The value of PCR-RFLP molecular markers for the differentiation of immature stages of two necrophagous flies (Diptera: Calliphoridae) of potential forensic importance

Thyssen, Patrícia Jacqueline; Lessinger, Ana Cláudia; Azeredo-Espin, Ana Maria Lima de; Linhares, Arício Xavier

doi:10.1590/s1519-566x2005000500009

Cited by 21 publications

(14 citation statements)

References 6 publications

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“…; Thyssen et al. ). In addition, a large number of species‐specific COI sequences (nearly 150,000 species) have now been submitted to public sequence databases such as GenBank or The Barcode of Life Data System (BOLD) for use in taxonomic and phylogenetic studies of insects (Kwong et al.…”

Section: Introductionmentioning

confidence: 97%

“…A basic approach to develop such type test has been to employ universal PCR primers to amplify the COI gene and to subject the products to restriction fragment length polymorphism (RFPL) analysis (Vidigal et al 2002;Caldeira et al 2003;Thyssen et al 2005). In addition, a large number of species-specific COI sequences (nearly 150,000 species) have now been submitted to public sequence databases such as GenBank or The Barcode of Life Data System (BOLD) for use in taxonomic and phylogenetic studies of insects (Kwong et al 2012;Ptaszy nska et al 2012;Smith et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Reliable molecular identification of nine tropical whitefly species

Ovalle

Parsa

Hernández

et al. 2014

Ecology and Evolution

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The identification of whitefly species in adult stage is problematic. Morphological differentiation of pupae is one of the better methods for determining identity of species, but it may vary depending on the host plant on which they develop which can lead to misidentifications and erroneous naming of new species. Polymerase chain reaction (PCR) fragment amplified from the mitochondrial cytochrome oxidase I (COI) gene is often used for mitochondrial haplotype identification that can be associated with specific species. Our objective was to compare morphometric traits against DNA barcode sequences to develop and implement a diagnostic molecular kit based on a RFLP-PCR method using the COI gene for the rapid identification of whiteflies. This study will allow for the rapid diagnosis of the diverse community of whiteflies attacking plants of economic interest in Colombia. It also provides access to the COI sequence that can be used to develop predator conservation techniques by establishing which predators have a trophic linkage with the focal whitefly pest species.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Reliable molecular identification of nine tropical whitefly species

Ovalle

Parsa

Hernández

et al. 2014

Ecology and Evolution

View full text Add to dashboard Cite

show abstract

“…In turn, mitochondrial genes such as COI can be employed for other molecular techniques, such as ‘Restriction Fragments Length Polymorphism’ (RFLP) (Behura, 2006). RFLP technique has been already used for the classification of different insects of difficult morphological identification (Schroeder et al , 2003; Thyssen et al , 2005; Nagoshi et al , 2011; Ming et al , 2014). This is an efficient technique, and is more cost-effective and rapid than DNA sequencing.…”

Section: Introductionmentioning

confidence: 99%

A molecular framework for the identification of planthopper vectors (Hemiptera: Delphacidae) of central Argentina

Argüello

Dumón

Mattio

et al. 2015

Bull. Entomol. Res.

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Planthoppers are important worldwide crop pests as well as vectors of numerous diseases. Different species transmit Mal de Río Cuarto virus, which causes the most economically important corn disease in central Argentina. Epidemiological studies rely on the accurate identification of the species present in the field. Presently, morphological identification of planthoppers requires taxonomic expertise and there are no taxonomic keys for females and nymphs. Nevertheless, no molecular protocols are available for accurate species identification of most frequent delphacid species from central Argentina. In this context, the aim of this study was to evaluate the utility of the cytochrome oxidase I gene (COI) as a DNA barcode and its digestion with restriction enzymes (Restriction Fragment Length Polymorphism, RFLP) for the identification of the most common species of planthoppers in central Argentina. We amplified and sequenced a 843 bp fragment of the COI gene of taxonomically identified specimens and evaluated its use as a DNA barcode. Restriction enzymes were also selected for digesting the COI fragment via RFLP. The high interspecific variability (20.79%; ± 2.32%) and low intraspecific divergence (0.12%; ± 0.17%) observed in the studied species, demonstrate the effectiveness of the COI gene for species identification of major vector delphacids affecting corn crops in Argentina. Moreover, the digestion of this COI gene fragment with Bfa I and Apo I enzymes allows a fast and cost-effective species identification method when numerous specimens need to be processed. Both molecular techniques developed here, allow the accurate identification of planthopper species at regional scale. These new tools would assist traditional identification of these insects, especially for aiding non-experts in morphological taxonomy.

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“…A Entomologia Forense consiste na aplicação do estudo destes insetos na elucidação de questões judiciais, como morte violenta, tráfico de entorpecentes, maus tratos em idosos, crianças ou incapacitados, danos em bens móveis, dentre outros 4,5 . Além disso, os insetos podem ser usados ainda para a determinação do intervalo post-mortem (IPM) ou cronotanatognose, pois esses insetos colonizam a matéria orgânica em decomposição, indicando assim o tempo de decomposição de cadáveres 6 .…”

Section: Introductionunclassified

Estudo Preliminar da Fauna Necrófaga de Interesse Forense Associada a Fatores Abióticos no Município de Natal/RN

Cerejeira¹,

Guerreiro²,

Freitas³

2014

BJFS

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Resumo. O estudo proposto refere-se a uma área em crescente desenvolvimento no país, conhecida como Entomologia Forense. Por ser uma área relativamente nova dentro das Ciências Forenses, são escassos os registros da biodiversidade necrófaga em Natal/RN. Esta pesquisa realizou um levantamento das principais famílias de interesse forense, associando-as ao fator abiótico "insolação", verificando como os fatores "sol" e "sombra" podem interferir no processo de sucessão ecológica e decomposição. Para isso foram expostas iscas de carne bovina decomposta, em área de mata do 7º Batalhão de Engenharia de Combate de Natal/RN. O experimento ocorreu durante o outono, sendo expostas 02 armadilhas sob a condição de sol e 02 sob a condição de sombra. Coletou-se um total de 429 exemplares nas quatro armadilhas, sendo 343 dípteros muscoides adultos e 30 artrópodes pertencentes a outras famílias (formigas e abelhas) e 56 não foram identificadas ou estavam deterioradas. Sendo assim, enfatiza-se a importância deste e de outros estudos mais aprofundados sobre a fauna necrófaga nessa região, visto que o presente trabalho foi apenas uma introdução no conhecimento dos fatores sol/sombra como interferentes no processo de decomposição e abundância de insetos nessa área de mata da região.Abstract. The proposed study refers to an area of increasing development in the country, known as forensic entomology. As a relatively new area within the forensic sciences, there are few records of scavenger biodiversity in Natal/RN. This research conducted a survey of the major families of forensic interest, as related to the abiotic factor "insolation", checking factors such as "sun" and "shade" may interfere with the ecological succession and decomposition process. For that lures beef decomposed in the forest area of the 7th Engineer Battalion Combat Natal / RN were exposed. The experiment took place during the fall, with 02 traps set out under the condition of sun and 02 under the condition of shade. He collected a total of 429 copies in four traps flies Muscoid being 343 adults and 30 families belonging to other arthropods (ants and bees) and 56 were not identified or were damaged.Thus , we emphasize the importance of this and more in-depth studies on the scavenger fauna in this region , since the present study was only an introduction to the knowledge of the factors sun / shade as interfering in the process of decomposition and insect abundance in this area forest in the region.

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The value of PCR-RFLP molecular markers for the differentiation of immature stages of two necrophagous flies (Diptera: Calliphoridae) of potential forensic importance

Cited by 21 publications

References 6 publications

Reliable molecular identification of nine tropical whitefly species

Reliable molecular identification of nine tropical whitefly species

A molecular framework for the identification of planthopper vectors (Hemiptera: Delphacidae) of central Argentina

Estudo Preliminar da Fauna Necrófaga de Interesse Forense Associada a Fatores Abióticos no Município de Natal/RN

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