The vacuolar symplast: Does it really exist in plants? (Comments on Publications of G.A. Velikanov et al. in Russian Journal of Plant Physiology)

Andreev, I. M.

doi:10.1134/s1021443708060150

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…These researchers observed that the diffusion rate of the slow component decreased compared to the control but was re‐established after a consecutive treatment with a Hg scavenging agent that unblocked the channels. Still controversial research indicates that vacuoles may be interconnected what could also regulate water permeability (Velikanov and others 2005, Andreev 2008) besides that of the tonoplast.…”

Section: Resultsmentioning

confidence: 99%

¹H‐NMR Study of the Impact of High Pressure and Thermal Processing on Cell Membrane Integrity of Onions

González¹,

Barrett²,

McCarthy³

et al. 2010

Journal of Food Science

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Proton nuclear magnetic resonance (¹H-NMR) relaxometry was used to study the effects of high pressure and thermal processing on membrane permeability and cell compartmentalization, important components of plant tissue texture. High pressure treated onions were subjected to pressure levels from 20 to 200 MPa at 5 min hold time at initial temperatures of 5 and 20 °C. Thermally treated onions were exposed for 30 min at temperatures from 40 to 90 °C. Loss of membrane integrity was clearly shown by changes in transverse relaxation time (T(2)) of water at temperatures of 60 °C and above. Destabilization effects on membranes exposed to high pressure were observed at 200 MPa as indicated by T(2) measurements and cryo-scanning electron microscopy (Cryo-SEM). T(2) relaxation successfully discriminated different degrees of membrane damage based on the T(2) shift of the vacuolar component. Analyses of the average water self-diffusion coefficient indicated less restricted diffusion after membrane rupture occurred in cases of severe thermal treatments. Milder processing treatments yielded lower average diffusion coefficients than the controls. ¹H-NMR proved to be an effective method for quantification of cell membrane damage in onions and allowed for the comparison of different food processes based on their impact on tissue integrity.

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Section: Resultsmentioning

confidence: 99%

¹H‐NMR Study of the Impact of High Pressure and Thermal Processing on Cell Membrane Integrity of Onions

González¹,

Barrett²,

McCarthy³

et al. 2010

Journal of Food Science

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The Organization of Controller Motifs Leading to Robust Plant Iron Homeostasis

et al. 2016

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Iron is an essential element needed by all organisms for growth and development. Because iron becomes toxic at higher concentrations iron is under homeostatic control. Plants face also the problem that iron in the soil is tightly bound to oxygen and difficult to access. Plants have therefore developed special mechanisms for iron uptake and regulation. During the last years key components of plant iron regulation have been identified. How these components integrate and maintain robust iron homeostasis is presently not well understood. Here we use a computational approach to identify mechanisms for robust iron homeostasis in non-graminaceous plants. In comparison with experimental results certain control arrangements can be eliminated, among them that iron homeostasis is solely based on an iron-dependent degradation of the transporter IRT1. Recent IRT1 overexpression experiments suggested that IRT1-degradation is iron-independent. This suggestion appears to be misleading. We show that iron signaling pathways under IRT1 overexpression conditions become saturated, leading to a breakdown in iron regulation and to the observed iron-independent degradation of IRT1. A model, which complies with experimental data places the regulation of cytosolic iron at the transcript level of the transcription factor FIT. Including the experimental observation that FIT induces inhibition of IRT1 turnover we found a significant improvement in the system’s response time, suggesting a functional role for the FIT-mediated inhibition of IRT1 degradation. By combining iron uptake with storage and remobilization mechanisms a model is obtained which in a concerted manner integrates iron uptake, storage and remobilization. In agreement with experiments the model does not store iron during its high-affinity uptake. As an iron biofortification approach we discuss the possibility how iron can be accumulated even during high-affinity uptake.

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Gradient NMR Method for Studies of Water Translational Diffusion in Plants

Анисимов

2021

Membranes

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The review of a retrospective nature shows the stages of development of the spin-echo NMR method with constant and pulsed gradient of the magnetic field (gradient NMR) for the study of water diffusion in plant roots. The history of the initial use of gradient NMR for plants, in which it was not possible to experimentally confirm the bound state of water in cells, is described. The work presents the main ideas on which the technology of measuring diffusion by the spin-echo NMR method is built. Special attention is paid to the manifestations and record of the restricted diffusion phenomenon, permeability of membranes, along with the finite formulae used in real experiments. As examples, it gives the non-trivial results of studies of water transfer in roots through the symplastic system, from cell to cell through intercellular contacts with plasmodesmata, through aquaporins, transfer under the influence of changes in external pressure, and the composition of the gas atmosphere.

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The vacuolar symplast: Does it really exist in plants? (Comments on Publications of G.A. Velikanov et al. in Russian Journal of Plant Physiology)

Cited by 3 publications

References 17 publications

¹H‐NMR Study of the Impact of High Pressure and Thermal Processing on Cell Membrane Integrity of Onions

¹H‐NMR Study of the Impact of High Pressure and Thermal Processing on Cell Membrane Integrity of Onions

The Organization of Controller Motifs Leading to Robust Plant Iron Homeostasis

Gradient NMR Method for Studies of Water Translational Diffusion in Plants

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The vacuolar symplast: Does it really exist in plants? (Comments on Publications of G.A. Velikanov et al. in Russian Journal of Plant Physiology)

Cited by 3 publications

References 17 publications

1H‐NMR Study of the Impact of High Pressure and Thermal Processing on Cell Membrane Integrity of Onions

1H‐NMR Study of the Impact of High Pressure and Thermal Processing on Cell Membrane Integrity of Onions

The Organization of Controller Motifs Leading to Robust Plant Iron Homeostasis

Gradient NMR Method for Studies of Water Translational Diffusion in Plants

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Product

Resources

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¹H‐NMR Study of the Impact of High Pressure and Thermal Processing on Cell Membrane Integrity of Onions

¹H‐NMR Study of the Impact of High Pressure and Thermal Processing on Cell Membrane Integrity of Onions