The uteroglobin gene region: hormonal regulation, repetitive elements and complete nucleotide sequence of the gene

Suske, Guntram; Wenz, Michael; Cato, Andrew C. B.; Beato, Miguel

doi:10.1093/nar/11.8.2257

Cited by 67 publications

(42 citation statements)

References 33 publications

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“…Region I encompasses the noncanonical TATA-box region (TACA), which is recognized by the tissuespecific factor TCF and the ubiquitous factor TPF (27), which was identified in this study as YY1. (B) Comparison of the rabbit uteroglobin (UG) TACA box (56), the cognate human (63), mouse (54), and rat (18) TATA sequences, the TATA box of the Ad2 major late promoter (6), and the TACA box of the Ad2 EIIaL promoter (21) with the TATA consensus sequence, using the weight matrix description of Bucher (6). A negative weight matrix score indicates the extent of deviation from the consensus sequence (score ϭ 0).…”

Section: Methodsmentioning

confidence: 99%

“…In the rabbit lung, the uteroglobin promoter is constitutively active at a high level (14,31), whereas it is almost silent in the endometrium but becomes dramatically activated by ovarian hormones in the preimplantation phase of pregnancy (36). In both tissues, the same defined initiation start site is used (56). DNase I footprinting (35) and promoter deletion and linker-scanning analysis (55) revealed the presence of seven functionally important elements in the promoter region up to Ϫ270 bp (Fig.…”

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Binding of YY1 to a Site Overlapping a Weak TATA Box Is Essential for Transcription from the Uteroglobin Promoter in Endometrial Cells

Klug

Beato

1996

Molecular and Cellular Biology

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The gene for rabbit uteroglobin codes for a small calcium-, steroid-, and biphenyl metabolite-binding homodimeric protein which is expressed in a variety of epithelial cell types such as Clara cells (lung) and the glandular and luminal cells of the endometrium. One important region mediating its efficient transcription in a human endometrium-derived cell line, Ishikawa, is centered around a noncanonical TATA box. Two factors, TATA core factor (TCF), expressed in cell lines derived from uteroglobin-expressing tissues, and the ubiquitously expressed TATA palindrome factor, bind to the DNA major groove at two adjacent sites within this region. Here, we report the identification of the TATA palindrome factor as the transcription/initiation factor YY1 by microsequencing of the biochemically purified factor from HeLa cells. The binding site for YY1 within the uteroglobin gene is unique in its sequence and its location overlapping a weak TATA box (TACA). Binding of YY1 was required for efficient transcription in TCF-positive Ishikawa cells, which responded only weakly to a change of TACA to TATA, although in vitro binding affinity for the TATA-box-binding protein increased by 1 order of magnitude. In contrast, in CV-1 cells, lacking TCF, binding of YY1 was not required for transcription in the context of a wild-type TACA box, whereas a change from TACA to TATA led to significantly increased reporter gene expression. DNA binding data exclude a role of YY1 in stabilizing the interaction of the TATA-box-binding protein with the uteroglobin promoter. We conclude that cell lines derived from uteroglobin-expressing tissues overcome the weak TATA box with the help of auxiliary factors, one of them being YY1.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

Binding of YY1 to a Site Overlapping a Weak TATA Box Is Essential for Transcription from the Uteroglobin Promoter in Endometrial Cells

Klug

Beato

1996

Molecular and Cellular Biology

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“…The UG gene, in all mammals including humans, consists of three exons and two introns. Its 5 0 flanking region contains several steroid hormone-response elements, which regulate the tissue-specific expression of the UG gene [13][14][15]. Moreover, a polypeptide hormone, prolactin, appears to enhance further steroid-induced UG gene-expression www.academicpress.com BBRC q Abbreviations: rhUG, recombinant human uteroglobin; sPLA 2 , soluble phospholipase A 2 ; DTNB, 5,5 0 -dithiobis-nitrobenzoate; IFNc, interferon-c; H-tag, histidine-tag; AA, arachidonic acid; NMR, nuclear magnetic resonance.…”

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Lys 43 and Asp 46 in α-helix 3 of uteroglobin are essential for its phospholipase A2 inhibitory activity

Chowdhury¹,

Mantile-Selvaggi²,

Miele³

et al. 2002

Biochemical and Biophysical Research Communications

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Uteroglobin (UG) is an anti-inflammatory, secreted protein with soluble phospholipase A 2 (sPLA 2 )-inhibitory activity. However, the mechanism by which UG inhibits sPLA 2 activity is unknown. UG is a homodimer in which each of the 70-amino acid subunits forms four a-helices. We previously reported that sPLA 2 -inhibitory activity of UG may reside in a segment of a-helix 3 that is exposed to the solvent. In addition, it has been suggested that UG may inhibit sPLA 2 activity by binding and sequestering Ca þþ , essential for sPLA 2 activation. By site-specific mutation, we demonstrate here that Lys 43 Glu, Asp 46 Lys or a combination of the two mutations in the full-length, recombinant human UG (rhUG) abrogates its sPLA 2 -inhibitory activity. We demonstrate further that recombinant UG does not bind Ca þþ although when it is expressed with histidine-tag (H-tag) it is capable of binding Ca þþ . Taken together our results show that: (i) Lys 43 and Asp 46 in rhUG are critical residues for the sPLA 2 -inhibitory activity of UG and (ii) Ca þþ -sequestration by rhUG is not likely to be one of the mechanisms responsible for its sPLA 2 -inhibitory activity. Ó 2002 Elsevier Science (USA). All rights reserved.

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“…This increase might, like the other changes occurring in foetal-lung differentiation, be induced by hormonal factors. We have therefore studied the hormonal control of the developmental changes in the expression of uteroglobin, a gene whose structure is now well known (Bailly et al, 1983;Suske et al, 1983). To carry out this study we used a tissue-culture system, a technique that has been widely used in studies of lung development (Ekelund et al, 1975;Snyder et al, 1981;Torday, 1980) and which provides a means of distinguish-* To whom all correspondence and reprint requests should be addressed.…”

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Glucocorticoids induce the expression of the uteroglobin gene in rabbit foetal lung explants cultured in vitro

Haro¹,

Nieto²

1985

Biochemical Journal

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In 27-day-old rabbit foetal lung explants cultured in vitro, the synthesis of the protein uteroglobin decreased progressively during several days of culture. Addition of glucocorticoids to the medium progressively induced the synthesis of uteroglobin in a dose-dependent manner without affecting the synthesis of total proteins. The glucocorticoid-mediated induction of uteroglobin appears mainly due to increased amounts of uteroglobin mRNA and seems to be independent of simultaneous cell proliferation, suggesting a glucocorticoid-triggered differentiation of pre-existing cells. The results suggest a major role of glucocorticoids in the developmental regulation of the uteroglobin gene in the lung.

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The uteroglobin gene region: hormonal regulation, repetitive elements and complete nucleotide sequence of the gene

Cited by 67 publications

References 33 publications

Binding of YY1 to a Site Overlapping a Weak TATA Box Is Essential for Transcription from the Uteroglobin Promoter in Endometrial Cells

Binding of YY1 to a Site Overlapping a Weak TATA Box Is Essential for Transcription from the Uteroglobin Promoter in Endometrial Cells

Lys 43 and Asp 46 in α-helix 3 of uteroglobin are essential for its phospholipase A2 inhibitory activity

Glucocorticoids induce the expression of the uteroglobin gene in rabbit foetal lung explants cultured in vitro

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