The value of mutants unable to carry out photorespiration

Blackwell, R. D.; Murray, A. J. S.; Lea, Peter J.; Kendall, A. C.; Hall, N. P.; Turner, J. C.; Wallsgrove, R. M.

doi:10.1007/978-94-009-2269-3_33

Cited by 9 publications

(15 citation statements)

References 67 publications

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“…By using the oxygen sensitivity as a specific and typical feature of mutants defective in photorespiration, an extensive collection of barley (Hordeum vulgare) mutants was generated. This collection has underpinned the necessity for photorespiration in a second C 3 species (Blackwell et al 1988a). Additionally, photorespiratory mutants have also been isolated from many other crop plants, such as pea (Pisum sativum; Blackwell et al 1987aBlackwell et al , 1988a, tobacco (Nicotiana sylvestris and N. tabacum; Wallsgrove et al 1986;McHale et al 1988), potato (Solanum tuberosum; Heineke et al 2001;Schjoerring et al 2006) and rice (Oryza sativa; Xu et al 2009) (for overview see Table 1).…”

Section: The Significance Of Photorespiration For Photosynthetic Orgamentioning

confidence: 99%

“…Remarkably, two studies have been published showing that not all of the photorespiratory mutants could be recovered at high CO 2 concentrations, as originally defined by the term 'photorespiratory phenotype'. For this reason, these mutants were not identified using classical screening methods (Blackwell et al 1988a;Somerville 2001). One example results from characterisation of the first genetically defined glycine decarboxylase (GDC) knockout plants.…”

Section: Heavy Photorespiratory Phenotypesmentioning

confidence: 99%

“…Bleaching of seedlings in air and their recovery under high CO 2 was used as a photorespiration-specific mutant feature. Since that time, this universal 'photorespiratory phenotype' was used to identify unknown components of the photorespiratory cycle and associated processes (Blackwell et al 1988a;Somerville 2001). Moreover, using this mutant collection, the crucial role of photorespiration for normal plant development could be demonstrated, unambiguously.…”

Section: Introductionmentioning

confidence: 99%

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The variety of photorespiratory phenotypes – employing the current status for future research directions on photorespiration

Timm

Bauwe

2012

Plant Biol J

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Mutations of genes encoding for proteins within the photorespiratory core cycle and associated processes are characterised by lethality under normal air but viability under elevated CO2 conditions. This feature has been described as 'the photorespiratory phenotype' and assumed to be distinctly equal for all of these mutants. In recent years a broad collection of photorespiratory mutants has been isolated, which has allowed a comparative analysis. Distinct phenotypic features were observed when Arabidopsis thaliana mutants defective in photorespiratory enzymes were compared, and during shifts from elevated to ambient CO2 conditions. The exact reasons for the mutant-specific photorespiratory phenotypes are mostly unknown, but they indicate even more plasticity of photorespiratory metabolism. Moreover, a growing body of evidence was obtained that mutant features could be modulated by alterations of several factors, such as CO2 :O2 ratios, photoperiod, light intensity, organic carbon supply and pathogens. Hence, systematic analyses of the responses to these factors appear to be crucial to unravel mechanisms how photorespiration adapts and interacts with the whole cellular metabolism. Here we review current knowledge regarding photorespiratory mutants and propose a new level of phenotypic sub-classification. Finally, we present further questions that should be addressed in the field of photorespiration.

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Section: The Significance Of Photorespiration For Photosynthetic Orgamentioning

confidence: 99%

Section: Heavy Photorespiratory Phenotypesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The variety of photorespiratory phenotypes – employing the current status for future research directions on photorespiration

Timm

Bauwe

2012

Plant Biol J

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“…Such mutants have been selected on the basis that they grow normally under nonphotorespiratory conditions (0 7% CO2), but show severe stress symptoms and chlorotic lesions on exposure to air. The properties of the mutants have been discussed previously (Somerville 1986;Blackwell et al 1988a;Lea et al 1992) and will only be described briefly in this section.…”

Section: The Analysis Of Mutants Lacking the Enzymes Of Ammonia Assimmentioning

confidence: 99%

The use of mutants and transgenic plants to study amino acid metabolism

Lea

Forde

1994

Plant Cell & Environment

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Mutants of higher plants with alterations in amino acid metabolism have now been available for 20 years. Following the realization that at least four distinct classes of herbicides (phosphinothricins, glyphosates, imidazolinones and sulphonylureas) act by the inhibition of amino acid biosynthesis, mutants resistant to the herbicides have also been obtained. More recently, transgenic plants containing altered levels of enzymes of amino acid biosynthesis have been constructed. In this article, we have attempted to review several areas of amino acid biosynthesis including ammonia assimilation, the aspartate pathway, branched chain amino acids, aromatic amino acids and proline.

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“…To test this hypothesis, we selected the mitochondrial enzyme glycine decarboxylase (GDC) for an overexpression experiment. This particular enzyme was a prime candidate because it produces the photorespiratory CO 2 [23] and because the leaf glycine level is known as a sensitive indicator of altered photorespiratory carbon flow [24]. Mechanistically, GDC is a four-protein system comprising three enzymes (P-protein, T-protein, and L-protein) plus H-protein, a small lipoylated protein that commutes from one enzyme to the other.…”

Section: Introductionmentioning

confidence: 99%

Glycine decarboxylase controls photosynthesis and plant growth

et al. 2012

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a b s t r a c tPhotorespiration makes oxygenic photosynthesis possible by scavenging 2-phosphoglycolate. Hence, compromising photorespiration impairs photosynthesis. We examined whether facilitating photorespiratory carbon flow in turn accelerates photosynthesis and found that overexpression of the H-protein of glycine decarboxylase indeed considerably enhanced net-photosynthesis and growth of Arabidopsis thaliana. At the molecular level, lower glycine levels confirmed elevated GDC activity in vivo, and lower levels of the CO 2 acceptor ribulose 1,5-bisphosphate indicated higher drain from CO 2 fixation. Thus, the photorespiratory enzyme glycine decarboxylase appears as an important feed-back signaller that contributes to the control of the Calvin-Benson cycle and hence carbon flow through both photosynthesis and photorespiration.

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The value of mutants unable to carry out photorespiration

Cited by 9 publications

References 67 publications

The variety of photorespiratory phenotypes – employing the current status for future research directions on photorespiration

The variety of photorespiratory phenotypes – employing the current status for future research directions on photorespiration

The use of mutants and transgenic plants to study amino acid metabolism

Glycine decarboxylase controls photosynthesis and plant growth

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