The utility of IS6110 sequence based polymerase chain reaction in comparison to conventional methods in the diagnosis of extra-pulmonary tuberculosis

Sekar, B; Selvaraj, Logeswari; Alexis, Alain; Ravi, Sudharshan; Arunagiri, Kavita; Rathinavel, L

doi:10.4103/0255-0857.43575

Cited by 30 publications

(41 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results were in accordance with the results of several workers B Sekar et al 12 , Mandir verma et al 13 , Parmeet Kumar et al 14 who have reported similar sensitivity and specificity of PCR in detecting extra pulmonary tuberculosis.…”

Section: Extrapulmonary Tbsupporting

confidence: 93%

Evaluation of Serological and Molecular Methods Over Conventional Methods in Diagnosis of Pulmonary and Extra Pulmonary Tuberculosis

Verma¹,

E²,

Mittal³

et al. 2012

jemds

View full text Add to dashboard Cite

, Ph: 0091 9305943057. BACKGROUND:Despite advances in field of microbiology, diagnosis of Tuberculosis remains a challenge. Diagnosis of Extrapulmonary tuberculosis is more problematic due to low bacillary load in the specimen and difficulty in obtaining the specimen from the site of lesion in many cases. PCR and its modifications are a boon in diagnosis of tuberculosis in such cases. But serological methods like ELISA are still the first choice of small laboratories in India. AIM: Comparative evaluation of serological and molecular methods over conventional methods in diagnosis of extrapulmonary and pulmonary tuberculosis. METHODS: 170 different clinical specimens suspected of tuberculosis, (100 pulmonary and 70 Extrapulmonary) were examined by PCR using MPB 64 primer, culture and microscopy. All specimens were processed using USP methodology for inhibitors free PCR. TB Ig G, Ig M and Ig A was determined using PATHOZYME MYCO Kit. Response to ATT on clinical follow up was considered as gold standard. RESULTS: Total pulmonary specimens found positive by any of the four tests was 87 (out of 100) while that for extrapulmonary samples was 63(out of 70) . For Pulmonary Specimens the diagnostic accuracy of microscopy was 88.3%,for culture 88.3%,for ELISA 67.4% and for PCR 94.1%.For extrapulmonary samples the diagnostic accuracy of microscopy was 30.1%,for culture 49.2%,for ELISA 44.4% and for PCR 87.3%. CONCLUSION: As diagnosis by microscopy and culture are sensitive and specific, for pulmonary specimens, PCR should be kept reserved for clinically probable cases found negative by conventional tests. For Extrapulmonary specimens PCR can be used as an effective screening tool as conventional methods are mostly negative. ELISA was found to have no role in diagnosis of pulmonary TB. For Extrapulmonary TB ELISA can be used as an adjunct tool but results should be interpreted with utmost caution after full evaluation of the patient both clinically and radiologically.

show abstract

Section: Extrapulmonary Tbsupporting

confidence: 93%

Evaluation of Serological and Molecular Methods Over Conventional Methods in Diagnosis of Pulmonary and Extra Pulmonary Tuberculosis

Verma¹,

E²,

Mittal³

et al. 2012

jemds

View full text Add to dashboard Cite

show abstract

“…Whenever it was properly executed, PCR has proved to be a very sensitive test in EPTB [9][10][11][12]. In the present study, PCR could detect M. tuberculosis in 11% of the tested cases.…”

Section: Discussionmentioning

confidence: 50%

PCR as a Diagnostic Tool for Extra-Pulmonary Tuberculosis

Ajantha

Shetty²,

Kulkarni³

et al. 2013

JCDR

View full text Add to dashboard Cite

Introduction: Extra-Pulmonary Tuberculosis (EPTB) accounts for approximately 40% of the tuberculosis cases. Though it is not communicable, it is a significant cause of morbidity. This study was conducted to know the efficacy of the Polymerase Chain Reaction (PCR) as an additional tool, along with the conventional methods, in the diagnosis of EPTB. Materials and Methods:Clinical samples were collected from suspected cases of EPTB. The Ziehl-Neelsen staining (ZNS), culture on the Lowenstein-Jensen medium (LJM) and PCR testing with the use of a commercial kit were performed on the homogenized samples.Results: A total of 182 samples which were received for the molecular diagnosis of EPTB were also tested by ZNS and culture on LJM for the presence of Mycobacterium tuberculosis. Of these, 22 were positive by at least one of the tests which were used. PCR detected the maximum number of cases of EPTB, followed by culture. The results of PCR and the conventional tests were analyzed by using McNemar's test for the correlated proportions-the exact method of 'IBM SPSS Statistics 20'. The analysis showed a statistical significance.Conclusions: Whenever they are feasible, using all the available tests in combination increases the laboratory detection rates of M. tuberculosis from clinical samples. PCR must be included in the diagnostic panel of EPTB.

show abstract

“…Sekar et al(2008) 21 reported that sensitivity of PCR was 90% in their study. Different studies showed sensitivity ranged between 20% and 94% for PCR assay of extra-pulmonary specimens 22,23 .…”

Section: Discussionmentioning

confidence: 90%