The Utility of Is6110 Sequence Based Polymerase Chain Reaction in Comparison to Conventional Methods in the Diagnosis of Extra-Pulmonary Tuberculosis

Sekar, B; Selvaraj, Logeswari; Alexis, Alain; Ravi, Sudharshan; Arunagiri, Kavita; Rathinavel, L

doi:10.1016/s0255-0857(21)01813-2

Cited by 7 publications

(3 citation statements)

References 8 publications

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“…In our study, the real-time PCR positivity rate (38.7%) was lower than that reported in previous studies, which indicated a positivity rate that varies from 60 to 70%, but only in acid-fast bacilli (AFB) positive samples [10,12]. The low PCR-positivity of our results could be attributed to the small size of the sample [14]; absence or fewer copies of sequence IS6110 in some strains of Mycobacterium tuberculosis [10]; or DNA structural changes of paraffinembedded tissues due to prolonged formalin fixation [12]. These findings raise the possibility that some local mycobacteria might not harbor the sequence IS6110.…”

Section: Discussioncontrasting

confidence: 88%

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Immunohistochemistry and real-time Polymerase Chain Reaction: importance in the diagnosis of intestinal tuberculosis in a Peruvian population

Arevalo,

Rayme,

Ramírez

et al. 2024

BMC Gastroenterol

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Introduction The diagnosis of intestinal tuberculosis is challenging even nowadays. This study aims to report the positivity rates of new diagnostic methods such as immunohistochemistry and Real-Time Polymerase Chain Reaction in patients with intestinal tuberculosis, as well as describe the pathological and endoscopic features of intestinal tuberculosis in our population. Methods This was a retrospective observational study conducted in patients diagnosed with intestinal tuberculosis, between 2010 to 2023 from the Hospital Nacional Daniel Alcides Carrion and a Private Pathology Center, both located in Peru. Clinical data was obtained, histologic features were independently re-evaluated by three pathologists; and immunohistochemistry and real-time Polymerase Chain Reaction evaluation were performed. The 33 patients with intestinal tuberculosis who fulfilled the inclusion criteria were recruited. Results Immunohistochemistry was positive in 90.9% of cases, while real-time Polymerase Chain Reaction was positive in 38.7%. The ileocecal region was the most affected area (33.3%), and the most frequent endoscopic appearance was an ulcer (63.6%). Most of the granulomas were composed solely of epithelioid histiocytes (75.8%). Crypt architectural disarray was the second most frequent histologic finding (78.8%) after granulomas, but most of them were mild. Conclusion Since immunohistochemistry does not require an intact cell wall, it demonstrates higher sensitivity compared to Ziehl–Neelsen staining. Therefore, it could be helpful for the diagnosis of paucibacillary tuberculosis.

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Section: Discussioncontrasting

confidence: 88%

“…Real-time PCR could be used in tuberculosis diagnosis, and has a higher sensitivity and specificity than culture or stains [10]. IS6110 is a DNA target sequence present only in mycobacteria, that is commonly used for Mycobacterium tuberculosiscomplex detection by real-time PCR [11].…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemistry and real-time Polymerase Chain Reaction: importance in the diagnosis of intestinal tuberculosis in a Peruvian population

Arevalo,

Rayme,

Ramírez

et al. 2024

BMC Gastroenterol

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“…A fragment of the IS6110 insertion sequence of M. tuberculosis was amplified using polymerase chain reaction (PCR) for the cases that could not be diagnosed by ZN stain. DNA was obtained from the FF-PE tissues using the phenol-chloroform technique [18,19]. The genomic DNA concentration was calculated at 100 ng/μL for each reaction.…”

Section: Determination Of M Tuberculosis By Ziehl-neelsen Staining An...mentioning

confidence: 99%

The role of TNF receptors as mediators of chronic vasculitis in possible milder forms of the CNS tuberculosis

Rivas-Castro,

Salinas-Lara,

Sánchez Garibay

et al. 2023

J Infect Dev Ctries

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Introduction: Central nervous system (CNS) tuberculosis (TB) is the most severe form of TB due to its high mortality and functional sequelae. There are several differential diagnoses for TB; and, it can also cause secondary conditions, such as vasculitis. Methodology: 155 biopsies, corresponding to 155 different patients out of 5,386 registered biopsies from 2008-2013, met the criteria of unknown etiology vasculitis and evidence of cerebral vascular disease. These were analyzed to assess the presence of central nervous system TB. The selected cases were assessed with Suzaan Marais (SM) criteria for clinical tuberculosis. After that, Ziehl-Neelsen (ZN) staining and polymerase chain reaction (PCR) were performed to amplify a fragment of the insertion sequence IS6110 of M. tuberculosis. 21 patients met the criteria for definitive tuberculosis by ZN staining and PCR, and 2 met the criteria for possible tuberculosis. Tumor necrosis factor (TNF)-α, TNF-R1, and TNF-R2 were determined by immunohistochemistry in histological sections from formalin-fixed paraffin-embedded (FF-PE) tissues in the 23 selected patients. Results: Granulomatous TB was present in almost half of the cases. TNF-R1 and TNF-R2 were expressed mainly in blood vessels, histiocytes, and macrophages. TNF-R2 expression was higher than the other markers, which suggests an anti-inflammatory response against M. tuberculosis Conclusions: The histopathological presentation of TB is not always limited to granulomas, abscesses, or meningitis; there are also clinical presentations characterized only with chronic inflammation of nervous and vascular tissue.

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Diagnosis of tuberculosis infection based on synthetic peptides from Mycobacterium tuberculosis antigen 85 complex

Kashyap

Shekhawat

Nayak

et al. 2013

Clinical Neurology and Neurosurgery

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The Utility of Is6110 Sequence Based Polymerase Chain Reaction in Comparison to Conventional Methods in the Diagnosis of Extra-Pulmonary Tuberculosis

Cited by 7 publications

References 8 publications

Immunohistochemistry and real-time Polymerase Chain Reaction: importance in the diagnosis of intestinal tuberculosis in a Peruvian population

Immunohistochemistry and real-time Polymerase Chain Reaction: importance in the diagnosis of intestinal tuberculosis in a Peruvian population

The role of TNF receptors as mediators of chronic vasculitis in possible milder forms of the CNS tuberculosis

Diagnosis of tuberculosis infection based on synthetic peptides from Mycobacterium tuberculosis antigen 85 complex

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