The uteroplacental contact zone cytokine influence on NK cell cytotoxicity to trophoblasts

Bazhenov, D O; Михайлова, В. А.; Nikolaenkov, Igor; Маркова, К. Л.; Salloum, Zeina; Kogan, Igor Yu.; Gzgzyan, Aleksandr M.; Selkov, S. A.; Sokolov, D. N.

doi:10.1080/09513590.2020.1816715

Cited by 4 publications

(3 citation statements)

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“…This lack of effect could be associated with both the concentration of IFNγ used and the time of cell stimulation. Previously, we found that, in the case of the short-term cultivation of NK-92 cells and JEG-3 cells, IFNγ at a similar concentration (1000 IU/mL) caused an increase in the cytotoxic activity of NK cells compared to that of non-activated cells [36]. It has been shown that, in the case of the long-term cultivation (72 h) of JEG-3 cells in the presence of IFNγ, the expression of HLA-G by trophoblast cells is increased [37].…”

Section: Discussionmentioning

confidence: 88%

“…In the current study, we found that after 24 h of preculturing, IL-10 strongly improved the cytotoxic activity of NK-92 cells towards JEG-3 cells. It was previously established that, when IL-10 was added to a model system to assess contact cytolysis by NK cells towards trophoblast cells, it caused an increase in the cytotoxicity of NK-92 cells [36]. After preculturing with IL-10, pNK cells have been shown to possess increased lytic activity against K562 cells [40,41] and an increase in granzyme B content [41].…”

Section: Discussionmentioning

confidence: 99%

“…The cells were then centrifuged (200× g, 22 • C, 10 min) and added to the target cells at a ratio of 5:1 (effector:target), and the cytotoxic function of NK cells was assessed according to the intensity of target cell death. JEG-3 trophoblast cells were used as targets, as described previously [36].…”

Section: Evaluation Of the Cytotoxicity Of The Nk-92 Cell Line After ...mentioning

confidence: 99%

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Pro- and Anti-Inflammatory Cytokines in the Context of NK Cell–Trophoblast Interactions

Михайлова

Grebenkina

Khokhlova

et al. 2022

IJMS

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During pregnancy, uterine NK cells interact with trophoblast cells. In addition to contact interactions, uterine NK cells are influenced by cytokines, which are secreted by the cells of the decidua microenvironment. Cytokines can affect the phenotypic characteristics of NK cells and change their functional activity. An imbalance of pro- and anti-inflammatory signals can lead to the development of reproductive pathology. The aim of this study was to assess the effects of cytokines on NK cells in the presence of trophoblast cells in an in vitro model. We used TNFα, IFNγ, TGFβ and IL-10; the NK-92 cell line; and peripheral blood NK cells (pNKs) from healthy, non-pregnant women. For trophoblast cells, the JEG-3 cell line was used. In the monoculture of NK-92 cells, TNFα caused a decrease in CD56 expression. In the coculture of NK cells with JEG-3 cells, TNFα increased the expression of NKG2C and NKG2A by NK-92 cells. Under the influence of TGFβ, the expression of CD56 increased and the expression of NKp30 decreased in the monoculture. After the preliminary cultivation of NK-92 cells in the presence of TGFβ, their cytotoxicity increased. In the case of adding TGFβ to the PBMC culture, as well as coculturing PBMCs and JEG-3 cells, the expression of CD56 and NKp44 by pNK cells was reduced. The differences in the effects of TGFβ in the model using NK-92 cells and pNK cells may be associated with the possible influence of monocytes or other lymphoid cells from the mononuclear fraction.

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Section: Discussionmentioning

confidence: 88%