The Value of the Nitric Oxide System in the Functioning of the Stomach in Norm and Pathology

Олещук, О. М.; CHORNOMYDZ, Andrii

doi:10.11603/mcch.2410-681x.2016.v0.i2.6679

Cited by 3 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…interaction with the superoxide anion O 2-with NO produces a highly active strong oxidant -peroxynitrite (ONOO -), which interacts with lipids, can cause peroxidation of the latter, and the formation of nitrotyrosine in interaction with tyrosine residues of proteins disrupts their functions. metabolism at all levels [14,15]. It is known that the formation of peroxynitrite is the main pathway of NO metabolism.…”

Section: Resultsmentioning

confidence: 99%

“…It is known that the formation of peroxynitrite is the main pathway of NO metabolism. Its amount depends in vivo on the relative entry of NO and superoxide anion into tissues, and the concentrations of both free radical compounds should be approximately the same [14,15]. There is evidence that peroxynitrite stimulates matrix metalloprotein kinase-8 (MMP-8), which plays an important role in tissue destruction and rearrangement both in physiological conditions and in the development of inflammation and infection.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, prolonged exposure to vascular smooth muscle cells has led to a loss of the latter's ability to respond normally to physiological stimuli, resulting in irreversible relaxation of myocytes. Such processes can lead to disruption of regional blood flow, which is a damaging factor in ulcerogenesis [14,15].…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Characteristics of Cytoprotective Effect on the Gastric Mucosa of Cryopreserved Placenta Extract Under Water-Immobilization Stress

Кошурба

Hladkykh

Chyzh

2022

AML

View full text Add to dashboard Cite

Various stressors are currently considered to play a significant role among the etiological factors for development of peptic ulcer disease. Along with psycho-emotional stressing experiences, the causes of formation of stress ulcers include major surgical interventions, thermal or mechanical injuries, severe blood loss, progressive syndrome of endogenous intoxication and more. Among patients in intensive care units, the incidence of stress ulcers is 5.0%, and in 30.0-50.0% of cases stress ulcers are complicated by bleeding and perforation. Aim. The research aims at characterizing the gastrocytoprotective effect of cryopreserved placenta extract (CEP) on the model of water-immobilization stress in rats according to macroscopic data and the results of biochemical study of homogenates of the gastric mucosa (GM). Materials and Methods. In vivo experimental studies were performed on 28 nonlinear laboratory male rats weighing 200-220 g. Stress-induced gastric ulcer was simulated under water-immobilization stress (WIS) in rats. WIS simulation according to the method of Takagi K.Y. et al. The activity of NO synthases (NOS) in GM was determined spectrophotometrically by the amount of oxidized NADPH+H+. Results and Discussion. The use of CEP, as well as esomeprazole, led to a weakening of GM ulcers. This was indicated by a statistically significant (p<0.05) decrease in ulcer index (UI) relative to the indicators of control rats by 9.8 and 3.3 times, respectively. At the same time, this indicator was three times lower on the background of preventive use of CEP than in rats treated with esomeprazole, respectively, antiulcer activity (AUA) was 96.4% and 69.2%. The study of the activity of constitutive (cNOS) and inducible (iNOS) isoforms of NOS showed that at the pathobiochemical level against the background of stress-induced ulcerogenesis in the tissues of the central nervous system there is a statistically significant (p<0.001) increase in iNOS activity in 5.2 times was 0.89±0.01 NADPH2/min´g protein. According to the degree of modulation of the activity of both total NOS and its individual isoforms, the study showed that preventive five-day administration of esomeprazole is inferior to the effectiveness of CEP. Thus, the activity of total NOS in rats administered esomeprazole was statistically significantly (p<0.001) decreased by only 17.1%, while the activity of this enzyme decreased (p<0.001) by 35.8% due to the use of CEP. Conclusions. Prophylactic five-day administration of CEP modulated the activity of the NOS system in the central nervous system, which in a macroscopic study appeared statistically significant (p<0.05) reduction in UI relative to rats in the control group by 9.8 times. Thus, NOS activity statistically significantly (p<0.001) decreased by 35.8%, and iNOS activity statistically significantly (p<0.001) decreased by 58.4% relative to the control animals. CEP under the prophylactic regimen showed more pronounced anti-ulcer activity than esomeprazole, which was 96.4% and 69.2%, respectively.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Characteristics of Cytoprotective Effect on the Gastric Mucosa of Cryopreserved Placenta Extract Under Water-Immobilization Stress

Кошурба

Hladkykh

Chyzh

2022

AML

View full text Add to dashboard Cite

show abstract

Gender-Specific Differences of Oxidative Processes in the Population of Circulating Neutrophils of Rats in a Setting of Prolonged Administration of Monosodium Glutamate

Krynytska

Rutska

2019

Romanian Journal of Diabetes Nutrition and Metabolic Diseases

View full text Add to dashboard Cite

Background and aims:Monosodium salt of glutamic acid (MSG) is one of the most common food additives. The aim of study was to assess, in gender-specific terms, how prolonged administration of MSG effects on reactive oxygen and nitrogen species formation and the apoptotic/necrotic processes in the population of rats circulating neutrophils. Material and methods: Experimental studies were conducted on 32 mature white rats. MSG was administered intragastrical at a dose of 30 mg/kg body weight for 30 days. The analysis of cell samples to determine neutrophils with overproduction of reactive oxygen species (ROS) and signs of apoptosis\necrosis was evaluated with flow laser cytometry method. The total nitric oxide synthase (NOS) activity was determined by monitoring the rate of conversion of L-arginine into citrulline. The total quantity of NO metabolites was assessed by evaluating of nitrite and nitrate ions. Results: We found a significant increase in generation of ROS, intensification of nitroxydergic processes, an increase in the percentage of apoptotic neutrophils and no changes in the percentage of necrotic neutrophils. Conclusions: We observed activation of oxidative and nitroxydergic processes in rats with prolonged administration of MSG, which initiate apoptosis. In gender-specific terms, a more pronounced changes were seen in male rats. key words: monosodium glutamate, reactive oxygen species, nitric oxide, NO-synthase; apoptosis.

show abstract

Activity of Nitro-Oxidative Processes in Pregnant Women With Idiopathic Arterial Hypotension

Markin

Popovych

2022

AML

View full text Add to dashboard Cite

Primary violation of vascular tone and reactivity is considered the main hemodynamic link in the pathogenesis of idiopathic arterial hypotension (IAH). Two gaseous messengers - nitric oxide (NO) and hydrogen sulfide (H2S) play an important role in the regulation of vascular tone. The research aims at determining the state of the NO system and the level of H2S in women with IAH and their effect on the uteroplacental blood flow. Material and Methods. A number of 20 pregnant women with IAH at 40-41 weeks of gestation were examined (group I); the second group included 20 pregnant women with normotony at 39-40 weeks of gestation. The concentration of the complex of nitrates and nitrites was determined by the reaction of formation of a colored complex by Griss reagent. The content and production of peroxynitrite in alkali and alkaline earth metals were determined according to the procedure described by Vanuffelen et al. Detection of hydrogen sulfide content in blood plasma was performed by the method of Dombkowski et al. Studies of uteroplacental blood flow were performed on ultrasonic diagnostic device "SA-8000 EX" using color Doppler mapping and pulse mapping dopplerometry of the blood flow in the uterine arteries (UA). The assessment of blood flow velocity curves was carried out by determining the pulsation index (PI) in both arteries taking into account the average value. Results. The results of the study showed that an average level of nitrites and nitrates in pregnant women with IAH was 37.0% higher than in women with normotension, and peroxynitrite values were 2.4 times higher, respectively. In contrast, the concentration of H2S in pregnant women with IAH was almost 1.4 times lower, than in pregnant women with normal blood pressure. Doppler study of blood flow indices in UA showed a moderate increase in PI in 28.0% of pregnant women with IAH. Thus, indices of vascular resistance in UA of pregnant women with IAH were higher, on average, by 19.0% than in pregnant women with normotension. Conclusions. High levels of nitrites and nitrates in pregnant women with IAH form the basis for the development of nitrosatous stress, which is accompanied by increasing concentration of highly toxic peroxynitrite. Significant increase in peroxynitrite is one of the causes of endothelial dysfunction. In pregnant women with IAH due to the low content of H2S - its vasoconstrictive effects are prevalent, which may indicate an indirect interaction between both of these systems of vasoregulation.

show abstract

The Value of the Nitric Oxide System in the Functioning of the Stomach in Norm and Pathology

Cited by 3 publications

References 0 publications

Characteristics of Cytoprotective Effect on the Gastric Mucosa of Cryopreserved Placenta Extract Under Water-Immobilization Stress

Characteristics of Cytoprotective Effect on the Gastric Mucosa of Cryopreserved Placenta Extract Under Water-Immobilization Stress

Gender-Specific Differences of Oxidative Processes in the Population of Circulating Neutrophils of Rats in a Setting of Prolonged Administration of Monosodium Glutamate

Activity of Nitro-Oxidative Processes in Pregnant Women With Idiopathic Arterial Hypotension

Contact Info

Product

Resources

About