The viability of human dental pulp cells and apical papilla cells after treatment with conventional calcium hydroxide and nanoparticulate calcium hydroxide at various concentrations

Pugdee, Kamolparn; Klaisiri, Awiruth; Phumpatrakom, Panupat

doi:10.1016/j.sdentj.2023.08.001

Cited by 2 publications

(2 citation statements)

References 33 publications

(39 reference statements)

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“…25 This agrees previous studies reporting increased cell survival of MSCs exposed to CH 26 , or L929 murine fibroblasts exposed to CH and NCH. 27 However, our results partially agree Pudgee et al 28 who reported significantly less survival of primary human dental pulp cells cultured for 1, 3, 5 , and 7 days with NCH in comparison to conventional CH.…”

Section: Asdj Ain Shams Dental Journalsupporting

confidence: 86%

Effect of different calcium hydroxide formulations used during regenerative endodontic procedures on the viability, mineralization, and dentino/cemento/osteogenic differentiation potential of human periodontal ligament stem cells. An in-vitro study.

Saber

2024

Ain Shams Dental Journal

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To evaluate the effect of CH and NCH on the viability, attachment, osteogenic, cementogenic and dentinogenic differentiation potential of hPDLSCs in-vitro. Materials and Methods: Calcium Hydroxide (CH) and nano calcium hydroxide (NCH) powders were initially characterized using transmission electron microscope (TEM), X-ray Diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and a zeta sizer instrument. Standard dentin discs were prepared, irrigated according to the Regenerative Endodontic Procedures (REP) protocol, then treated with 1 mg/ml of CH or NCH pastes, and incubated for 1 week. Untreated discs served as control. Then, they were washed off, and all discs were treated with 17% EDTA before seeding of human periodontal ligament stem cells (hPDLSCs) . Cell viability was assessed using MTT assay. Mineralized nodules and alkaline phosphatase (ALP) activity were determined to assess cell differentiation. Cell dentino/cementogenic and osteogenic potential were assessed through monitoring gene expression levels Dentin sialophosphoprotein (DSSP), Cementum protein 1 (CEMP1), Runt-related transcription factor 2 (RUNX2), Osteoprotegrin (OG), and Osteocalcin (OC) using RT-qPCR. Results: TEM examination of NCH powder particles revealed a regular pattern and hexagonal shape with side dimension up to 60 ±10 nm. XRD analysis of NCH powder demonstrated its highly crystalline nature. FTIR spectroscopy confirmed that CH and NCH consisted mainly of pure calcium hydroxide. The zeta sizer instrument showed that NCH is positively charged with an average zeta potential of +27.8 mV. hPDLSCs seeded on NCH-treated discs showed significantly higher cell survival , more mineralization, higher alkaline phosphatase activity and increased expression of DSPP, CEMP1, RUNX2, OPG and OC markers. Conclusion: NCH can be an alternative ICM during REP.

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Section: Asdj Ain Shams Dental Journalsupporting

confidence: 86%

Effect of different calcium hydroxide formulations used during regenerative endodontic procedures on the viability, mineralization, and dentino/cemento/osteogenic differentiation potential of human periodontal ligament stem cells. An in-vitro study.

Saber

2024

Ain Shams Dental Journal

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“…The choice of pulp capping material in any of the three techniques mentioned above relates to its ability induce the formation of mineralized tissue and maintain tooth vitality (Fousolas et al, 2023). Therefore, the material selected for this procedure significantly influences the treatment prognosis ( Pugdee, Klaisiri, Phumpatrakom, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Exploring vital pulp Therapies: A bibliometric analysis of the most cited articles

Sousa,

Gonçalves,

Figueiredo

et al. 2024

The Saudi Dental Journal

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The viability of human dental pulp cells and apical papilla cells after treatment with conventional calcium hydroxide and nanoparticulate calcium hydroxide at various concentrations

Cited by 2 publications

References 33 publications

Effect of different calcium hydroxide formulations used during regenerative endodontic procedures on the viability, mineralization, and dentino/cemento/osteogenic differentiation potential of human periodontal ligament stem cells. An in-vitro study.

Effect of different calcium hydroxide formulations used during regenerative endodontic procedures on the viability, mineralization, and dentino/cemento/osteogenic differentiation potential of human periodontal ligament stem cells. An in-vitro study.

Exploring vital pulp Therapies: A bibliometric analysis of the most cited articles

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