The vegetalizing factor belongs to a family of mesoderm-inducing proteins related to erythroid differentiation factor

Asashima, Makoto; Nakano, Hiroshi; Uchiyama, Hideho; Davids, Michael; Plessow, Sigrun; Loppnow-Blinde, Beate; Hoppe, Peter C.; Dau, H.; Tiedemann, Heinz

doi:10.1007/bf01135742

Cited by 48 publications

(20 citation statements)

References 13 publications

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“…STAT proteins may therefore not be involved in mediating the activin A growth inhibitory signal in this system. Activin A was ®rst studied as an inducer of follicle stimulating hormone (FSH) release (Vale et al, 1986;Ling et al, 1986), and was later found to be a pleiotropic cytokine exhibiting a variety of di erent functions (Murata et al, 1988;Fujimoto et al, 1991;Asashima et al, 1990;Smith et al, 1990;van den Eijnder et al, 1990;Thomsen et al, 1990;Matzuk et al, 1995;Zipori et al, 1986;Brosh et al, 1995). Included among its biological functions are also induction of growth inhibition and cell death.…”

Section: Discussionmentioning

confidence: 99%

Involvement of p21WAF1/Cip1, CDK4 and Rb in activin A mediated signaling leading to hepatoma cell growth inhibition

1997

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Section: Discussionmentioning

confidence: 99%

Involvement of p21WAF1/Cip1, CDK4 and Rb in activin A mediated signaling leading to hepatoma cell growth inhibition

1997

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“…Activin had long been known to have powerful mesoderm-inducing activity in amphibian embryos (Albano et al, 1990;Asashima et al, 1990;Smith et al, 1990;Thomsen et al, 1990), but its role in normal development was unclear until MOs were used to inhibit its effects (Piepenburg et al, 2004). In the course of these experiments, a MO designed to inhibit translation of activin B caused defects in development, as well as the dose-dependent downregulation of several mesodermal markers.…”

Section: Reviewmentioning

confidence: 99%

Controlling morpholino experiments: don't stop making antisense

2008

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One of the most significant problems facing developmental biologists who do not work on an organism with welldeveloped genetics -and even for some who do -is how to inhibit the action of a gene of interest during development so as to learn about its normal biological function. A widely adopted approach is to use antisense technologies, and especially morpholino antisense oligonucleotides. In this article, we review the use of such reagents and present examples of how they have provided insights into developmental mechanisms. We also discuss how the use of morpholinos can lead to misleading results, including off-target effects, and we suggest controls that will allow researchers to interpret morpholino experiments correctly.

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“…Two distinct pathways are involved in convergence extension and gastrulation movements in Xenopus: the activin/Nodal TGF-beta pathway (Asashima et al, 1990;Smith et al, 1990;Osada and Wright, 1999;Reissmann et al, 2001), and the Xnr3/FGF pathway (Yokota et al, 2003). Previous studies using a dominant-negative form of Dvl, Xdd1, suggested that Dvl is required downstream of Xnr3 for convergence extension movements (Yokota et al, 2003).…”

Section: Maternal Dvl2 and 3 Are Required For Convergence Extension Mmentioning

confidence: 99%

The functions of maternal Dishevelled 2 and 3 in the Early Xenopus embryo

Tadjuidje¹,

Cha²,

Louza³

et al. 2011

Developmental Dynamics

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Of the three Dishevelled (Dvl) genes, only Dvl2 and Dvl3 are maternally encoded in the frog, Xenopus laevis. We show here by loss of function analysis that single depletion of either Dvl2 or Dvl3 from the oocyte causes the same embryonic phenotype. We find that the effects of loss of function of Dvl2 and 3 together are additive, and that the proteins physically interact, suggesting that both are required in the same complex. We show that maternal Dvl2 and 3 are required for convergence extension movements downstream of the dorsally localized signaling pathway activated by Xnr3, but not downstream of the pathway activated by activin. Also, depletion of maternal Dvl2 and 3 mRNAs causes the up-regulation of a subset of zygotic ectodermal genes, including Foxi1e, with surprisingly no significant effect on the canonical Wnt direct target genes Siamois and Xnr3. We suggest that the likely reason for continued expression of the Wnt target genes in Dvl2/3-depleted embryos is that maternal Dvl mRNA depletion is insufficient to deplete stored punctae of Dvl protein in the oocyte cortex, which may transduce dorsal signaling after fertilization.

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The vegetalizing factor belongs to a family of mesoderm-inducing proteins related to erythroid differentiation factor

Cited by 48 publications

References 13 publications

Involvement of p21WAF1/Cip1, CDK4 and Rb in activin A mediated signaling leading to hepatoma cell growth inhibition

Involvement of p21WAF1/Cip1, CDK4 and Rb in activin A mediated signaling leading to hepatoma cell growth inhibition

Controlling morpholino experiments: don't stop making antisense

The functions of maternal Dishevelled 2 and 3 in the Early Xenopus embryo

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