: 75%

“…Thompson et al (1995) (Wilmut and Sales, 1981) or to the oviducts of progesterone treated ewes (Kleemann et al, 1994) causes enhanced fetal growth. In cattle, in vitro procedures also affect fetal growth (Behboodi et al, 1995;Farin and Farin, 1995), but not duration of gestation (Behboodi et al, 1995), while cloning by nucleus transfer affects both (Willadsen et al, 1991;Wilson et al, 1995). The numbers of lambs and variation in birth weights in this study do not allow further conclusions regarding the possible relations between the type of manipulation, birth weight and duration of gestation.…”

Section: Discussioncontrasting

confidence: 42%

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Embryo viability, duration of gestation and birth weight in sheep after transfer of in vitro matured and in vitro fertilized zygotes cultured in vitro or in vivo

Holm¹,

Walker²,

Seamark³

1996

Reproduction

130

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The influence of various in vitro procedures on embryo survival and the production of normal offspring was investigated in sheep. Zygotes with both in vivo cultured IVM\p=n-\IVF zygotes and control zygotes 25\p=n-\35% versus 51\p=n-\60%, respectively, P < 0.05). Day 6.5 IVM\p=n-\IVFmorulae had a lower survival rate than did control morulae regardless of culture treatment (P < 0.05), while survival rates of day 6.5 IVM\p=n-\IVF blastocysts cultured in vivo did not differ from those of control blastocysts (P > 0.1). Both the gestation period and birth weight of IVM\p=n-\IVFlambs were increased when compared with controls, the former significantly in all groups (154.0\p=n-\154.9days versus 150.6 days; P < 0.01), while the latter increase was on the borderline of significance (4\ m=. \ 5\ p=n-\ 4\ m=. \ 8 kg versus 4.0 kg; 0.01 \m=le\P \m=le\0.1, respectively) and dependent on the prolongation of the gestation period. It is concluded that in vitro maturation and fertilization compromise subsequent embryonic and fetal development in sheep irrespective of the subsequent in vivo or in vitro culture treatment. Subjecting IVM\p=n-\IVF zygotes to in vivo culture for 6.5 days minimizes only some of these effects, thus leading to the aberrant production of some offspring.

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Section: Discussionsupporting

confidence: 75%

Section: Discussioncontrasting

confidence: 42%

Embryo viability, duration of gestation and birth weight in sheep after transfer of in vitro matured and in vitro fertilized zygotes cultured in vitro or in vivo

Holm¹,

Walker²,

Seamark³

1996

Reproduction

130

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“…The high number of excluded or degenerating cells found in some embryos may partially explain early embryonic mortality that follows transfer. However, these observations do not give a clear explanation for (Bondioli et al, 1990;Willadsen et al, 1991;Chesne et al, 1993 (Heyman et al, 1994). This may indicate that the quality of the transferred blastocysts is suboptimal.…”

mentioning

confidence: 44%

Cellular evaluation of bovine nuclear transfer embryos developed in vitro

Heyman¹,

Degrolard²,

Adenot³

et al. 1995

Reprod. Nutr. Dev.

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“…Indeed, electrical activation of the in vitro matured bovine oocytes was found to be oocyteage-dependent and poor activation results were obtained with oocytes matured in vitro less than 30 h (Ware et al, 1989;Yang et al, 1991 Thus, at one time, most nuclear transfer programs involved using aged oocytes as recipients (Willadsen et al, 1991;Westhusin et al, 1992;Yang et al, 1993a). More recently, improvements on activation procedures have resulted in suc-cessful activation and led to the use of young oocytes for nuclear transfer (First et al, 1992;Barnes et al, 1993;Yang et al, 1993b;Aoyagi ef al, 1994;Heyman et al, 1994;Stice et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Beneficial effect of oocyte activation prior to and during nuclear transfer in cattle using in vitro matured oocytes 24 h of age

Du¹,

Jiang²,

Yang³

1995

Reprod. Nutr. Dev.

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Summary ― This research was designed to study in vitro development of bovine nuclear transferred embryos using enucleated young in vitro matured oocytes 24 h of age as recipient cytoplasts activated prior to nuclear transfer. The oocytes were enucleated and then activated with electric pulse at 24 h of age followed by incubation with cycloheximide for 6-7 h before nuclear transfer and membrane fusion with a blastomere of the [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32]

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The viability of late morulae and blastocysts produced by nuclear transplantation in cattle

Cited by 155 publications

References 1 publication

Embryo viability, duration of gestation and birth weight in sheep after transfer of in vitro matured and in vitro fertilized zygotes cultured in vitro or in vivo

Embryo viability, duration of gestation and birth weight in sheep after transfer of in vitro matured and in vitro fertilized zygotes cultured in vitro or in vivo

Cellular evaluation of bovine nuclear transfer embryos developed in vitro

Beneficial effect of oocyte activation prior to and during nuclear transfer in cattle using in vitro matured oocytes 24 h of age

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