The value of reverse banding in detecting bone marrow chromosomal abnormalities: Translocation between chromosomes 1, 9, and 22 in a case of chronic myelogenous leukemia (CML)

Abstract

A case of chronic myelogenous leukemia (CML) with complex chromosomal abnormalities is reported. Conventional staining techniques indicated incorrectly that the Ph1 chromosome was not present. These studies showed a 46,XY,-1,+C karyotype in all bone marrow cells. Employing RFA (R bands by fluorescence using acridine orange) technique it was clear that the part of the long arm of chromosome 1 (1q23 leads to qter) was missing the tip of the long arm chromosome 22 (band q22) was translocated to it. The missing long arm of chromosome 1 was translocated to the long arm of chromosome 9. Furthermore, there was a break at band 9q22 and the whole terminal part was lost. The value of RFA technique is discussed.

Heteromorphisms of the Philadelphia (Ph1) Chromosome in Patients with Chronic Myelogenous Leukaemia (CML). I. CLASSIFICATION AND CLINICAL SIGNIFICANCE

“…Two slides from each patient were stained with orcein and 20 cells were counted. Sequential QFQ (Q-bands by fluorescence using quinacrine) and RFA (R-bands by fluorescence using acridine orange; nomenclature suggested by Paris Conference (1Y71), Supplement (1975)) technique was performed as described previously (Verma & Lubs, 1975Verma & Dosik, 1976). RFA banded chromosomes were photographed on Kodachrome 64 colour transparency film using a Zeiss photomicroscope 11.…”

Fusion of platelet-derived growth factor receptor β to CEV14 gene in chronic myelomonocytic leukemia: A case report and review of the literature

“…Termination of culture was performed following culturing in colchamine for 1 h, and cells were subsequently fixed in a solution of methanol and glacial acetic acid (methanol:glacial acetic acid, 3:1). Following fixing, cells were collected and chromosome karyotype was analyzed after conventional reverse-banding (9). The remaining cells were stored in fresh fixative and preserved at 20˚C.…”