The utilization of 2-ketogluconate byHydrogenomonas eutropha H 16

Nandadasa, H. G.; Andreesen, Marion; Schlegel, Hans-Günter

doi:10.1007/bf00696219

Cited by 11 publications

(8 citation statements)

References 18 publications

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“…The two successive oxida tions required to convert glucose to 2-ketogluconate are carried out in the periplasm by membrane-associated, pyridine nucleotide-independent, glucose and gluconate dehydrogenases (77)(78)(79). 2-Ketogluconate is transported into the cell (39,72,147,148) and then acted upon by an ATP-dependent kinase that catalyzes the formation of 2-keto-6-phosphogluconate (89,91,112). The last step, conversion of 2-keto-6-phosphogluconate to 6-phosphogluconate, re quires expenditure of NAD(P)H (50,89,112).…”

Section: Alternative Routes Of Conversion Of Glucose To 6-phosphoglucmentioning

confidence: 99%

“…2-Ketogluconate is transported into the cell (39,72,147,148) and then acted upon by an ATP-dependent kinase that catalyzes the formation of 2-keto-6-phosphogluconate (89,91,112). The last step, conversion of 2-keto-6-phosphogluconate to 6-phosphogluconate, re quires expenditure of NAD(P)H (50,89,112). To our knowledge the comple ment of enzymes comprising the segment of this pathway beyond 2ketogluconate has been demonstrated in all pseudomonads in which its pres ence has h�en tested.…”

Section: Alternative Routes Of Conversion Of Glucose To 6-phosphoglucmentioning

confidence: 99%

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Alternative Pathways of Carbohydrate Utilization in Pseudomonads

Lessie¹,

Phibbs²

1984

Annu. Rev. Microbiol.

309

142

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Section: Alternative Routes Of Conversion Of Glucose To 6-phosphoglucmentioning

confidence: 99%

Section: Alternative Routes Of Conversion Of Glucose To 6-phosphoglucmentioning

confidence: 99%

Alternative Pathways of Carbohydrate Utilization in Pseudomonads

Lessie¹,

Phibbs²

1984

Annu. Rev. Microbiol.

309

142

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“…Irrespective of the carbon source, the intracellular cAMP levels and adenylate cyclase remain constant, and external addition of cAMP does not alter the repression (20,28). In pseudomonads, organic acids are found to suppress glucose uptake and its catabolizing enzymes (6,16,17,23,32). Enzymes necessary for the utilization of amide (29), histidine (20), protocatechuate (35), and xylene (4,34) are suppressed in the presence of organic acids.…”

mentioning

confidence: 99%

Preferential Utilization of Aromatic Compounds over Glucose by Pseudomonas putida CSV86

Basu

Apte

Phale

2006

Appl Environ Microbiol

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Pseudomonas putida CSV86, a naphthalene-degrading organism, exhibited diauxic growth on aromatic compounds plus glucose, with utilization of aromatics in the first log phase and of glucose in the second log phase. Glucose supplementation did not suppress the activity of degrading enzymes, which were induced upon addition of aromatic compounds. The induction was inhibited by chloramphenicol, suggesting that de novo protein synthesis was essential. Cells showed cometabolism of aromatic compounds and organic acids; however, organic acids suppressed glucose utilization.

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“…Later, four other KGUKs were identified from: (i) the Gram-positive L . mesenteroides [19], (ii) Aerobacter aerogenes (nowadays classified as Klebsiella pneumoniae) [25], (iii) Hydrogenomonas eutropha H16 (newer and alternative designations are Ralstonia eutropha H16 or Cupriavidus necator H16) [33], and (iv) P . aeruginosa [20,28].…”

Section: Introductionmentioning

confidence: 99%

2-Ketogluconate Kinase from Cupriavidus necator H16: Purification, Characterization, and Exploration of Its Substrate Specificity

et al. 2019

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We have cloned, overexpressed, purified, and characterized a 2-ketogluconate kinase (2-dehydrogluconokinase, EC 2.7.1.13) from Cupriavidus necator (Ralstonia eutropha) H16. Exploration of its substrate specificity revealed that three ketoacids (2-keto-3-deoxy-d-gluconate, 2-keto-d-gulonate, and 2-keto-3-deoxy-d-gulonate) with structures close to the natural substrate (2-keto-d-gluconate) were successfully phosphorylated at an efficiency lower than or comparable to 2-ketogluconate, as depicted by the measured kinetic constant values. Eleven aldo and keto monosaccharides of different chain lengths and stereochemistries were also assayed but not found to be substrates. 2-ketogluconate-6-phosphate was synthesized at a preparative scale and was fully characterized for the first time.

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The utilization of 2-ketogluconate byHydrogenomonas eutropha H 16

Cited by 11 publications

References 18 publications

Alternative Pathways of Carbohydrate Utilization in Pseudomonads

Alternative Pathways of Carbohydrate Utilization in Pseudomonads

Preferential Utilization of Aromatic Compounds over Glucose by Pseudomonas putida CSV86

2-Ketogluconate Kinase from Cupriavidus necator H16: Purification, Characterization, and Exploration of Its Substrate Specificity

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