Tillage is an agrotechnical practice that strongly affects the soil environment. Its effect on soil properties depends on the system and, more specifically, on the degree of soil inversion and loosening. Strip-till is a non-inversive method that loosens only narrow soil strips. In strip-till one-pass (ST-OP) technology, tillage is combined with a simultaneous application of fertilizers and seed sowing. In a static multi-year field experiment, the soil properties after application of ST-OP for 8 years were compared to those of soil under conventional tillage with the use of a moldboard plough to a depth of 20 cm (CT), and equally deep loosened and mixed reduced tillage (RT). A field experiment of these three treatments was performed since 2012 in sandy loam soil, Luvisol. A total of 44 features were examined that described the physical, chemical, biological, and biochemical soil properties in the 0–20 cm layer, and penetration resistance (PR), bulk density (BD), and soil moisture (SM) in the 25–30 cm layer. The influence of the ST-OP technology on the yield of crops was also determined. Multivariate analysis shows that the ST-OP method, in terms of affecting the soil properties, differs considerably from RT and CT treatments. The soil after the ST-OP method contained two- to four-fold more earthworms (En), with a mass (Em) 2- to 5-fold higher, than those in the soil following RT and CT, respectively. In the ST-OP soil the content of available phosphorus (Pa) and available potassium (Ka); the total count of bacteria (Bt), cellulolytic microorganisms (Bc), and fungi (Ff); and the activity of phosphatases (AlP, AcP) were significantly higher. Compared with CT, the content of total organic carbon (Ct) and its content in the fractions of organic matter were also higher, with the exception of humins (CH). The yields of winter rapeseed and winter wheat using the ST-OP technology were marginally higher compared with those using the CT and RT technology.