The Value of Tubular Enzymes for Early Detection of Acute Kidney Injury After Liver Transplantation: An Observational Study

Santos, Clara; Marcelino, Paulo; Carvalho, T. F. de; Coelho, J.; Bispo, Miguel; Mourão, Luís; Perdigoto, Rui; Barroso, Eduardo

doi:10.1016/j.transproceed.2010.06.024

Cited by 10 publications

(5 citation statements)

References 16 publications

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“…In addition, this phenomenon has been correlated with a high susceptibility to secondary insult in animals postsepsis (5). This idea is strengthened by the observation that other pathologies linked to severe acute inflammation (25)(26)(27)(28)(29) are followed by a long-lasting improper immune response. Wen et al (30) demonstrated in the CLP model that dendritic cells from sepsissurviving animals are not immunologically competent because of the absence of capacity to produce cytokines, such as IL-12.…”

Section: Discussionmentioning

confidence: 92%

“…Previous studies have reported that urinary levels of LDH or yGT are efficient biomarkers for early diagnosis of acute tubular necrosis, because the urinary levels of these are increased before any decrease in GFR and development of kidney injury (23). Both enzymes are useful for the purpose of broad screening along the nephron; yGT displays accuracy and sensitivity for the detection of initial renal tubular lesions (23)(24)(25). In accordance with the reports, we observed that the CLP^^^+BSA group showed a significant increase in urinary LDH, yGT, and tubular apoptosis compared with the Sham,,j+SAL, CLP^^-I-SAL, or Sham2,j+BSA groups.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Sepsis-Surviving Mice Are More Susceptible to a Secondary Kidney Insult*

Portella

Silva-Filho²,

Landgraf³

et al. 2013

Critical Care Medicine

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Sepsis-Surviving Mice Are More Susceptible to a Secondary Kidney Insult*

Portella

Silva-Filho²,

Landgraf³

et al. 2013

Critical Care Medicine

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“…In the kidney, AAP is released into urine by damaged tubular cells during acute renal tubular necrosis, renal graft rejection, or nephrotoxic action of immunosuppressive drugs. [142][143][144] In an experimental cisplatin-induced AKI model in the rat, AAP showed good predictive accuracy for AKI with an AUC value of 0.89. 145 Lactate dehydrogenase (LDH) is a 140-kDa ubiquitous enzyme that catalyzes the anaerobic, nicotinamide adenine dinucleotide phosphate-dependent interconversion of pyruvate and lactate.…”

Section: Q26mentioning

confidence: 99%

Proteomics and Metabolomics for AKI Diagnosis

Marx

Metzger

Pejchinovski

et al. 2018

Seminars in Nephrology

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Acute kidney injury (AKI) is a severe and frequent condition in hospitalized patients. Currently, no efficient therapy of AKI is available. Therefore, efforts focus on early prevention and potentially early initiation of renal replacement therapy to improve the outcome in AKI. The detection of AKI in hospitalized patients implies the need for early, accurate, robust, and easily accessible biomarkers of AKI evolution and outcome prediction because only a narrow window exists to implement the earlier-described measures. Even more challenging is the multifactorial origin of AKI and the fact that the changes of molecular expression induced by AKI are difficult to distinguish from those of the diseases associated or causing AKI as shock or sepsis. During the past decade, a considerable number of protein biomarkers for AKI have been described and we expect from recent advances in the field of omics technologies that this number will increase further in the future and be extended to other sorts of biomolecules, such as RNAs, lipids, and metabolites. However, most of these biomarkers are poorly defined by their AKI-associated molecular context. In this review, we describe the state-of-the-art tissue and biofluid proteomic and metabolomic technologies and new bioinformatics approaches for proteomic and metabolomic pathway and molecular interaction analysis. In the second part of the review, we focus on AKI-associated proteomic and metabolomic biomarkers and briefly outline their pathophysiological context in AKI.

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“…However, more prominent tubular changes were observed, suggesting that GV sPLA 2 plays a more pronounced role in the function and integrity of the tubular compartment. Previous studies reported that urinary levels of LDH and γGT are linked to apoptosis of tubular renal cells and, consequently, early diagnosis of kidney disease [ 45 ]. Here, we verified intense leakage of LDH and γGT in the urine of Pla2g5 −/− mice, which suggests a potential role for GV sPLA 2 in the integrity of tubular cells.…”

Section: Discussionmentioning

confidence: 99%

Group V Secretory Phospholipase A2 Is Involved in Tubular Integrity and Sodium Handling in the Kidney

et al. 2016

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Group V (GV) phospholipase A2 (PLA2) is a member of the family of secreted PLA2 (sPLA2) enzymes. This enzyme has been identified in several organs, including the kidney. However, the physiologic role of GV sPLA2 in the maintenance of renal function remains unclear. We used mice lacking the gene encoding GV sPLA2 (Pla2g5−/−) and wild-type breeding pairs in the experiments. Mice were individually housed in metabolic cages and 48-h urine was collected for biochemical assays. Kidney samples were evaluated for glomerular morphology, renal fibrosis, and expression/activity of the (Na+ + K+)-ATPase α1 subunit. We observed that plasma creatinine levels were increased in Pla2g5−/− mice following by a decrease in creatinine clearance. The levels of urinary protein were higher in Pla2g5−/− mice than in the control group. Markers of tubular integrity and function such as γ-glutamyl transpeptidase, lactate dehydrogenase, and sodium excretion fraction (FENa+) were also increased in Pla2g5−/− mice. The increased FENa+ observed in Pla2g5−/− mice was correlated to alterations in cortical (Na+ + K+) ATPase activity/ expression. In addition, the kidney from Pla2g5−/− mice showed accumulation of matrix in corticomedullary glomeruli and tubulointerstitial fibrosis. These data suggest GV sPLA2 is involved in the maintenance of tubular cell function and integrity, promoting sodium retention through increased cortical (Na+ + K+)-ATPase expression and activity.

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The Value of Tubular Enzymes for Early Detection of Acute Kidney Injury After Liver Transplantation: An Observational Study

Cited by 10 publications

References 16 publications

Sepsis-Surviving Mice Are More Susceptible to a Secondary Kidney Insult*

Sepsis-Surviving Mice Are More Susceptible to a Secondary Kidney Insult*

Proteomics and Metabolomics for AKI Diagnosis

Group V Secretory Phospholipase A2 Is Involved in Tubular Integrity and Sodium Handling in the Kidney

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