The Versatile Roles of Nerve Growth Factor in Neuronal Attraction, Odontoblast Differentiation, and Mineral Deposition in Human Teeth

Mitsiadis, Thimios A.; Pagella, Pierfrancesco

doi:10.1007/978-3-030-74046-7_6

Cited by 3 publications

(2 citation statements)

References 42 publications

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“…NGF is also upregulated in odontoblast-like cells located close to DPA neurons for the repair of teeth and pulp cells in damaged teeth. 62 This condition gives NGF release from odontoblasts a much greater potential to affect adjacent nerve cells. In addition to their mechanosensory functions, DPA neurons exhibit rapid responses to noxious stimuli, such as heat or cold.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional profiling of dental sensory and proprioceptive trigeminal neurons using single-cell RNA sequencing

Lee,

Kim,

Rossi

et al. 2023

Int J Oral Sci

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Dental primary afferent (DPA) neurons and proprioceptive mesencephalic trigeminal nucleus (MTN) neurons, located in the trigeminal ganglion and the brainstem, respectively, are essential for controlling masticatory functions. Despite extensive transcriptomic studies on various somatosensory neurons, there is still a lack of knowledge about the molecular identities of these populations due to technical challenges in their circuit-validated isolation. Here, we employed high-depth single-cell RNA sequencing (scRNA-seq) in combination with retrograde tracing in mice to identify intrinsic transcriptional features of DPA and MTN neurons. Our transcriptome analysis revealed five major types of DPA neurons with cell type-specific gene enrichment, some of which exhibit unique mechano-nociceptive properties capable of transmitting nociception in response to innocuous mechanical stimuli in the teeth. Furthermore, we discovered cellular heterogeneity within MTN neurons that potentially contribute to their responsiveness to mechanical stretch in the masseter muscle spindles. Additionally, DPA and MTN neurons represented sensory compartments with distinct molecular profiles characterized by various ion channels, receptors, neuropeptides, and mechanoreceptors. Together, our study provides new biological insights regarding the highly specialized mechanosensory functions of DPA and MTN neurons in pain and proprioception.

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Section: Discussionmentioning

confidence: 99%

Transcriptional profiling of dental sensory and proprioceptive trigeminal neurons using single-cell RNA sequencing

Lee,

Kim,

Rossi

et al. 2023

Int J Oral Sci

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“…In the study by Pagella and colleagues, the compartmentalization method was utilized to investigate the multifunctional role of NGFs in human dental neuron attraction, odontoblast differentiation, and mineral deposition. [ 220 ] Human pulp cells and the trigeminal ganglion were cultured in separate compartments, and the nerve fibers were allowed to innervate the pulp cells through the microchannels of the microfluidic device. The results revealed the association between NGF and odontoblast differentiation, dentine formation, and neuronal network organization within the dental pulp across various stages including development, pathology, and regeneration.…”

Section: Innervated Msk Tissues For In Vitro Disease Modelingmentioning

confidence: 99%

Engineering Innervated Musculoskeletal Tissues for Regenerative Orthopedics and Disease Modeling

Zhou,

Liu,

Xiong

et al. 2024

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Musculoskeletal (MSK) disorders significantly burden patients and society, resulting in high healthcare costs and productivity loss. These disorders are the leading cause of physical disability, and their prevalence is expected to increase as sedentary lifestyles become common and the global population of the elderly increases. Proper innervation is critical to maintaining MSK function, and nerve damage or dysfunction underlies various MSK disorders, underscoring the potential of restoring nerve function in MSK disorder treatment. However, most MSK tissue engineering strategies have overlooked the significance of innervation. This review first expounds upon innervation in the MSK system and its importance in maintaining MSK homeostasis and functions. This will be followed by strategies for engineering MSK tissues that induce post‐implantation in situ innervation or are pre‐innervated. Subsequently, research progress in modeling MSK disorders using innervated MSK organoids and organs‐on‐chips (OoCs) is analyzed. Finally, the future development of engineering innervated MSK tissues to treat MSK disorders and recapitulate disease mechanisms is discussed. This review provides valuable insights into the underlying principles, engineering methods, and applications of innervated MSK tissues, paving the way for the development of targeted, efficacious therapies for various MSK conditions.

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Roles of centrioles in neural attraction of dental pulp stem cells

Pei,

Chen,

Chen

2023

Journal of the Formosan Medical Association

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The Versatile Roles of Nerve Growth Factor in Neuronal Attraction, Odontoblast Differentiation, and Mineral Deposition in Human Teeth

Cited by 3 publications

References 42 publications

Transcriptional profiling of dental sensory and proprioceptive trigeminal neurons using single-cell RNA sequencing

Transcriptional profiling of dental sensory and proprioceptive trigeminal neurons using single-cell RNA sequencing

Engineering Innervated Musculoskeletal Tissues for Regenerative Orthopedics and Disease Modeling

Roles of centrioles in neural attraction of dental pulp stem cells

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