The Utility of Lymphocyte Premature Chromosome Condensation Analysis for Biological Dosimetry Following Accidental Overexposure to Ionising Radiation

Chambrette, V.; Laval, Françoise; Voisin, P.

doi:10.1093/oxfordjournals.rpd.a032613

Cited by 5 publications

(5 citation statements)

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“…Chambrette et al . [ 2 ] conducted experimental and statistical analyses to determine the required number of observed cells to obtain an acceptable precision for a biological dosimetry assay. To investigate this precision, the excess PCC fragment yield has been established by examining 10, 25, 50, 100, 150, 200, 250 hybrid cells for radiation doses up to 4 Gy.…”

Section: Resultsmentioning

confidence: 99%

“…The linear dose-effect relationship obtained in this investigation is in agreement with the results of other investigations. [ 2 11 12 ]…”

Section: Resultsmentioning

confidence: 99%

“…Using PCC assay, it may be possible to accurately detect the effect of low doses (<1 Gy) as well as high doses (>4 Gy) following acute exposure to low and high LET ionizing radiation. [ 2 ] Furthermore, it was proven that PCC has also the potential to discriminate accurately between total and partial body exposures. [ 3 ] All these circumstances led us to consider this technology as a very useful assay for triage people in mass casualty radiation accidents.…”

Section: Introductionmentioning

confidence: 99%

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Construction of Calibration Curve for Premature Chromosome Condensation Assay for Dose Assessment

Neronova

2016

Genome Integrity

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Cytogenetic dosimetry plays an important role in the triage and medical management of affected people in radiological incidents/accidents. Cytogenetic biodosimetry uses different methods to estimate the absorbed dose in the exposed individuals, and each approach has its advantages and disadvantages. Premature chromosome condensation (PCC) assay presents several advantages that hopefully fulfill the gaps identified in the other cytogenetic methods. To introduce this technique into the panel of other cytogenetic methods, a calibration curve for PCC after γ-irradiation was generated for our laboratory.

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Section: Resultsmentioning

confidence: 99%

“…The linear dose-effect relationship obtained in this investigation is in agreement with the results of other investigations. [ 2 11 12 ]…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Construction of Calibration Curve for Premature Chromosome Condensation Assay for Dose Assessment

Neronova

2016

Genome Integrity

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“…The PCC technique has already been proposed in early studies as a useful dosimetric tool for obtaining results without 48-h culture procedures (Lohman et al 1992;Darroudi et al 1998a;Chambrette et al 1999;Pantelias et al 1993;Pantelias and Maillie 1985). The primary DNA damage induced in interphase nuclei is detected as excess fragments.…”

Section: Introductionmentioning

confidence: 99%

Assessing the Applicability of FISH-based Prematurely Condensed Dicentric Chromosome Assay in Triage Biodosimetry

Suto¹,

Gotoh²,

Noda³

et al. 2015

Health Physics

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The dicentric chromosome assay (DCA) has been regarded as the gold standard of radiation biodosimetry. The assay, however, requires a 2-d peripheral blood lymphocyte culture before starting metaphase chromosome analyses to estimate biological doses. Other biological assays also have drawbacks with respect to the time needed to obtain dose estimates for rapid decision on the correct line of medical treatment. Therefore, alternative technologies that suit requirements for triage biodosimetry are needed. Radiation-induced DNA double strand breaks in G0 lymphocytes can be detected as interphase chromosome aberrations by the cell fusion-mediated premature chromosome condensation (PCC) method. The method, in combination with fluorescence in situ hybridization (FISH) techniques, has been proposed in early studies as a powerful tool for obtaining biological dose estimates without 2-d lymphocyte culture procedures. The present work assesses the applicability of FISH-based PCC techniques using pan-centromeric and telomeric peptide nucleic acid (PNA) probes in triage mode biodosimetry and demonstrates that an improved rapid procedure of the prematurely condensed dicentric chromosome (PCDC) assay has the potential for evaluating exposed radiation doses in as short as 6 h after the collection of peripheral blood specimens.

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“…In investigations on the biological effects of radiation, this technique is useful for exploring the immediate post-irradiation processes (Waldren and Johnson 1974, Pantelias and Maillie 1984, Vyas et al 1991, Darroudi and Natarajan 1993, Blakely et al 1995, Prasanna et al 1997, Darroudi et al 1998, Durante et al 1998, Chambrette et al 1999, Terzoudi and Pantelias 2006, Terzoudi et al 2008, Pathak et al 2009, Darroudi et al 2010. The PCC assay allows analysis of the kinetics of chromosome aberration formation, which is eventually recognized as dicentrics, rings and translocations in the metaphase cells Maillie 1985, Darroudi et al 1998).…”

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confidence: 99%

A Modified Protocol for Accurate Detection of Cell Fusion-Mediated Premature Chromosome Condensation in Human Peripheral Blood Lymphocytes

Suto¹,

Akiyama²,

Gotoh³

et al. 2013

CYTOLOGIA

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Summary When interphase cells are fused with mitotic cells, loosely distributing chromatins in nuclei are induced to form prematurely condensed chromosomes. In this paper we report a modified protocol to unequivocally detect prematurely condensed chromosomes in human peripheral blood lymphocytes that were fused with mitotic Chinese hamster ovary (CHO) cells. To examine cell fusion-mediated premature chromosome condensation (PCC), we conducted morphological analysis by differential interference contrast microscopy and molecular cytogenetic analysis by fluorescence in situ hybridization using pan-centromeric and telomeric peptide nucleic acid (PNA) probes. These modified procedures may serve to improve the usefulness of the technique of PCC in cytogenetic investigations.

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The Utility of Lymphocyte Premature Chromosome Condensation Analysis for Biological Dosimetry Following Accidental Overexposure to Ionising Radiation

Cited by 5 publications

References 0 publications

Construction of Calibration Curve for Premature Chromosome Condensation Assay for Dose Assessment

Construction of Calibration Curve for Premature Chromosome Condensation Assay for Dose Assessment

Assessing the Applicability of FISH-based Prematurely Condensed Dicentric Chromosome Assay in Triage Biodosimetry

A Modified Protocol for Accurate Detection of Cell Fusion-Mediated Premature Chromosome Condensation in Human Peripheral Blood Lymphocytes

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