The Vascular Wall: a Plastic Hub of Activity in Cardiovascular Homeostasis and Disease

Awgulewitsch, Cassandra P.; Trinh, Linh T.; Hatzopoulos, Antonis K.

doi:10.1007/s11886-017-0861-y

Cited by 5 publications

(4 citation statements)

References 107 publications

(88 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The current hypotheses about the angiogenic mechanism of action of SVF involve myeloid, immune cells34 and vascular wall resident stem/progenitor cells,35 36 but highlight the major role of cooperation between MSC and EPC. The density of the vascular networks formed by the synergistic dual-cell system is many-fold higher than that found in implants containing either EPC or MSC alone 37.…”

Section: Discussionmentioning

confidence: 99%

Molecular profile and proangiogenic activity of the adipose-derived stromal vascular fraction used as an autologous innovative medicinal product in patients with systemic sclerosis

et al. 2019

View full text Add to dashboard Cite

ObjectiveThe autologous stromal vascular fraction (SVF) from adipose tissue is an alternative to cultured adipose-derived stem cells for use in regenerative medicine and represents a promising therapy for vasculopathy and hand disability in systemic sclerosis (SSc). However, the bioactivity of autologous SVF is not documented in this disease context. This study aimed to compare the molecular and functional profiles of the SVF-based medicinal product obtained from SSc and healthy subjects.MethodsGood manufacturing practice (GMP)-grade SVF from 24 patients with SSc and 12 healthy donors (HD) was analysed by flow cytometry to compare the distribution of the CD45− and CD45+ haematopoietic cell subsets. The ability of SVF to form a vascular network was assessed using Matrigel in vivo assay. The transcriptomic and secretory profiles of the SSc-SVF were assessed by RNA sequencing and multiplex analysis, respectively, and were compared with the HD-SVF.ResultsThe distribution of the leucocyte, endothelial, stromal, pericyte and transitional cell subsets was similar for SSc-SVF and HD-SVF. SSc-SVF retained its vasculogenic capacity, but the density of neovessels formed in SVF-loaded Matrigel implanted in nude mice was slightly decreased compared with HD-SVF. SSc-SVF displayed a differential molecular signature reflecting deregulation of angiogenesis, endothelial activation and fibrosis.ConclusionsOur study provides the first evidence that SSc does not compromise the vascular repair capacity of SVF, supporting its use as an innovative autologous biotherapy. The characterisation of the specific SSc-SVF molecular profile provides new perspectives for delineating markers of the potency of SVF and its targets for the treatment of SSc.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular profile and proangiogenic activity of the adipose-derived stromal vascular fraction used as an autologous innovative medicinal product in patients with systemic sclerosis

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Actually, not only do ECs serve as gatekeepers of organ homeostasis [106], but they are also essential to maintain the function of arterioles in providing nutrients to cancer cells [19,20,107], including CSCs. EC differentiation likely plays a key role in tumor arteriogenesis [20,27,55,91,108] in that arteriolar ECs may recruit SMCs to form arterioles and promote tumor progression by serving as an arteriolar niche for CSC maintenance and self-renewal.…”

Section: Tumor Arteriogenesis: Potential Target In Pnetsmentioning

confidence: 99%

Heterogeneity of Vascular Endothelial Cells, De Novo Arteriogenesis and Therapeutic Implications in Pancreatic Neuroendocrine Tumors

Ren

Rose

Liu

et al. 2019

JCM

View full text Add to dashboard Cite

Arteriogenesis supplies oxygen and nutrients in the tumor microenvironment (TME), which may play an important role in tumor growth and metastasis. Pancreatic neuroendocrine tumors (pNETs) are the second most common pancreatic malignancy and are frequently metastatic on presentation. Nearly a third of pNETs secrete bioactive substances causing debilitating symptoms. Current treatment options for metastatic pNETs are limited. Importantly, these tumors are highly vascularized and heterogeneous neoplasms, in which the heterogeneity of vascular endothelial cells (ECs) and de novo arteriogenesis may be critical for their progression. Current anti-angiogenetic targeted treatments have not shown substantial clinical benefits, and they are poorly tolerated. This review article describes EC heterogeneity and heterogeneous tumor-associated ECs (TAECs) in the TME and emphasizes the concept of de novo arteriogenesis in the TME. The authors also emphasize the challenges of current antiangiogenic therapy in pNETs and discuss the potential of tumor arteriogenesis as a novel therapeutic target. Finally, the authors prospect the clinical potential of targeting the FoxO1-CD36-Notch pathway that is associated with both pNET progression and arteriogenesis and provide insights into the clinical implications of targeting plasticity of cancer stem cells (CSCs) and vascular niche, particularly the arteriolar niche within the TME in pNETs, which will also provide insights into other types of cancer, including breast cancer, lung cancer, and malignant melanoma.

show abstract

“…Endothelial cells therefore exhibit a wide range of plasticity, from alterations in the expression of various membrane receptors and adhesion molecules, changes in their morphology and shape, their mitogenic potential and even their potential to migrate or transit into different cell types (endothelial-to-mesenchymal transition) [3,11].…”

Section: Endothelial Heterogeneitymentioning

confidence: 99%

“…An important communication exists between the perivascular adipose tissue (PVAT), endothelial cells, and VSMCs: PVAT secretes a number of adipokines (tumor necrosis factor, TNFα, interleukin-6, IL-6, resistin, irisin) with various pro-and antiatherogenic properties [8,11]. The level of adipocytokines has been suggested as an independent predictor of endothelial dysfunction in healthy subjects [17].…”

Section: Gap Junctions In Turn Enable a Direct Transmission Of Electrmentioning

confidence: 99%

Endothelium at a Glance

Lenasi¹

2018

Endothelial Dysfunction - Old Concepts and New Challenges

View full text Add to dashboard Cite

Exposed to the blood milieu and variable hemodynamic forces, endothelial cells of different vessels exhibit significant heterogeneity, directing also the variety of endothelial functions. Endothelial cells are actively involved in many physiological processes, including vascular tone regulation, fluid filtration and reabsorption processes, maintenance of blood fluidity and proper hemostasis, leucocyte trafficking, tissue repair, and angiogenesis; accordingly, healthy endothelium is crucial for vascular homeostasis. On the other hand, many exo-and endogenous harmful factors can cause endothelial dysfunction, associated with inflammation, thrombosis, pathological vascular wall remodeling, and predisposing to the development of cardiovascular and other diseases. In order to design accurate clinical and pharmacological strategies to postpone or ameliorate endothelial dysfunction, endothelial dysfunction should firstly be recognized. Therefore, understanding endothelial physiology is crucial for clinical measures to be timely taken. The review briefly outlines some basic concepts of endothelial structure and function, focusing on endothelial barrier function and endothelium-dependent vasodilation, and addressing some potential therapeutic targets. Additional specific concepts of endothelial (dys)function, with particular emphasis on its involvement in inflammation, hemostasis, and its (mal)adaptation to environmental challenges are extensively described in the following book chapters.

show abstract

The Vascular Wall: a Plastic Hub of Activity in Cardiovascular Homeostasis and Disease

Cited by 5 publications

References 107 publications

Molecular profile and proangiogenic activity of the adipose-derived stromal vascular fraction used as an autologous innovative medicinal product in patients with systemic sclerosis

Molecular profile and proangiogenic activity of the adipose-derived stromal vascular fraction used as an autologous innovative medicinal product in patients with systemic sclerosis

Heterogeneity of Vascular Endothelial Cells, De Novo Arteriogenesis and Therapeutic Implications in Pancreatic Neuroendocrine Tumors

Endothelium at a Glance

Contact Info

Product

Resources

About