The VEGF protein levels, miR-101-3p, and miR-122-5p are dysregulated in plasma from adolescents with major depression

Krivosova, Michaela; Adamcakova, Jana; Kaadt, Erik; Mumm, Birgitte; Dvorska, Dana; Braný, Dušan; Daňková, Zuzana; Dohál, Matúš; Samec, Marek; Ferencova, Nikola; Tonhajzerová, Ingrid; Ondrejka, Igor; Hrtanek, Igor; Hutka, Peter; Oppa, Miloslav; Mokrý, Juraj; Elfving, Betina

doi:10.1016/j.jad.2023.04.094

Cited by 3 publications

(2 citation statements)

References 51 publications

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“…miR-101-3p, a widely present miRNA in the human body, has been implicated in the development of various diseases including lung cancer (Dong et al 2023), diabetes mellitus , and acute gouty arthritis (Shao et al 2023). In a study investigating vascular growth factor expression in blood samples of depressed adolescents, miR-101-3p was found to be signi cantly upregulated in depressed patients (Krivosova et al 2023). Moreover, its target vascular growth factor also showed high expression levels.…”

Section: Discussionmentioning

confidence: 99%

Peripheral blood miRNA expression in patients with essential hypertension in the Han Chinese population in Hefei, China

Cheng,

Yang,

et al. 2024

Preprint

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Background. Primary hypertension is a significant risk factor for cardiovascular diseases. However, the pathogenesis of primary hypertension involves multiple biological processes, including the nervous system, circulatory system, endocrine system, and more. Despite extensive research, there is no clear understanding of the regulatory mechanism underlying its pathogenesis. In recent years, miRNAs have gained attention as a regulatory factor capable of modulating the expression of related molecules through gene silencing. Therefore, exploring differentially expressed miRNAs in patients with essential hypertension (EH) may offer a novel approach for future diagnosis and treatment of EH. Methodology. This study included a total of twenty Han Chinese population samples from Hefei, China. The samples consisted of 10 healthy individuals and 10 patients with essential hypertension (EH). Statistical analysis was conducted to analyze the general information of the two sample groups. High-throughput sequencing and base identification were performed to obtain the original sequencing sequences. These sequences were then annotated using various databases including Rfam, cDNA sequences, species repetitive sequences library, and miRBase database. The number of miRNA species contained in the samples was measured. Next, TPM values were calculated to determine the expression level of each miRNA. The bioinformatics of the differentiated miRNAs were analyzed using the OECloud tool, and RPM values were calculated. Furthermore, the reliability of the expression was analyzed by calculating the area under the Roc curve using the OECloud tools. Results. Statistical analysis revealed no significant differences between the two samples in terms of age distribution, gender composition, smoking history, and alcohol consumption history (P > 0.05). However, there was a notable presence of family genetic history and high BMI in the EH population (P < 0.05). The sequencing results identified a total of 245 miRNAs, out of which 16 miRNAs exhibited differential expression. Among the highly expressed miRNAs were let-7d-5p, miR-101-3p, miR-122-5p, miR-122b-3p, miR-192-5p, and miR-6722-3p. On the other hand, the lowly expressed miRNAs included miR-103a-3p, miR-16-5p, miR-181a-2-3p, miR-200a-3p, miR-200b-3p, miR-200c-3p, miR-221-3p, miR-30d-5p, miR-342-5p, and miR-543. Conclusion. This study initially identified 16 miRNAs that are aberrantly expressed and function in various processes associated with the onset and progression of essential hypertension. These miRNAs have the potential to be targeted for future diagnosis and treatment of EH. However, further samples are required to provide additional support for this study.

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Section: Discussionmentioning

confidence: 99%

Peripheral blood miRNA expression in patients with essential hypertension in the Han Chinese population in Hefei, China

Cheng,

Yang,

et al. 2024

Preprint

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“…Secondly, miRNA in plasma also makes a big difference. Plasma from adolescents exhibiting significant depressive symptoms demonstrates dysregulated levels of the VEGF protein, miR-101-3p, and miR--5p [ 76 ]. Jia Hu and colleagues found that post-stroke depression was more prevalent in patients with higher plasma levels of miR-22.…”

Section: Mirnamentioning

confidence: 99%