The versatile roles and clinical implications of exosomal mRNAs and microRNAs in cancer

Tang, Shuli; Yu, Su Jeong; Cheng, Jianan; Zhang, Yanqiao; Huang, Xiaolun

doi:10.1177/1724600820920293

Cited by 6 publications

(3 citation statements)

References 175 publications

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“…To sum up, these findings provide new understandings in the mechanism involved in asthma progression. The clinical application potential of exosomes and the exosomal miRNAs have provided new hopes in the management of human diseases [40][41][42]. We hope the findings of this study may provide the theoretical basis for the potential clinical usage of M2Φ-Exos in the management of asthma.…”

Section: Discussionmentioning

confidence: 74%

MicroRNA-370 carried by M2 macrophage-derived exosomes alleviates asthma progression through inhibiting the FGF1/MAPK/STAT1 axis

Li¹,

Deng²,

Zhou³

et al. 2021

Int. J. Biol. Sci.

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Section: Discussionmentioning

confidence: 74%

MicroRNA-370 carried by M2 macrophage-derived exosomes alleviates asthma progression through inhibiting the FGF1/MAPK/STAT1 axis

Li¹,

Deng²,

Zhou³

et al. 2021

Int. J. Biol. Sci.

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“…Exosome contents have a significant influence on how they affect recipient cells. It has been proposed that the miRNA contents of circulating exosomes have similarities to those in cancer cells from which they originated, highlighting the possibility of using miRNA in cancer diagnostics [56][57][58]. The ability to detect exosomal miRNA in bodily fluids demonstrates the possible benefits of exosomal miRNA as disease biomarkers [59].…”

Section: The Role Of Exosomesmentioning

confidence: 99%

Exosomes: a new perspective in EGFR-mutated lung cancer

Jouida

McCarthy

Fabre

et al. 2021

Cancer Metastasis Rev

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Exosomes are major contributors in cell to cell communication due to their ability to transfer biological material such as protein, RNA, DNA, and miRNA. Additionally, they play a role in tumor initiation, promotion, and progression, and recently, they have emerged as a potential source of information on tumor detection and may be useful as diagnostic, prognostic, and predictive tools. This review focuses on exosomes from lung cancer with a focus on EGFR mutations. Here, we outline the role of exosomes and their functional effect in carcinogenesis, tumor progression, and metastasis. Finally, we discuss the possibility of exosomes as novel biomarkers in early detection, diagnosis, assessment of prognosis, and prediction of therapeutic response in EGFR-mutated lung cancer.

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“…Exosomes are small extracellular vesicles ranging from 30 to 100 nm found in eukaryotic fluids that facilitate many cellular functions, including modulation of tumor phenotypes within and between tumor cells [ 9 , 10 ]. Many microRNAs are produced and then exported extracellularly in exosomes [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

miR-365 (microRNA): Potential Biomarker in Oral Squamous Cell Carcinoma Exosomes and Extracellular Vesicles

Coon

Kingsley

Howard

2020

IJMS

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Introduction: miR-365 is a non-coding microRNA that regulates transcription and has been demonstrated to promote oncogenesis and metastasis in some cancers, while suppressing these effects in others. Many microRNAs are produced and then exported extracellularly in exosomes, which are small extracellular vesicles ranging from 30 to 100 nm that are found in eukaryotic fluids and facilitate many cellular functions. Exosomes and extracellular vesicles are produced by many cell types, including oral cancer cells—although no study to date has evaluated miR-365 and oral cancer exosomes or extracellular vesicles. Based on this information, our research question was to evaluate whether oral cancers produce exosomes or extracellular vesicles containing miR-365. Materials and Methods: Two commercially available oral cancer cell lines (SCC25 and CAL27) and a normal oral keratinocyte (OKF4) were grown in serum-free media, supplemented with exosome-depleted fetal bovine serum. Extracellular vesicles and exosomes were then isolated using the Invitrogen total exosome RNA and protein isolation kit for processing using the hsa-miR-365a-5p microRNA qPCR assay kit. Results: RNA was successfully isolated from the exosome-depleted supernatant from each cell line—SCC9, SCC15, SCC25, and CAL27 (oral squamous cell carcinomas) and OKF4 (oral epithelial cell line). Relative concentrations of RNA were similar among each cell line, which were not significantly different, p = 0.233. RNA quality was established by A260:A280 absorbance using a NanoDrop, revealing purity ranging 1.73–1.86. Expression of miR-16 was used to confirm the presence of microRNA from the extracted exosomes and extracellular vesicles. The presence of miR-365 was then confirmed and normalized to miR-16 expression, which demonstrated an increased level of miR-365 in both CAL27 and SCC25. In addition, the normalized relative quantity (RQ) for miR-365 exhibited greater variation among SCC25 (1.382–4.363) than CAL27 cells (1.248–1.536). Conclusions: These results confirm that miR-365 is not only expressed in oral cancer cell lines, but also is subsequently exported into exosomes and extracellular vesicles derived from these cultures. These data may help to contextualize the potential for this microRNA to contribute to the phenotypes and behaviors of oral cancers that express this microRNA. Future research will begin to investigate these potential mechanisms and pathways and to determine if miR-365 may be useful as an oral cancer biomarker for salivary or liquid biopsies.

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The versatile roles and clinical implications of exosomal mRNAs and microRNAs in cancer

Cited by 6 publications

References 175 publications

MicroRNA-370 carried by M2 macrophage-derived exosomes alleviates asthma progression through inhibiting the FGF1/MAPK/STAT1 axis

MicroRNA-370 carried by M2 macrophage-derived exosomes alleviates asthma progression through inhibiting the FGF1/MAPK/STAT1 axis

Exosomes: a new perspective in EGFR-mutated lung cancer

miR-365 (microRNA): Potential Biomarker in Oral Squamous Cell Carcinoma Exosomes and Extracellular Vesicles

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