The utility of embryo banking in order to increase the number of embryos available for preimplantation genetic screening in advanced maternal age patients

Orris, J.J.; Taylor, T.H.; Gilchrist, J.; Hallowell, S.V.; Glassner, M.J.; Wininger, J. David

doi:10.1007/s10815-010-9474-8

Cited by 19 publications

(15 citation statements)

References 20 publications

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“…In these studies, trypsin was used on hESC whereas our study dealt with trophectoderm cells and trypsin may not separate trophectoderm cells as easily as hESC cells. We utilized Ca 2+ /Mg 2+ -free medium because it was readily available and has been used in conjunction with CCS tests and embryo biopsy for years, and its influence on CCS results would be minimal [Orris et al, 2010]. Another problem is the difficulty in the visualization of the cells after isolation.…”

Section: Discussionmentioning

confidence: 99%

Technique to ‘Map' Chromosomal Mosaicism at the Blastocyst Stage

Taylor

Griffin

Katz

et al. 2016

Cytogenet Genome Res

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The purpose of this study was to identify a technique that allows for comprehensive chromosome screening (CCS) of individual cells within human blastocysts along with the approximation of their location in the trophectoderm relative to the inner cell mass (ICM). This proof-of-concept study will allow for a greater understanding of chromosomal mosaicism at the blastocyst stage and the mechanisms by which mosaicism arises. One blastocyst was held by a holding pipette and the ICM was removed. While still being held, the blastocyst was further biopsied into quadrants. To separate the individual cells from the biopsied sections, the sections were placed in calcium/magnesium-free medium with serum for 20 min. A holding pipette was used to aspirate the sections until individual cells were isolated. Individual cells from each section were placed into PCR tubes and prepped for aCGH. A total of 18 cells were used for analysis, of which 15 (83.3%) amplified and provided a result and 3 (16.7%) did not. Fifteen cells were isolated from the trophectoderm; 13 (86.7%) provided an aCGH result, while 2 (13.3%) did not amplify. Twelve cells were euploid (46,XY), while 1 was complex abnormal (44,XY), presenting with monosomy 7, 10, 11, 13, and 19, and trisomy 14, 15, and 21. A total of 3 cells were isolated from the ICM; 2 were euploid (46,XY) and 1 did not amplify. Here, we expand on a previously published technique which disassociates biopsied sections of the blastocyst into individual cells. Since the blastocyst sections were biopsied in regard to the position of the ICM, it was possible to reconstruct a virtual image of the blastocyst while presenting each cell's individual CCS results.

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Section: Discussionmentioning

confidence: 99%

Technique to ‘Map' Chromosomal Mosaicism at the Blastocyst Stage

Taylor

Griffin

Katz

et al. 2016

Cytogenet Genome Res

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“…In some USA centres it is routine for patients to go through four egg collections before PGS is applied. From this cohort -there is an increased chance that a 'normal' embryo will be found, leading to increased pregnancy rates per embryo transfer procedure but very low pregnancy rates per egg collection (Orris et al, 2010).…”

Section: Pgs Version 2 Comprehensive Chromosome Screeningmentioning

confidence: 99%

Preimplantation Genetic Screening

Hameed

Ali²

2015

Postgraduate Obstetrics & Gynecology

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Preimplantation genetic diagnosis was first successfully performed in 1989 as an alternative to prenatal diagnosis for couples at risk of transmitting a genetic or chromosomal abnormality, such as cystic fibrosis, to their child. From embryos generated in vitro, biopsied cells are genetically tested. From the mid-1990s this technology was employed as an embryo selection tool for patients undergoing IVF, screening as many chromosomes as possible, in the belief that selecting chromosomally normal embryos would lead to higher implantation and decreased miscarriage rates. This procedure, preimplantation genetic screening (PGS), was initially performed using FISH, but 11 randomized controlled trials of screening using FISH showed no improvement in IVF delivery rates. Progress in genetic testing has led to the introduction of array comparative genomic hybridization, quantitative PCR, and next generation sequencing for PGS, and three small randomized controlled trials of PGS using these new techniques indicate a modest benefit. Other trials are still in progress but, regardless of their results, PGS is now being offered globally. In the near future, it is likely that sequencing will be used to screen the full genetic code of the embryo.

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“…In this case, the patient gradually builds a cohort of cryopreserved embryos in storage. At the appropriate time, a cycle—including these cryopreserved embryos—is planned leading to an embryo transfer employing the best embryos [36,37]. The most important parameter for POR patients of advanced maternal age is time [38].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Second Follicular Wave Phenomenon in Natural Cycle Assisted Reproduction: A Key Option for Poor Responders through Luteal Phase Oocyte Retrieval

Sfakianoudis¹,

Simopoulou

Maziotis

et al. 2019

Medicina

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Background: Emergence of Luteal Phase Oocyte Retrieval (LuPOR) may revolutionize the practice regarding the time-sensitive nature of poor responders ascertaining a higher number of oocytes, in a shorter amount of time. This may be especially important in view of employing the approach of natural cycles for Poor Responders. We suggest the acronym LuPOR describing the clinical practice of luteal phase oocyte retrieval. The aim of the study is to offer insight regarding the identity of LuPOR, and highlight how this practice may improve management of the special subgroup of poor responders. Materials and Methods: The present retrospective observational clinical study includes the collection and statistical analysis of data from 136 poor responders who underwent follicular oocyte retrieval (FoPOR) and subsequent LuPOR in natural cycles, during their In Vitro Fertilization (IVF) treatment, from the time period of 2015 to 2018. All 136 participants were diagnosed with poor ovarian reserve (POR) according to Bologna criteria. The 272 cycles were categorized as follows: 136 natural cycles with only FoPORs (Control Group) and 136 natural cycles including both FoPORs and LuPORs. Results: Our primary results indicate no statistically significant differences with regards to the mean number of oocytes, the maturation status, and fertilization rate between FoPOR and LuPOR in natural cycles. Secondarily, we demonstrate a statistically significant higher yield of oocytes (2.50 ± 0.78 vs. 1.25 ± 0.53), better oocyte maturity status (1.93 ± 0.69 vs. 0.95 ± 0.59) and higher fertilization rate (1.31 ± 0.87 vs. 0.61 ± 0.60) in natural cycles including both FoPOR and LuPOR, when compared to cycles including only FoPOR. Conclusion: Our study may contribute towards the establishment of an efficient poor responders’ management through the natural cycle approach, paving a novel clinical practice and ascertaining the opportunity to employ oocytes and embryos originating from a luteal phase follicular wave.

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The utility of embryo banking in order to increase the number of embryos available for preimplantation genetic screening in advanced maternal age patients

Abstract: Purpose To determine if embryo banking with PGS is more optimal than proceeding with PGS regardless of embryo number.

Cited by 19 publications

References 20 publications

Technique to ‘Map' Chromosomal Mosaicism at the Blastocyst Stage

Technique to ‘Map' Chromosomal Mosaicism at the Blastocyst Stage

Preimplantation Genetic Screening

Evaluation of the Second Follicular Wave Phenomenon in Natural Cycle Assisted Reproduction: A Key Option for Poor Responders through Luteal Phase Oocyte Retrieval

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