The USSR programme for breeding amphibians, including rare and endangered species

Goncharov, B. F.; Shubravy, O. I.; Serbinova, Irina; Утешев, В. К.

doi:10.1111/j.1748-1090.1989.tb03248.x

Cited by 21 publications

(13 citation statements)

References 9 publications

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“…However, these techniques are now rarely used due to concerns over disease transmission and euthanasia of the donor animal (Byrne & Silla, ; Kouba, Vance, & Willis, ). Alternatively, the administration of synthetic hormones, such as luteinizing hormone‐releasing hormone analog (LHRHa) and human chorionic gonadotropin (hCG) have been demonstrated as effective approaches for inducing spermiation (Goncharov, Shubravy, Serbinova, & Uteshev, ; Kouba, Vance, et al, ; Roth & Obringer, ).…”

Section: Introductionmentioning

confidence: 99%

“…While hCG has proven to successfully induce spermiation for a broad diversity of anurans, LHRHa has grown in popularity and is preferred by zoos and aquariums for its ability to release the animals’ own endogenous hormones (Kouba, Vance, et al, ). However, despite several reviews (e.g., Goncharov et al, ; Kouba & Vance, ; Kouba et al, ), there remains a general lack of knowledge regarding the relative efficacy of LHRHa across species (but see Goncharov et al, ). Furthermore, there is little information available on the potential variation in sperm quality in response to time after hormone administration as studies tend to focus solely on identifying peak sperm concentration.…”

Section: Introductionmentioning

confidence: 99%

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Time from injection of luteinizing hormone‐releasing hormone analog affects sperm quality in the critically endangered Mississippi gopher frog (Lithobates sevosus)

2019

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The objective of this study was to investigate variation in sperm quality metrics (motility, velocity, and concentration) in the critically endangered Mississippi gopher frog (Lithobates sevosus) over three sampling time points after a luteinizing hormonereleasing hormone analog (LHRHa) induction injection. Sperm was repeatedly collected from 11 individuals over three sampling times (30, 60, and 120 min) after injection. Variation in sperm quality was investigated using a repeated-measures mixed model approach. Repeated measures analyses of variance showed a significant effect of sampling time for percent motility and velocity. Concentration was found to be marginally related to sampling time, while progressive motility was not significantly related to time after injection. Our findings are important for optimizing assisted reproduction-related fertilization success and increasing the successful propagation of endangered species of imperiled frogs in captive breeding programs. K E Y W O R D Samphibian, captive breeding, exogenous hormones, induction, sperm quality

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Time from injection of luteinizing hormone‐releasing hormone analog affects sperm quality in the critically endangered Mississippi gopher frog (Lithobates sevosus)

2019

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“…Sperm have been obtained after hormonal treatment from at least 10 species of bufonids originating from the American continent, these are: Anaxyrus americanus, A. baxteri, A. boreas, A. fowleri, A. houstonensis, Atelopus zekeri, Incilius valliceps, Rhinella arenarum, R. marina and Peltophryne lemur [13, 16, 19–25] and one from Europe and north of Africa; Bufo bufo [11, 26]. Two primary hormones that have shown success in stimulating spermiation in bufonids include Gonadotropin releasing hormone (GnRH) or its synthetic analog called Luteininzing hormone-releasing hormone (LHRH) [16, 19–22, 24–27] and human Chorionic Gonadotropin (hCG) [13, 16, 22, 25]. In direct comparison to each other, a higher concentration of sperm was found in hormonal therapy using hCG for R. marina [22] and A. americanus [16] and with GnRH in A. zeteki [25].…”

Section: Introductionmentioning

confidence: 99%

Hormonal induction of spermiation in a Eurasian bufonid (Epidalea calamita)

Arregui

Diaz-Diaz

Alonso-López

et al. 2019

Reprod Biol Endocrinol

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BackgroundAmphibian diversity is declining at an alarming rate due to habitat loss, invasive species, climate change and diseases. Captive assurance colonies have been established for some species at risk; however, many species do not breed well in captivity and the development of assisted reproductive technologies (ART) is critical to help sustain genetic diversity. To date, the majority of the work has been accomplished in species from the American continent and Australia, and there is a need to address similar breeding challenges in Eurasian and African species of amphibians.MethodsThe aim of this study was to develop a hormone protocol for stimulation of spermiation in Epidalea calamita as a model for Eurasian bufonids. Hence, the effect on sperm production and quality of three doses of chorionic gonadotropin hormone (5, 10 and 15 IU hCG/g) over time (1 to 24 h) was evaluated. In addition, cold storage (at 5 °C) of sperm for 24 and 48 h and three frequencies for hormonal treatment (weekly, biweekly and monthly) were examined.ResultsHormone concentrations of 10 or 15 IU of hCG induced spermiation in 100% of males and produced sperm of comparable quality, while 5 IU hCG stimulated spermiation in only 40% of males. Total motility peaked between 1 to 4 h post-treatment with 10 IU hCG, whereas treatment with 15 IU hCG peaked between 2 to 6 h. After 24 h of cold storage total motility dropped by 20% and forward motility dropped by 10% for both the 10 and 15 IU treatments. Weekly hormone administration resulted in higher variation between trials in all motility parameters and a lower overall Total Motility and Forward Movement. Furthermore, the effect of exogenous hormone treatment overlapped between the last two trials in the weekly frequency. Sperm concentration was higher in the first trial for all frequencies but showed no differences among other trials.ConclusionsOverall, these results show that hormone concentration, time after treatment, frequency of hormone treatment and cold storage should be borne in mind when developing a hormone stimulation protocol for Eurasian amphibian species.

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“…This method has been widely used because of the ease and reliability at which high concentrations of spermatozoa can be obtained, but research in reproductive biology is now favouring the use of non-invasive techniques that can be applied to live individuals [ 4 ]. Exogenous hormones have been used to induce spermiation (sperm release) in a number of anuran [ 5 - 11 ] and urodele [ 10 , 12 , 13 ] species since the early 20 th century. One of the hormones more commonly used to induce spermiation is luteinizing hormone-releasing hormone (LHRH).…”

Section: Introductionmentioning

confidence: 99%

Effects of luteinizing hormone-releasing hormone and arginine-vasotocin on the sperm-release response of Günther's Toadlet, Pseudophryne guentheri

Silla

2010

Reprod Biol Endocrinol

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BackgroundLuteinizing hormone-releasing hormone (LHRH) is an exogenous hormone commonly used to induce spermiation in anuran amphibians. Over the past few decades, the LHRH dose administered to individuals and the frequency of injection has been highly variable. The sperm-release responses reported have been correspondingly diverse, highlighting a need to quantify dose-response relationships on a species-specific basis. This study on the Australian anuran Pseudophryne guentheri first evaluated the spermiation response of males administered one of five LHRHa doses, and second, determined whether AVT administered in combination with the optimal LHRHa dose improved sperm-release.MethodsMale toadlets were administered a single dose of 0, 1, 2, 4 or 8 micrograms/g body weight of LHRHa. A 4 micrograms/g dose of AVT was administered alone or in combination with 2 micrograms/g LHRHa. Spermiation responses were evaluated at 3, 7 and 12 h post hormone administration (PA), and sperm number and viability were quantified using fluorescent microscopy.ResultsLHRHa administration was highly effective at inducing spermiation in P. guentheri, with 100% of hormone-treated males producing sperm during the experimental period. The number of sperm released in response to 2 micrograms/g LHRHa was greater than all other doses administered and sperm viability was highest in the 1 microgram/g treatment. The administration of AVT alone or in combination with LHRHa resulted in the release of significantly lower sperm numbers.ConclusionOverall, results from this study suggest that in P. guentheri, LHRHa is effective at inducing spermiation, but that AVT inhibits sperm-release.

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The USSR programme for breeding amphibians, including rare and endangered species

Cited by 21 publications

References 9 publications

Time from injection of luteinizing hormone‐releasing hormone analog affects sperm quality in the critically endangered Mississippi gopher frog (Lithobates sevosus)

Time from injection of luteinizing hormone‐releasing hormone analog affects sperm quality in the critically endangered Mississippi gopher frog (Lithobates sevosus)

Hormonal induction of spermiation in a Eurasian bufonid (Epidalea calamita)

Effects of luteinizing hormone-releasing hormone and arginine-vasotocin on the sperm-release response of Günther's Toadlet, Pseudophryne guentheri

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