The vascularization of vein-grafts

Wyatt, A P; Rothnie, N. G.; Taylor, G. W.

doi:10.1002/bjs.1800510522

Cited by 70 publications

(22 citation statements)

References 5 publications

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“…Another explanation is the ability of pre-existing ECs within the construct to activate an angiogenic response by secreting substantive growth factors, such as VEGF and bFGF [38]. Results similar to those described here were observed during graft bypass surgery, suggesting that vascularized TEBVs may react to revascularization in vivo in a manner comparable to that of a vein graft with vasa vasorum [39]. L'Heureux et al [31] reported observation of graft integration and in vivo formation of a neocapillary network without a pre-existing microvascular network only at 3 months after TEBV implantation.…”

Section: Discussionsupporting

confidence: 86%

Rapid vascularization of tissue-engineered vascular grafts in vivo by endothelial cells in co-culture with smooth muscle cells

Wang

et al. 2012

J Mater Sci: Mater Med

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A major challenge facing the development of tissue-engineered vascular grafts (TEVGs), promising living replacements for diseased vascular structures, is enhancing angiogenesis. To promote rapid vascularization, endothelial cells (ECs) were co-cultured with smooth muscle cells (SMCs) in decellularized small intestinal submucosa scaffolds to regenerate angiogenic-TEVGs (A-TEVGs). Observation of the A-TEVGs at 1 month post-implantation revealed that a rich network of neocapillaries lining the blood vessel wall had developed; that the ECs of the neovasculatures had been derived from previously seeded ECs and later invading ECs of the host's vascular bed; that tissue vascularization had not significantly impaired mechanical properties; and that the maximal tensile strength of the A-TEVGs was of the same order of magnitude as that of native porcine femoral arteries. These results indicate that of the co-culturing of ECs with SMCs could enhance vascularization of TEVGs in vivo, possibly increasing graft perfusion and host integration.

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Section: Discussionsupporting

confidence: 86%

Rapid vascularization of tissue-engineered vascular grafts in vivo by endothelial cells in co-culture with smooth muscle cells

Wang

et al. 2012

J Mater Sci: Mater Med

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“…These observations could be explained by the fact that inosculation allows nutrients to flow within the tissue-engineered construct with a capillary network, in comparison to the nonvascularized construct where passive diffusion is the only mean of transportation for oxygen and nutrients. Similar results were observed with arterial bypasses using vein graft by Wyatt et al, 29 suggesting that vascularized TEBV would react to revascularization in a comparable manner to vein grafts that possess a vasa vasorum.…”

Section: Discussionsupporting

confidence: 82%

Tissue-Engineered Vascular Adventitia with Vasa Vasorum Improves Graft Integration and Vascularization Through Inosculation

Guillemette

Gauvin

Perron

et al. 2010

Tissue Engineering Part A

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Tissue-engineered blood vessel is one of the most promising living substitutes for coronary and peripheral artery bypass graft surgery. However, one of the main limitations in tissue engineering is vascularization of the construct before implantation. Such a vascularization could play an important role in graft perfusion and host integration of tissue-engineered vascular adventitia. Using our self-assembly approach, we developed a method to vascularize tissue-engineered blood vessel constructs by coculturing endothelial cells in a fibroblast-laden tissue sheet. After subcutaneous implantation, enhancement of graft integration within the surrounding environment was noted after 48 h and an important improvement in blood circulation of the grafted tissue at 1 week postimplantation. The distinctive branching structure of end arteries characterizing the in vivo adventitial vasa vasorum has also been observed in long-term postimplantation follow-up. After a 90-day implantation period, hybrid vessels containing human and mouse endothelial cells were still perfused. Characterization of the mechanical properties of both control and vascularized adventitia demonstrated that the ultimate tensile strength, modulus, and failure strain were in the same order of magnitude of a pig coronary artery. The addition of a vasa vasorum to the tissue-engineered adventitia did not influence the burst pressure of these constructs. Hence, the present results indicate a promising answer to the many challenges associated with the in vitro vascularization and in vivo integration of many different tissue-engineered substitutes.

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“…It has been shown that the nutrition of a bypass vein graft is maintained by 'plasmic' circulation during the first 48 hours and later by capillary circulation which is re-established by 72 hours (Wyatt, Rothnie, and Taylor, 1964). Since there are similarities between the behaviour of a vein graft and an arterial graft, their nutritional requirements may also be alike.…”

Section: Resultsmentioning

confidence: 99%

Extracranial-intracranial bypass grafts

Khodadad

1972

Journal of Neurology, Neurosurgery & Psychiatry

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SUMMARY An extracranial-intracranial bypass graft was established in 16 dogs. The graft was routed deep in the lateral pharyngeal space as opposed to the subcutaneous course in front of the ear, which may be hazardous. Of 10 common carotid-middle cerebral arterial bypass grafts three were still patent four and a half, six, and 11 months postoperatively. The remaining seven arterial and six venous bypass grafts were occluded either at the first angiogram 1 to 14 days after the operation or at the second angiogram 4 to 9 weeks later. The low patency rate in this experiment is attributed to the very small external diameter (average 0-8 mm) of the recipient artery, to donor-recipient discrepancy, to spasm and possibly oedema of the graft. To our knowledge this is the first report on patent extracranial-intracranial bypass grafts in a laboratory animal.

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The vascularization of vein-grafts

Cited by 70 publications

References 5 publications

Rapid vascularization of tissue-engineered vascular grafts in vivo by endothelial cells in co-culture with smooth muscle cells

Rapid vascularization of tissue-engineered vascular grafts in vivo by endothelial cells in co-culture with smooth muscle cells

Tissue-Engineered Vascular Adventitia with Vasa Vasorum Improves Graft Integration and Vascularization Through Inosculation

Extracranial-intracranial bypass grafts

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