The variations of antibiotics and antibiotic resistance genes in two subtropical large river basins of south China: Anthropogenic impacts and environmental risks

Gao, Fangliang; He, Liang-Ying; Hu, Li‐Xin; Yang, Yuanyuan; He, Lu-Xi; Bai, Hong; Liu, You-Sheng; Zhao, Jian‐Liang; Ying, Guang‐Guo

doi:10.1016/j.envpol.2022.119978

Cited by 16 publications

(2 citation statements)

References 46 publications

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“…Then, the wastes were used for agricultural fertilization or transported to treatment plants for centralized treatment. To this end, the water systems, soil and air had been contaminated by farming activities [7,10,32,33].…”

Section: Study Area and Sample Collectionmentioning

confidence: 99%

Swine farm groundwater is a hidden hotspot for antibiotic-resistant pathogenic Acinetobacter

Gao

Chen

et al. 2023

ISME Communications

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Acinetobacter is present in the livestock environment, but little is known about their antibiotic resistance and pathogenic species in the farm groundwater. Here we investigated antibiotic resistance of Acinetobacter in the swine farm groundwater (JZPG) and residential groundwater (JZG) of a swine farming village, in comparison to a nearby (3.5 km) non-farming village (WTG) using metagenomic and culture-based approaches. Results showed that the abundance of antibiotic resistome in some JZG and all JZPG (~3.4 copies/16S rRNA gene) was higher than that in WTG (~0.7 copies/16S rRNA gene), indicating the influence of farming activities on both groundwater types. Acinetobacter accounted for ~95.7% of the bacteria in JZG and JZPG, but only ~8.0% in WTG. They were potential hosts of ~95.6% of the resistome in farm affected groundwater, which includes 99 ARG subtypes against 23 antibiotic classes. These ARGs were associated with diverse intrinsic and acquired resistance mechanisms, and the predominant ARGs were tetracyclines and fluoroquinolones resistance genes. Metagenomic binning analysis elucidated that non-baumannii Acinetobacter including A. oleivorans, A. beijerinckii, A. seifertii, A. bereziniae and A. modestus might pose environmental risks because of multidrug resistance, pathogenicity and massive existence in the groundwater. Antibiotic susceptibility tests showed that the isolated strains were resistant to multiple antibiotics including sulfamethoxazole (resistance ratio: 96.2%), levofloxacin (42.5%), gatifloxacin (39.0%), ciprofloxacin (32.6%), tetracycline (32.0%), doxycycline (29.0%) and ampicillin (12.0%) as well as last-resort polymyxin B (31.7%), colistin (24.1%) and tigecycline (4.1%). The findings highlight potential prevalence of groundwater-borne antibiotic-resistant pathogenic Acinetobacter in the livestock environment.

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Section: Study Area and Sample Collectionmentioning

confidence: 99%

Swine farm groundwater is a hidden hotspot for antibiotic-resistant pathogenic Acinetobacter

Gao

Chen

et al. 2023

ISME Communications

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“…Due to its role in the geologic and hydrologic cycles, it acts as a significant circulation point for antibiotics in the ecosystem [ 8 , 9 ]. Particularly in areas with intensive livestock farming where antibiotics are routinely used, sediment in the watershed becomes a junction where both natural processes and anthropogenic activities converge, leading to a significant accumulation of these compounds [ 10 , 11 , 12 ]. In addition, the sediment ecosystem serves as a biodiverse habitat for innumerable bacterial communities, contributing significantly to maintaining ecological equilibrium through processes such as the cycling of nutrients and the degradation of complex organic matter [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Difference of Microbial Community in the Stream Adjacent to the Mixed Antibiotic Effluent Source

Kim,

Hong,

Kwon

et al. 2024

Toxics

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Released antibiotics from source to stream can influence bacterial communities and potentially alter the ecosystem. This research provides a comprehensive examination of the sources, distribution, and bacterial community dynamics associated with varied antibiotic release sources adjacent to the stream. The residual of antibiotics from different sources was determined, and the bacterial community structure was examined to reveal the differences in the bacteria community in the stream. The residual of antibiotics was quantified with liquid chromatography–tandem mass spectrometry (LC-MS/MS), and the Illumina MiSeq platform was utilized to sequence bacterial 16S rRNA genes, providing comprehensive insights into the bacterial community structure in the sediment across five different sites. Results indicated that the presence and distribution of antibiotics were significantly influenced by released sources. In the case of the bacterial community, the Proteobacteria and Firmicutes were the most dominant phyla in the sediment, and especially, the Firmicutes showed higher abundance in sites mostly affected by livestock sources. Additionally, livestock gut bacteria such as Clostridium saudiense, Proteiniclasticum ruminis, and Turicibacter sanguinis were prevalent in antibiotic-contaminated sites adjacent to livestock facilities. Overall, this study provides critical insights into the effect of antibiotic contamination by verifying the relationship between the occurrence of antibiotic residuals and the alteration in the bacterial community in the stream.

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The occurrence and abundance of antibiotic resistance genes in rivers of tropical islands: a case of Hainan Island, China

Wei

Gao

et al. 2023

Environ Sci Pollut Res

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The variations of antibiotics and antibiotic resistance genes in two subtropical large river basins of south China: Anthropogenic impacts and environmental risks

Cited by 16 publications

References 46 publications

Swine farm groundwater is a hidden hotspot for antibiotic-resistant pathogenic Acinetobacter

Swine farm groundwater is a hidden hotspot for antibiotic-resistant pathogenic Acinetobacter

Difference of Microbial Community in the Stream Adjacent to the Mixed Antibiotic Effluent Source

The occurrence and abundance of antibiotic resistance genes in rivers of tropical islands: a case of Hainan Island, China

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