The V_χ genes of the L regions and the repertoire of V_χ gene sequences in the human germ line

Abstract

Only 14 of the 25 V kappa genes and pseudogenes had been found before as parts of the L regions. The cloning and linking described in the accompanying report allowed us now to assign to Lp or Ld some V kappa genes which had been found before on scattered clones. In addition the sequences of several still unknown genes are reported here, thus completing the publication of the V kappa genes of the kappa locus as far as they are potentially functional or have only one or two 1-bp defects. Of the V kappa genes of the kappa locus, 32 are potentially functional, 16 have minor defects, 3 have both potentially functional and slightly defective alleles and 25 are pseudogenes which amounts to a repertoire of 76 V kappa-related gene sequences. The V kappa genes of the L regions are, within the subgroups, particularly similar to each other, which is in part due to common evolutionary origins and in part caused by gene conversion-like events. One donor-acceptor pair could be clearly identified, since converted and not-converted alleles of the acceptor gene were found. In other cases the duplicates of the converted genes served as non-converted controls.

B cell receptors in TCL1 transgenic mice resemble those of aggressive, treatment-resistant human chronic lymphocytic leukemia

“…For humans, such analyses are robust because the number of functional germ-line V H genes is relatively few (Ϸ43 and 51 for IMGT and VBase databases, respectively), and the corresponding loci have been sequenced (26)(27)(28)(29). However, for mice, these analyses are less precise because the germ-line IgV repertoire is much larger (Ϸ300 V H genes; refs.…”

Structural peculiarities of a truncated VκIII immunoglobulin light chain in myeloma with light chain deposition disease

“…4%) was found with the germ-line gene L2. It is worth noting that SCI LC was also related to the same family of germ-line genes, family IIIa, which is often expressed in autoantibodies with rheumatoid factor activity [18,19]. Alignment of the N-terminal amino acid sequence of protein REV with known protein sequences showed that it was shorter by one residue than all previously reported -chains and begun with the normal Ile2 residue of the V III subgroup (according to Kabat's numbering [15]).…”

An Antibody Fragment from a Phage Display Library Competes for Ligand Binding to the Low Density Lipoprotein Receptor Family and Inhibits Rhinovirus Infection

“…1. The V H sequence was encoded by the DP7 segment (V H 1 family) (55); and the V L sequence by the L12a segment (V k 1 family) (56). About 5 mg of scFv7 protein was purified from 1 liter of bacterial supernatant by immobilized metal chelate affinity chromatography, and was judged to be about 90% pure, by SDS-PAGE (not shown).…”