The Utilization of the SDS-PAGE Salivary Protein Profiles for Clustering Analysis of Bali Cattle (Bos sondaicus/javanicus) and the Taurine or Zebu Cross Breeds in North Lombok District, Indonesia: A Preliminary Study

Abstract

The main aim of this study is to investigate the salivary protein profile of Bali cattle (Bos sondaicus/javanicus) and their crossbred descendents with Bos taurus or Bos indicus in the country side farm of North Lombok District, Indonesia. A total of 52 samples of saliva collected from Bali cattle, a mix of Bali cattle and taurine or zebu cattle, and a pure taurine dairy cattle, were investigated through SDS-PAGE. Intra and inter specific relationships were estimated using Jaccard's similarity and Euclidean distance index. Dendograms based on cluster analysis, and hierarchical analyses using Ward's method were developed. It was revealed that cluster analyses of salivary proteins in the range of 17-45 kDa were able to be classified and disengaged from the breed or species in the population in this study. It can thus be concluded that saliva has a good prospect as an alternative biological material for phylogenetic studies in the population. It is explicable that further verification using molecular genetics technology, should be carried out before the concept is decided to be put into daily practice.