The value of non-culture techniques for diagnosis ofChlamydia trachomatis infections: Making the best of a bad job

Taylor‐Robinson, David

doi:10.1007/bf01960803

Cited by 22 publications

(5 citation statements)

References 39 publications

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“…Different amplification protocols were used for each transport medium, 2SP 3. In one case, the Amplicor assay was initially negative with an OD value below the cut off (repeated three times) and became positive with an OD value >3 after dilution of the sample.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Amplicor Chlamydia trachomatis test versus culture in genital samples in various prevalence populations.

Barbeyrac

Pellet²,

Dutilh³

et al. 1994

Sexually Transmitted Infections

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Objective-To evaluate a newly developed polymerase chain reaction (PCR) assay, Amplicor C trachomatis for the detection of C trachomatis in genital samples using cell culture for compari- Results-After analysis of discrepancies, the revised sensitivity and specificity of PCR were 95.3% and 100% and the positive and negative predictive values were 100% and 99.5%, respectively. Conclusion-The present results indicate that the Amplicor assay is rapid, specific and more sensitive than the culture method. This test provides an excellent non-culture method for the detection of C trachomatis in various prevalence populations.

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Section: Introductionmentioning

confidence: 99%

Evaluation of the Amplicor Chlamydia trachomatis test versus culture in genital samples in various prevalence populations.

Barbeyrac

Pellet²,

Dutilh³

et al. 1994

Sexually Transmitted Infections

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show abstract

“…After 25 repeated cycles of high temperature template denaturation, oligonucleotide primer annealing and polymerase-mediated extension, a 100,000-fold increase in the DNA sequence under investigation may be achieved, such that it then becomes easily detectable by relatively simple laboratory procedures. Most workers who have developed`inhouse' PCR tests have found that they have high speci® city and sensitivity 21 . Such tests are more sensitive than culture and, therefore, likely to be looked upon favourably, particularly if it is possible to detect one elementary body.…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Tests for infection with Chlamydia trachomatis

Taylor‐Robinson

1996

Int J STD AIDS

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“…The reliability of the GP was determined by a number of com parison studies using most often cell culture techniques. Data observed in most of the studies report the GP as being less sensitive or equal when compared with cell culture or other diagnostic procedures [7][8][9][10][11][12][20][21][22]. It was con sidered as an alternative diagnostic method for C. tracho matis when specialized equipment necessary for culturing the microorganism is not available [10].…”

Section: Discussionmentioning

confidence: 99%

Rapid Diagnosis of <i>Chlamydia trachomatis</i> with a Nucleic Acid Probe in Male and Female Patients

Stary¹,

Köpp²,

Zahel³

et al. 1994

Dermatology

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To evaluate a commercially available DNA hybridization test, the Gen-Probe Pace 2 assay (GP) was compared with the Chlamydiazyme (CZ) for the detection of Chlamydia trachomatis in urethral and endocervical specimens. Samples were collected from 300 persons, including 199 registered and 43 nonregistered female prostitutes, examined for screening purposes, as well as 22 male and 36 female symptomatic sexually transmitted disease (STD) patients. The overall prevalence of C. trachomatis was 7.3% in all persons examined with an infection rate of 20.9% in the nonregistered prostitutes, 2.5% in the registered prostitutes and 13.8% in the STD patients. The overall concordance of both diagnostic methods was 98.7% in all samples examined, 100% in male and 98.6% in female samples. Specimens with discordant results were further analyzed by a direct immunofluorescence test (MicroTrak) and by the probe competition assay (PCA). All samples only positive in the GP assay could be confirmed by the PCA while one result which was positive in the CZ could not be confirmed by any other test. The GP assay was superior to the CZ, when compared with true-positive and -negative results. The data demonstrate that the GP assay can be recommended as an alternative diagnostic technique to the CZ for Chlamydia diagnosis.

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The value of non-culture techniques for diagnosis ofChlamydia trachomatis infections: Making the best of a bad job

Cited by 22 publications

References 39 publications

Evaluation of the Amplicor Chlamydia trachomatis test versus culture in genital samples in various prevalence populations.

Evaluation of the Amplicor Chlamydia trachomatis test versus culture in genital samples in various prevalence populations.

Tests for infection with Chlamydia trachomatis

Rapid Diagnosis of <i>Chlamydia trachomatis</i> with a Nucleic Acid Probe in Male and Female Patients

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