The value of pronuclear scoring for the success of IVF and ICSI-cycles

Ludwig, A. K.; Werner, S. G.; Diedrich, K.; Nitz, Barbara; Ludwig, Michael

doi:10.1007/s00404-005-0102-2

Cited by 8 publications

(3 citation statements)

References 30 publications

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“…PN scoring was postulated to provide predictive value of embryo development, even of the chromosomal constitution and implantation potential of the embryo (Gianaroli et al, 2007;Montag and van der Ven, 2001;Salumets et al, 2003;Scott and Smith, 1998;Scott et al, 2000;Tesarik and Greco, 1999;Wittemer et al, 2000). Therefore, PN scoring is widely used to predict embryo fate, but is of course, the subject of a controversial debate (James et al, 2006;Ludwig et al, 2006;Payne et al, 2005). Many investigators have found a relationship between PN scoring and improved embryo development/blastocyst formation (Balaban et al, 2001;Nagy et al, 2003;Rienzi et al, 2002;Zollner et al, 2002) and/or increased pregnancy and implantation potential (Montag and van der Ven, 2001;Wittemer et al, 2000;reviewed by Zollner et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Transfer of blastocysts with deviant morphological and morphokinetic parameters at early stages of in-vitro development: a case series

Stecher

Vanderzwalmen

Zintz

et al. 2014

Reproductive BioMedicine Online

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Time-lapse imaging is increasingly applied as an adjunct to reproductive medicine. The gained information of the morphological and morphokinetic variables before the onset of transcription are supposed to be good predictors for the selection of the best embryo for transfer and are often seen in line with clinical outcomes. This retrospective case series investigated the outcome of transferred blastocysts that did not fulfil the proposed embryo scores at early cleavage or at later stages of development. The observations were made by time-lapse imaging. This study reports the birth of 16 healthy children after day-5 blastocyst transfer, of which at least one of the transferred embryos originated from deviant morphology and/or kinetic cleavage patterns. This case series suggests that some blastocysts derived from embryos with poor conventional morphological score and/or suboptimal morphokinetics can be successfully transferred and might result in live births. Such results might raise awareness that discarding embryos based only on early events is not a suitable approach to give patients the chance to conceive. In conclusion, to date only the transfer of viable embryos after culturing them until day 5 guarantees optimal embryo selection and helps to prevent embryo wastage.

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Section: Introductionmentioning

confidence: 99%

Transfer of blastocysts with deviant morphological and morphokinetic parameters at early stages of in-vitro development: a case series

Stecher

Vanderzwalmen

Zintz

et al. 2014

Reproductive BioMedicine Online

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show abstract

“…The insemination of the oocytes was performed using standard procedures for IVF or ICSI as previously described elsewhere (21)(22)(23) and was performed 39 to 42 hours after the hCG injection. Fertilization was confirmed 16 to 20 hours after oocyte retrieval by the presence of two distinct pronuclei and polar bodies under the inverted microscope.…”

Section: Fertilization Pronuclear Scoring and Embryo Culturementioning

confidence: 99%

Evaluation of in vitro fertilization outcomes using interleukin-8 in culture medium of human preimplantation embryos

Huang

Congrong

Wei

et al. 2017

Fertility and Sterility

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“…Pattern 0 [6] and Z1 or Z2 [10] have been associated with an increase in implantation and pregnancy rates [7,8,[11][12][13][14][15]. Recently however, a number of investigators have not been able to demonstrate an association between PN scoring and improved ART outcomes [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Embryo quality but not pronuclear score is associated with clinical pregnancy following IVF

Berger

Zapantis

Merhi

et al. 2014

J Assist Reprod Genet

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Purpose The association between pronuclear (PN) scoring of embryos from assisted reproductive technology (ART) and clinical pregnancy remains controversial. We hypothesized that embryos with PNs scored on the day of fertilization check offer better embryo selection on day 3 and higher CPR compared to non-PN scored embryos. Methods Patients (19-46 years) undergoing IVF/ICSI cycles at Montefiore's Institute for Reproductive Medicine and Health between January 2006 and December 2009 were included in our study. We analyzed fresh day 3 cycles only with autologous oocytes and partner's fresh sperm (n=344). A total of 1,899 embryos were included. We compared CPR from non-PN scored embryos (Group 1, n=835) with PN scored embryos (Group 2, n=1,064). Composite scores by patient were developed based on embryo disposition. We also assessed traditional embryo grading derived from cell number, fragmentation and cell symmetry. Data analysis included chi square and t test to determine if PN scoring was associated with improved CPR, and to compare the additional variables.Results CPR between Group 1 and Group 2 were not different (p=0.91). CPR was significantly associated with female age, number of mature oocytes retrieved, number of day 3 embryos and grade of embryos transferred on day 3 (p<0.05). Conclusion PN scoring was not associated with improved CPR in day 3 embryo transfers. Mean grade of transferred embryos continues to be a well-established, independent predictor of CPR. We conclude that further refinement of embryo grading by PN scoring is not beneficial.

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The value of pronuclear scoring for the success of IVF and ICSI-cycles

Abstract: Benefit from pronuclear scoring seems to be small. Apparently, experienced biologists are able to select "good-quality" pronuclear oocytes in the same way they would do after scoring. However, the results might be biased by differences between the groups.

Cited by 8 publications

References 30 publications

Transfer of blastocysts with deviant morphological and morphokinetic parameters at early stages of in-vitro development: a case series

Transfer of blastocysts with deviant morphological and morphokinetic parameters at early stages of in-vitro development: a case series

Evaluation of in vitro fertilization outcomes using interleukin-8 in culture medium of human preimplantation embryos

Embryo quality but not pronuclear score is associated with clinical pregnancy following IVF

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