The value of phenol red and chromic chloride as nonabsorbable gastric indicators

Rj, Clarke; Ja, Williams

doi:10.1136/gut.12.5.389

Cited by 8 publications

(3 citation statements)

References 5 publications

(4 reference statements)

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“…24 Basic research in the GI motility field then moved to more invasive techniques for studying rodents, mostly those involving the gavage of markers and subsequent sacrifice of the animal to determine gastric emptying and/or upper GI transit. 25,26 Other techniques allow for whole-gut transit to be determined in a non-invasive way, but these may be highly time-consuming or require isolation of the animals. For example, a colored marker or a small metallic bead is placed in the stomach and the time taken for the first colored fecal pellet or the bead to be expelled is recorded.…”

Section: Discussionmentioning

confidence: 99%

X‐ray analysis of gastrointestinal motility in conscious mice. Effects of morphine and comparison with rats

Girón

Pérez-García

Abalo

2015

Neurogastroenterology Motil

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Background Non‐invasive methods to study gastrointestinal (GI) motility are of high interest, particularly in chronic studies. Amongst these, radiographic techniques after contrast intragastric administration may offer many advantages. In previous studies, we have successfully and reproducibly applied these techniques together with a semiquantitative analysis method to characterize the effect of different drugs, acutely or repeatedly administered in rat models, but we have never before used these techniques in mice. These are very convenient in basic research. Our aim was to determine if our method is also valid in mice. Additionally, we determined the effect of morphine on GI motor function in both species. Methods Animals received an intraperitoneal administration of morphine (at 10 and 5 mg/kg for rats and mice, respectively). Twenty min later, barium contrast (at 2 g/mL) was gavaged (2.5 and 0.4 mL for rats and mice respectively) and serial X‐rays were obtained 0–8 h after contrast. X‐rays were analyzed as previously described, using a semiquantitative score to build motility curves for each GI region. Key Results Motility was much faster in mice than in rats for all GI regions. Morphine at the doses used significantly depressed motility in both species to a similar extent if the whole gut or the upper GI regions (stomach, small intestine) were considered, although its effect seemed to be more intense in the lower GI regions (caecum, colorectum) in rats than in mice. Conclusions & Inferences We have validated our X‐rays method for its use in mice.

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Section: Discussionmentioning

confidence: 99%

X‐ray analysis of gastrointestinal motility in conscious mice. Effects of morphine and comparison with rats

Girón

Pérez-García

Abalo

2015

Neurogastroenterology Motil

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“…The volume of the gastiic contents were assessed by a double sampling dye dilution technique (George, 1968) at 10-minute intervals for the first 100 minutes and thereafter at 20-minute intervals until the volume of the gastric contents had fallen to 20 ml. Phenol red was chosen as the dye as it is minimally absorbed or adsorbed in the stomach (Clarke and Williams, 1971).…”

Section: Gastric Emptying Testsmentioning

confidence: 99%

The effect of preserving antral innervation and of a pyloroplasty on gastric emptying after vagotomy in man

Clarke¹,

Alexander-Williams²

1973

Gut

Self Cite

123

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SUMMARY A prospective randomized trial of 40 duodenal ulcer patients is reviewed. The patients had one of four operations (selective vagotomy, proximal gastric vagotomy, selective vagotomy plus pyloroplasty, or proximal gastric vagotomy plus pyloroplasty). The gastric emptying of a hypertonic fluid meal was assessed before and three to four months after operation. Selective vagotomy without a drainage procedure results in gastric retention and should no longer be considered as a method of treatment for duodenal ulcer. Proximal gastric vagotomy without a drainage procedure does not lead to gastric retention. Initial gastric emptying is more rapid after proximal gastric vagotomy but the final emptying time is the same as before operation and this operation alters the pattern of gastric emptying less than the other operations. Pyloroplasty added to either selective or proximal gastric vagotomy results in loss of the normal regulation of gastric emptying, very rapid initial gastric emptying, and a significant increase in the incidence of 'dumping'. It appears from these studies that 'dumping' is due to rapid gastric emptying and mainly due to the drainage procedure.

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“…Subsequent analyses showed that 98 % dry weight of the preparation reacted with Biuret reagent (Dittebrandt, 1948 (Donaldson & Barreras, 1966). In the past, however, it has been suggested that small quantities of 61CrC13 became adsorbed to intestinal mucous (Clark & Williams, 1971 Dittebrandt (Dittebrandt, 1948) using a bovine albumin standard, reading at 555 nm on a spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

Protein digestion and absorption in the rat.

Curtis¹,

Kim²,

Perdomo³

et al. 1978

The Journal of Physiology

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The value of phenol red and chromic chloride as nonabsorbable gastric indicators

Cited by 8 publications

References 5 publications

X‐ray analysis of gastrointestinal motility in conscious mice. Effects of morphine and comparison with rats

X‐ray analysis of gastrointestinal motility in conscious mice. Effects of morphine and comparison with rats

The effect of preserving antral innervation and of a pyloroplasty on gastric emptying after vagotomy in man

Protein digestion and absorption in the rat.

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